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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 526 results for: phy

Showing: 61 - 80 of 526 results

  1. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...gene. SlugCas9 is a compact Cas9 ortholog from Staphylococcus lugdunensis that recognizes a NNGG PAM and ...terminals with one vector. AAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby from Liqun Luo’s lab expresses membrane...tethered GFP to enhance axonal labeling and synaptophysin-tagged mRuby to enrich pre-synaptic labeling...targeted tdTomato and pre-synaptic targeted synaptophysin tagged eGFP. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE...available in a ready-to-use AAV5 viral vector. Synaptophysin-tagged GCaMP6s is enriched in the pre-synaptic...

  2. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...variables - your approach, your workflow, or your physical setup - to prevent the mistake, just like you ...light-sensitive reagents” or “re-sterilize now.” Have a physical copy of the protocol when you work and make a ...time to do them carefully and thoroughly. Have a physical copy on hand as you run your experiments. Try ...your plate maps onto your plates, alongside your physical copy. If you’ve got messy handwriting, reach for...accuracy.  Bench setup Small adjustments in the physical organization of your workspace and your workflow...

  3. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...across broader physical areas of the brain and enable access to neurons that are physically blocked, such...driven by neurons. By studying how neurons are physically connected, we can begin to understand how the... have identified 180 brain regions. Both this geography and the way the brain is connected to itself across...Therefore, by simply looking at how neurons are physically connected we can begin to understand how the ...

  4. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...insensitivity of yeast strains to some antibiotics. Auxotrophy is defined as the inability of an organism to... functional gene which complements the host’s auxotrophy. When grown on media NOT containing the nutrient...and provides the element needed to overcome the auxotrophy as well as additional uses for said element. ...plasmid may be expressed at higher than normal physiological levels due to high copy numbers. This creates...the presence of the selection marker at non-physiological levels. Scientists have tried varied approaches...

  5. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...gene. SlugCas9 is a compact Cas9 ortholog from Staphylococcus lugdunensis that recognizes a NNGG PAM and ...terminals with one vector. AAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby from Liqun Luo’s lab expresses membrane...tethered GFP to enhance axonal labeling and synaptophysin-tagged mRuby to enrich pre-synaptic labeling...targeted tdTomato and pre-synaptic targeted synaptophysin tagged eGFP. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE...available in a ready-to-use AAV5 viral vector. Synaptophysin-tagged GCaMP6s is enriched in the pre-synaptic...

  6. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...developments. Model organisms often feature genetic, physiological, or developmental characteristics that make ...are great mammalian models due to their size, physiology, and genetic similarities with humans. However...However, rats have some key differences in physiology, intelligence, and human correspondence that give them...norvegicus. Image from Staffan Vilcans. Rat physiology is also easier to study and at times more closely...Biotechnology. In Escherichia coli - Recent Advances on Physiology, Pathogenesis and Biotechnological Applications...

  7. Validated gRNA Sequences

    Type
    Collection
    ...CAAAAGACTGTCAATTCCCT cut S. pyogenes 26479191 Kim PHYB A. thaliana CACTAGGAGCAACACCCAA cut S. pyogenes ...

  8. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...developments. Model organisms often feature genetic, physiological, or developmental characteristics that make ...are great mammalian models due to their size, physiology, and genetic similarities with humans. However...However, rats have some key differences in physiology, intelligence, and human correspondence that give them...norvegicus. Image from Staffan Vilcans. Rat physiology is also easier to study and at times more closely...Biotechnology. In Escherichia coli - Recent Advances on Physiology, Pathogenesis and Biotechnological Applications...

  9. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...optogenetics: 1) electrophysiology and 2) behavioral experiments. I’m not an electrophysiologist so I cannot...numerous studies, optogenetics is used in slice physiology in order to directly show that, for example, ...behavior, you don’t necessarily need to use electrophysiology to prove that your optogenetic manipulations... (though a reviewer will probably ask for electrophysiology data too but that’s what collaborators are...

  12. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...dicots Self-labeling tags for single-molecule biophysics Fast and responsive voltage indicator ONE-GO ... SNAP! Self-labeling tags for single-molecule biophysics By Mike Lacy In order to study the conformational...mEPSPs has been possible with conventional electrophysiological methods, it has remained difficult with ...receptors (GPCRs) are critical components of many physiological pathways and represent the largest family of...

  13. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...to exploration and collaboration. Andrew is a physicist with a background in optics and a track record... readily admit they don’t fully understand the physical mechanism, though they have collected a variety...of fields, like quantum chemistry and protein biophysics, as well as inventors and biomedical engineers...Gating Mechanism for Magnetogenetic Ion Channels. Biophysical Journal, 116(3), 454–468. https://doi.org/10.1016...

  14. Working with Nuclear Receptors

    Type
    Blog Post
    ...and on self-propagation by reproduction. Both physiological processes are controlled by deliberate and compulsory...receptors (NRs), their coregulators, and their physiological endocrine ligands. Nuclear receptors and their...circadian regulation, as well as in important physiological states such as adipogenesis and macrophage activation...Mangelsdorf, D. J. (2001). Nuclear receptors and lipid physiology: opening the X-files. Science 294, 1866-1870....

  15. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...transduction of cells." American Journal of Physiology-Cell Physiology 291.2 (2006): C386-C392. PubMed PMID:...Different titering methods can reveal differences in physical versus functional titer. If you have low titers... virus adsorption on target cell membranes." Biophysical chemistry 97.2 (2002): 159-172. PubMed PMID: ...

  16. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...dicots Self-labeling tags for single-molecule biophysics Fast and responsive voltage indicator ONE-GO ... SNAP! Self-labeling tags for single-molecule biophysics By Mike Lacy In order to study the conformational...mEPSPs has been possible with conventional electrophysiological methods, it has remained difficult with ...receptors (GPCRs) are critical components of many physiological pathways and represent the largest family of...

  17. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...to exploration and collaboration. Andrew is a physicist with a background in optics and a track record... readily admit they don’t fully understand the physical mechanism, though they have collected a variety...of fields, like quantum chemistry and protein biophysics, as well as inventors and biomedical engineers...Gating Mechanism for Magnetogenetic Ion Channels. Biophysical Journal, 116(3), 454–468. https://doi.org/10.1016...

  18. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...even when larger vector genomes are packaged, the physical size of the genomes is still ~4.7kb. But despite...-dystrophin gene to a mouse model of muscular dystrophy. While mini-dystrophin expression levels were ...overlapping split vector had an improvement in muscle physiological performance.  2. Trans-splicing Overview: For...-dystrophin gene to a mouse model of muscular dystrophy. Three different splice site donor and acceptor...

  19. Antibody Guide

    Type
    Collection
    ...responsible for driving allergic reactions such as anaphylactic shock Monomer with a valency of 2 IgG The most...

  20. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...even when larger vector genomes are packaged, the physical size of the genomes is still ~4.7kb. But despite...-dystrophin gene to a mouse model of muscular dystrophy. While mini-dystrophin expression levels were ...overlapping split vector had an improvement in muscle physiological performance.  2. Trans-splicing Overview: For...-dystrophin gene to a mouse model of muscular dystrophy. Three different splice site donor and acceptor...
Showing: 61 - 80 of 526 results