We narrowed to 322 results for: phy
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TypeBlog Post...dicots Self-labeling tags for single-molecule biophysics Fast and responsive voltage indicator ONE-GO ... SNAP! Self-labeling tags for single-molecule biophysics By Mike Lacy In order to study the conformational...mEPSPs has been possible with conventional electrophysiological methods, it has remained difficult with ...receptors (GPCRs) are critical components of many physiological pathways and represent the largest family of...
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Magnetic Control of Proteins: More than a Dream
TypeBlog Post...to exploration and collaboration. Andrew is a physicist with a background in optics and a track record... readily admit they don’t fully understand the physical mechanism, though they have collected a variety...of fields, like quantum chemistry and protein biophysics, as well as inventors and biomedical engineers...Gating Mechanism for Magnetogenetic Ion Channels. Biophysical Journal, 116(3), 454–468. https://doi.org/10.1016... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...even when larger vector genomes are packaged, the physical size of the genomes is still ~4.7kb. But despite...-dystrophin gene to a mouse model of muscular dystrophy. While mini-dystrophin expression levels were ...overlapping split vector had an improvement in muscle physiological performance. 2. Trans-splicing Overview: For...-dystrophin gene to a mouse model of muscular dystrophy. Three different splice site donor and acceptor... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...laboratory, then FRET experiments are a viable biophysical path to answers. What is FRET and what's it...Fluorescence resonance energy transfer (FRET) is a biophysical interaction between two fluorophores. When the... In fact, FRET can be used to derive a precise physical distance between the donor/acceptor fluorophores...and purification is a snap using thin layer chromatography (Fessenden, 2009). However, these reagents ... -
Validated gRNA Sequences
TypeCollection...CAAAAGACTGTCAATTCCCT cut S. pyogenes 26479191 Kim PHYB A. thaliana CACTAGGAGCAACACCCAA cut S. pyogenes ... -
Who Gives a Tweet? 9 Facts About Scientists on Twitter
TypeBlog Post...overrepresented on Twitter Mathematical, life, and physical scientists are underrepresented. It’s important...shown. Top: The follower network of life (green), physical (blue) and social (orange) scientists largely...disciplines: social (left), life (center), and physical (right). How do you use Twitter as a scientist... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog Post...agricultural plant. Her current research focuses on endophytes, bacteria, and fungi that reside within living...antimicrobial activities (1). She has developed a fast endophyte screening system and plans to employ the light‐inducible...References 1. Martinez-Klimova, Elena et al. “Endophytes as sources of antibiotic” Biochemical Pharmacology... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog Post...behavior, or the animals being monitored were physically hooked up to invasive, cumbersome systems during...optogenetic purposes, measure temperature, study electrophysiology, and deliver small molecules. Fun fact:...fibers as previously described, then measured electrophysiological potential afterwards. This measurement trended... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post...complexity of translational control in cellular physiology. Ribosome profiling Ribosome profiling, a biochemical...translation While ribosome profiling co-opts the physical location of the ribosome, TRICK (Translating RNA...studying translation in various cell types and physiological states. References 1. Ingolia, Nicholas T.,... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post...Additionally, the Phylogeny tool allows users to browse entries by the current phylogenetic nomenclature and... CRISPR-Cas Enzymes [Preprint]. Biochemistry, Biophysics, and Structural Biology. https://doi.org/10.32942... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post...lines appearing in American Journal of Physiology-Cell Physiology over the past decade. For more practical...might suggest underlying sex-based influences on physiologic or pathologic processes. Much progress has been... -
27 Hot Plasmids from 2016
TypeBlog Post...PMID: 27579989 DECEMBER 2016 Restoring yeast prototrophy with plasmids Though auxotrophic markers are ...auxotrophic backgrounds can lead to disparate physiological effects and complicate any metabolic study. ...lab has created a unique tool with their Yeast Prototrophy Kit, a set of plasmids designed to complement... and besides being used for restoring strain prototrophy, can also aid in designing self-establishing ... pooled library Parasites from the apicomplexan phylum cause severe human and livestock diseases such ... -
CRISPR in the Clinic
TypeBlog Post...but are not limited to, ones targeting muscular dystrophy, cardiovascular disease, inflammatory diseases...editing frequency isn’t high enough to make a physiological difference, then the treatment will not work...blog CRISPR Antimicrobials Treating Muscular Dystrophy with CRISPR CRISPR 101: Drug Discovery and CRISPR... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post...the relevant volume is that of the cell. For the physical behaviors associated with a molecule’s concentration...structures are large (microns), bright, non-physiological, and difficult to ignore [1, 2]. 2. Fusion to...of fluorescent proteins to oligomerize under physiologic conditions. Traffic, 2012. 13(5): p. 643-9. PubMed... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post...expressed can cause facioscapulohumeral muscular dystrophy (FSHD), a disease for which there is currently...immunoprecipitation (enChIP) using CRISPR." Biochemical and biophysical research communications 439.1 (2013): 132-136... Fujita, Toshitsugu, et al. "Identification of physical interactions between genomic regions by enCh IP‐Seq... -
Summer SciComm Series: Modes of Communication
TypeBlog Post...Ample distribution channels both digitally and physically. Easy to incorporate visuals if needed. Traditional...academic to informal writing can difficult. Photography Medium (high for microscopy) Visually compelling...levels and interaction. Can bring in things to be physically interacted with. Public speaking is a common ... -
AAVs in Retinal Gene Therapy
TypeBlog Post...diseases such as hemophilia, muscular dystrophy, spinal muscular atrophy, neurodegenerative diseases and metabolic...life-threatening (cystic fibrosis, hemophilia, muscular dystrophy)? AAV gene therapy started in the mid-90's with... -
Getting to Know Expansion Microscopy
TypeBlog Post...be expanded to ~4-10x linearly! This expansion physically separates molecules while maintaining their relative... Figure 1: The ExM process allows you to physically magnify a sample so you don’t have to optically...ambient temperatures, incubation times, and the physical properties of the sample itself. For example, ... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post... Streptococcus pyogenes (Sp) 3' NGG Staphylococcus aureus (Sa) NGRRT or NGRRN Neisseria ... useful than SpCas9. For example, Cas9 from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller...Zhang F (2015) In vivo genome editing using Staphylococcus aureus Cas9. Nature 520:186–191 . https://doi.org... -
Hot Plasmids: Fall 2024
TypeBlog Post... forms that limit its functionality. To make biophysical and structural studies more feasible, the lab...properties, and is amenable to a wide range of biophysical assays (Kroupova et al., 2024). They were able... Cereblon construct for crystallographic and biophysical studies of protein degraders. Nat Commun., 15...