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We narrowed to 652 results for: SIM

Showing: 61 - 80 of 652 results

  1. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...and reagents from other vendors should produce similar outcomes when using these protocols. However, please...plate washer (optional) Scale Microsoft Excel or similar software Reagents Purified antigen Purified recombinant...

  2. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...Yuan, Y., Clark, P. M., Wang, Q., Martino, R. A., Sims, J. J., Bell, P., Raymond, A., Stanford, W. L., ... capsids cross the BBB via interactions with a similar protein called LY6C1. This protein is expressed...capsids were deposited by the Gradinaru lab. For simplicity, in each section we’ll list the Gradinaru capsids...Jang et al., 2023; Brown et al., 2021.) It has a similar transduction rate to AAV-PHP.eB in the brain, and...like the rhesus macaque, are approximately more similar to humans than New World Monkeys and rodents. Image...marmosets (Chuapoco et al., 2023). AAV.CAP-B10 shows similar behavior in marmosets as in mice, although the ... thymus, and transduce the heart and spleen at similar efficacy levels to AAV9 (Stanton et al., 2023)....

  6. Video Library

    Type
    Protocol
    ...pipette and avoid inconsistent pipetting with our simple tips Bacterial Transformations We walk you through...

  11. Immunocytochemistry

    Type
    Protocol
    ...and reagents from other vendors should produce similar outcomes when using these protocols. However, please...

  14. Pouring LB Agar Plates

    Type
    Protocol
    ... a stock solution of 100,000 ug/mL (100 mg/mL). Simply measure out 100 mg of ampicillin powder, add it...

  16. Western Blot

    Type
    Protocol
    ...and reagents from other vendors should produce similar outcomes when using these protocols. However, please...

  17. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...decide to do simultaneous IF instead (Figure 1B). To label all your targets in one step, you simply use a mixture... each target. Alternatively, you can perform simultaneous detection with a mixture of primary antibodies...appropriate secondary antibodies. The general process is similar to standard immunofluorescence, but with some extra...antibodies before labeling the next target. B) Simultaneous Multiplex IF, where all the primary antibodies... Brewer et al, 2022 for a sample protocol.)  Simultaneous IF Depending on your needs, your sample, and...consideration when deciding whether you can do simultaneous or sequential labeling is what conjugated secondary...with Alexa Fluor® 488) then you can’t use them simultaneously because you won’t know which label the signal...

  18. Five Popular Model Organisms

    Type
    Blog Post
    ...recapitulate in vivo conditions similar to humans. For example, due to their simplicity, yeast have become an important...often as every three weeks so scientists can simultaneously observe several generations of mice at a time... mice are more genetically and physiologically similar to humans than many other model organisms, they...study human diseases. Diseases in mice show many similar phenotypes to human diseases and have successfully...Yeast (Saccharomyces cerevisiae) Yeast, one of the simplest eukaryotic organism, is a commonly used model ...in breads and other baked goods! Yeast is cheap, simple and easy to work with as they can survive in a ...scientists to easily manipulate them. Scientists can simply inject one-celled embryos with DNA or RNA to edit...

  19. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...standardized constructs and tools for the rational and simple shuffling of DNA regions. Golden Gate Assembly ...Assembly Tool which is freely available online. Its simple interface can guide you through both experimental...with multiple point mutations NEBuilder HiFi can simplify multi-position site-directed mutagenesis by assembling...desired mutations. This same process can be used simultaneously for the generation of one or more mutations...With NEBuilder HiFi DNA assembly, it becomes as simple as mixing fragments together with linearized vector...laborious, requiring multiple cloning steps. You can simplify this process by using NEBuilder which requires...oligo. Generating an sgRNA construct becomes as simple as combining your favorite linearized plasmid with...

  20. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...confidence that they aren’t simply doing an experiment wrong. However, even simple procedures like pouring...Reproducibility Project: Cancer Biology have produced similarly low rates of reproducibility. Failed attempts ...someone else's new and incredibly useful technique simply by following the abbreviated methods section found...modified analyses on published data if you need to. Similarly, FigShare also makes it easier to share data through... Figure 1 where many scientists are performing similar experiments. Biology is messy enough that by chance...consolidate published and unpublished experiments on similar subjects in a single, searchable space. Finally... more likely that we’ll find a fluke when we’re simply trying to get our experiments to work, but part...
Showing: 61 - 80 of 652 results