...recipient institution. An authorized signatory is someone who can execute legal documents on behalf of the...

...sequence to improve binding and reproducibility. Some scientists choose to work with full length recombinant...

...improve this technology or its application? What are some current obstacles that researchers face right now...

...serves the needs of many researchers, scientists sometimes require preps beyond our current in-stock capabilities...

...disorder known as Li-Fraumeni syndrome. Recently some impacts of p53 mutations have been characterized...

...gRNA to recruit a cytidine deaminase and induce somatic hypermutation in a 100 bp window. This technique...

...derivatives can be viewed in Krueger et al, 2004. Some cell culture sera may contain tetracycline or its...

...tagging of proteins expressed from their natural chromosomal context. These CRISPR tagging methods allow for...

... and the direction and degree of skewing of X-chromosome inactivation. J Med Genet . 44, 148–152. (Link...

...use another transfection method such as cationic liposome-based transfection. Optimize transfection conditions...

...protein interactions , protein dimerization , endosomal escape of cell-penetrating peptides , and more...

... Includes tagging with a wide variety of colors, some specifically optimized for yeast Lindquist Lab Vectors...

... amino acid. Then, the tRNA is pulled into the ribosome and if the anticodon on the tRNA can bind to the...

... Mouse IgG2a 188214 Anti-GST [N100/13R ] GST Schistosoma japonicum Mouse IgG2a 188215 Anti-TrpC7 [N64A...Mouse 190521 GST scFv [N100/13] N100/13 scFv GST Schistosoma japonicum Mouse 190522 Aldh1L1 scFv [N103/31]...

...the sample, and then through the sample. However, some of that light won’t make it through the sample because...sample or b) using dyes or stains that add color to some components of the sample but not others. The light...your ocular lens. For example, if you observe something using the 10x objective and your ocular lens is...Looking through a microscope’s eyepiece can take some practice. Try to keep both eyes open and adjust ...microscope you may wish to record your observations. Some microscopes have digital cameras attached to them...

...associated with repeated transient transfection. Some lentiviral vectors deliver mammalian antibiotic ...regularly and replace the cell media. Cell death by some cells in the culture may adversely affect the surviving...selection while the population expands. At this time, some researchers reduce the concentration of the antibiotic...integration events. In other words, if an MOI >1 was used, some cells may have 1 copy of the transgene, while others...

... of the previous section so that it will contain some bacteria. Using a fresh, sterile toothpick, or freshly...bacteria overnight (12-18 hours) at 37 °C. Pro-Tip Some plasmids or bacteria need to be grown at 30 °C instead...often true for large unstable plasmids, which sometimes recombine at 37 °C. Be sure to check this before...

...determine the size of the entire construct or excise some or all of an insert from it. Before beginning your...particularly true if you receive a plasmid from someone in another lab, or dig one out of the freezer and...Although this is never an ideal cloning strategy, sometimes it cannot be avoided. If you do have to do so,...

... 37°C for 12-18 hr in a shaking incubator. Note: Some plasmids or strains require growth at 30°C. If so...by a cloudy haze in the media (see right). Notes: Some protocols require bacteria to be in the log phase...went wrong? Try growing the culture for more time. Some bacterial cultures grow more slowly. Also, bacteria...

...specific instructions so be sure to check in with someone familiar with your lab’s instrument before use....common 1.5 mL microfuge tubes used in labs, though some larger models can hold larger conical tubes. Depending...speed ranges can vary, but are typically lower than some other centrifuge types. Floor Centrifuges sit on...