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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 318 results for: atic

Showing: 61 - 80 of 318 results

  1. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... purity of the target molecule, which can be problematic for low-abundance targets or natively isolated...sample enrichment and structure determination. A) Schematic of workflow. B) Detail of spacer peptides (3HB...optimized PASSIGE plasmids here!     Figure 3: Schematic of the PASSIGE strategy. First, twin prime editing...

  2. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...them back to wild type), differentiate them into somatic cells affected by the disease and study what’s ...in the field at the time but had never been systematically analyzed: introducing silent CRISPR/Cas9 blocking...efficiently incorporated during HDR. We decided to systematically characterize this phenomenon and found a general...

  3. March for Science

    Type
    Blog Post
    ...inclusion in science, technology, engineering and mathematics (STEM) (17). This effort is two-fold. Marchers...government: 314 Action: This nonprofit named for the mathematical constant “pi” supports scientists getting involved...Studies in History and Philosophy of Science Part: Democratic values and their role in maximizing the objectivity...

  4. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...induces fast, localized microtubule severing. A) Schematic of Opto-katanin constructs and activity. EB3N-... tubulin dimers from the microtubule body. B) Schematic of Opto-katanin’s use in cells. Images adapted...Opto-RhoGEFs to control Rho GTPase activity. A) Schematic of Opto-RhoGEF design and activity. B) OptoTIAM...

  5. Zebrafish Plasmid Collection

    Type
    Collection
    ...multicellular systems typically relies on genetic markers, somatic mutations, or labeling with fluorescent proteins...

  7. Finding Your Perfect Job After University

    Type
    Blog Post
    ...Masters, because tuition fees had just gone up dramatically and, in England, you could not receive government...detection and diagnosis of different types of pancreatic cancer and highlighting which treatment would... mutations. This is a particular challenge in pancreatic cancer where one drug does not work for all patients...

  8. Antibodies 101: Validation

    Type
    Blog Post
    ...so you use your bioinformatics skills (or your friendly neighborhood bioinformatician) to look at RNA ...have relatively low sensitivity, which can be problematic if you are trying to show that your antibody ...

  9. Ras Pathway

    Type
    Collection
    ...survival, adhesion, growth, and differentiation. Schematic of Ras signaling cascade that starts at the plasma...

  10. TALEN Plasmids and Kits

    Type
    Collection
    ...Assembly via serial ligation. Validated in zebrafish somatic cells. Keith Joung Zhang Lab TALE Toolbox PCR/Golden...

  11. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...very weakly, most likely through unstructured electrostatic interactions. It also can’t recognize PAMs, ...conformation shown in Figure 3. Though Cas9 is not a static molecule in any binding state, RNA loading stabilizes...Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco...

  12. COVID-19 Resources

    Type
    Collection
    ...optimize the method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2). ...

  15. Reflections and Looking Ahead

    Type
    Blog Post
    ...launched AAV Packaged on Request, a service that dramatically expands and simplifies your viral vector options...data for over 150,000 plasmids, empowering bioinformaticians, data scientists, and developers to unlock...

  16. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...living animals, to explore pathways that confer metastatic potential or the ability to survive in certain...years. References 1. Martz, Colin A., et al. "Systematic identification of signaling pathways with potential...

  17. Luciferase Plasmid Collection

    Type
    Collection
    ...variety of organisms, ranging from bacteria to aquatic animals to insects. Luciferase catalyzes the oxidation...

  18. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...development of dCas9 and dCas9-fusion proteins has dramatically increased the potential applications of CRISPR... LOC8-ing mitochondrial fusion with a new tri-chromatic assay Georg Ramm’s lab at Monash University ...the produced luciferase is truncated and lacks enzymatic activity. The Luc plasmid (intronless) is useful...Georgieva et al developed the inntags via a bioinformatics screen for proteins with known structure and...

  19. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...situation where you need to use plasmid in TE for any enzymatic reaction, an effective possibility is to dilute...preparation in any downstream application involving enzymatic reactions, a valid alternative can be Tris buffer...

  20. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...multiple steps you could examine, i.e. for an enzymatic reaction, you could measure consumption of a substrate...biosensors. These constructs are often used to monitor enzymatic activity or phosphorylation over time. You can...
Showing: 61 - 80 of 318 results