We narrowed to 682 results for: RON
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TypeCollection..., an immortalized pre-neuronal cell line that can be differentiated into neurons Coriell Institute: Patient-specific...the location and type of MECP2 mutation can be a strong predictor of symptom severity. Broadly, early gene...and cell-specific differences and correlate with neuronal maturation. Hum Mol Genet . 11, 115–124. PMID:...
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Caltech Systemic Capsids
TypeCollection...tropism - AAV.MaCPNS1, AAV.MaCPNS2 Cell type-biased: Neurons - AAV.CAP-Mac, AAV9-X1.1 For marmosets: Broad tropism... tropism - AAV.CAP-B22 Cell type-biased: Neurons - AAV.CAP-B10 Brain vascular cells - AAV.CAP-Mac For ...: Broad tropism - AAV-PHP.eB Cell type-biased: Neurons - AAV.CAP-Mac Brain vascular cells - AAV9-X1.1,...animal Brain vascular cells - AAV9-X1.1, AAV1-X1 Neurons - AAV.CAP-B10, AAV-PHP.N Broad - AAV-PHP.AX with... -
CRISPR Pooled gRNA Libraries
TypeCollection...variable 91,948 Toronto KnockOut - Version 1 1000000069 Knockout Human Moffat 3rd 12 176,500 Toronto KnockOut... 67989 Knockout Human Yusa 3rd 5 90,709 Human Interferon-Stimulated Gene CRISPR Knockout Library 125753...Knockout Mouse Superti-Furga 3rd 6 2,384 Mouse Toronto KnockOut (mTKO) CRISPR Library 159392 159393 Knockout... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...Filaments (neuronal) Nefm EGFP Anthony Brown 32909 pEGFP-mNFM Intermediate Filaments (neuronal) Nefm EGFP... proteins from the cell to the extracellular environment. Proteins are first translated at the endoplasmic...proteins that enter the secretory pathway require chaperones to help with folding or post-translational modification... -
SARS-CoV-2 Pseudotyped Virus
TypeCollection... pseudotyped virus for SARS-CoV-2 and other coronaviruses using appropriate packaging plasmids. Use the...Nemazee lab - Pseudotyped virus protocol for coronaviruses (DOCX, 184 KB) Addgene Blog Posts Viral Vectors...COVID-19 Resources and Collections COVID-19 and Coronavirus Plasmids & Resources Ginkgo Bioworks COVID-19... -
Penn Vector Core Partnership with Addgene
TypeCollection...Optogenetics Ed Boyden AV-1-PV3446 59170-AAV1 pAAV-Syn-Chronos-GFP Optogenetics Ed Boyden AV-1-PV3447 59171-AAV1...Optogenetics Ed Boyden AV-5-PV3446 59170-AAV5 pAAV-Syn-Chronos-GFP Optogenetics Ed Boyden AV-5-PV3447 59171-AAV5...-GFP Ed Boyden AV-9-PV3446 59170-AAV9 pAAV-Syn-Chronos-GFP Ed Boyden AV-1-PV3511 98926-AAV1 pAAV.CAG.GFPsm-myc.WPRE.SV40...Karl Deisseroth AV-8-PV3706 62726-AAV8 pAAV-Syn-Chronos-tdTomato Ed Boyden AV-1-35507 35507-AAV1 pAAV-Ef1a-DIO... -
Plant Plasmids and Resources
TypeCollection...includes a high efficiency intron-optimized SpCas9-coding gene, zCas9i, and an intronized Cas9 series of vectors...Cloning Chloroplast Toolbox for design of synthetic operons for chloroplast transformation and metabolic engineering... -
CRISPR Guide
TypeCollection...general repair pathways: the efficient but error-prone non-homologous end joining (NHEJ) pathway and the...to the desired edit can increase efficiency. Synchronizing cells or modulating the cell cycle can help ...exhibit almost unidirectional insertions but are more prone to off-target insertions. And in the case of both...differentiation. The system is reversible, with a companion CRISPRon to undo repression using a TET1-dCas9 fusion. ... Gelsinger, D. R., Vo, P. L. H., Klompe, S. E., Ronda, C., Wang, H. H., & Sternberg, S. H. (2024). Bacterial.... 41 (7), 968–979. PMID: 36593413 Vo, P. L. H., Ronda, C., Klompe, S. E., Chen, E. E., Acree, C., Wang... -
Retrograde AAV viral preps
TypeCollection...transgene delivery to projection neurons. Specific classes of projection neurons can be targeted by using this...Activator Optogenetics Edward Boyden 59170 pAAV-Syn-Chronos-GFP Syn Activator Optogenetics Edward Boyden 83898... -
Zhang Lab CRISPR Page
TypeCollection...PX551; Truncated MeCP2 promoter-driven SpCas9; for neuronal expression 60958 : PX552; U6 promoter-driven; ...pAAV-pMecp2-SpCas9-spA) for expressing Cas9 in neurons. SpCas9 guide acceptor: The PX552 plasmid (#60958...-mediated expression of an sgRNA targeting the neuronal-specific gene NeuN. As a control they designed... -
Ginkgo Bioworks COVID-19 Collection
TypeCollection...or sort buttons to find SARS-CoV-2 and common coronvirus plasmids based on: Gene insert (e.g. spike, envelope... Expression Systems Collection: COVID-19 and Coronavirus Plasmids & Resources , links to collections of... -
AAV Viral Preps
TypeCollection...Purification, Cell Ablation, CRISPR Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing...retrograde Retrograde AAV To target projection neurons Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1,... -
Chemogenetics Plasmids
TypeCollection... function and examine the relationship between neuronal signaling and behavior. Depending on the signaling...the opsin to either excite or inhibit the target neuron. LMO can be activated either by adding the luciferin... -
Mammalian RNAi Tools
TypeCollection...protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design ... in a new window) . Wiznerowicz, M., Szulc, J., Trono, D. (2006). Tuning silence: conditional systems ... -
Adenovirus Plasmids
TypeCollection...production of viruses containing transgene under Neuron-specific enolase (NSE) promoter Bamburg 50959 ShuttleMCP...pShuttle-CMV-F2A-T2A-Venus Shuttle For production of viruses with multicistronic expression + Venus tag; See plasmid 62622 for... -
Retrovirus Plasmids
TypeCollection...pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG Envelope VSV-G recommended for the...expression Chen 52114 pMSCV-IRES-mCherry FP MSCV A bicistronic IRES-mCherry containing retroviral plasmid Vignali... -
Open Enzyme Collection
TypeCollection...polynucleotide kinase 165521 pOpen-lambda red operon lambda red operon 165530 pOpen-T4-BGT T4-BGT beta-glucosyltransferase... -
TALEN Plasmids and Kits
TypeCollection...of the DNA-binding domain of a TALE, along with strong nuclear localisation tags and repress transcription...transfected cells. TALEN expression is driven by the strong CAG promoter or can be achieved by in vitro mRNA... -
CRISPR Plasmids - Tagging
TypeCollection...CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed a simple...homology arms. Notably, they have used Auxin-inducible degron (AID) tagging with CRISPR/Cas to generate conditional... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection...events but can improve specificity in targeted neuronal populations. Read our blog post about how to minimize...Collection Optogenetics Plasmid Collection Monosynaptic Neuronal Tracing CRISPR Plasmids and Resources Content ...