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We narrowed to 327 results for: phy

Showing: 61 - 80 of 327 results
  1. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post
    ...laboratory, then FRET experiments are a viable biophysical path to answers. What is FRET and what's it...Fluorescence resonance energy transfer (FRET) is a biophysical interaction between two fluorophores. When the... In fact, FRET can be used to derive a precise physical distance between the donor/acceptor fluorophores...and purification is a snap using thin layer chromatography (Fessenden, 2009). However, these reagents ...
  2. Who Gives a Tweet? 9 Facts About Scientists on Twitter

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    Blog Post
    ...overrepresented on Twitter Mathematical, life, and physical scientists are underrepresented. It’s important...shown. Top: The follower network of life (green), physical (blue) and social (orange)  scientists largely...disciplines: social (left), life (center), and physical (right). How do you use Twitter as a scientist...
  3. Multifunctional Neural Fibers: Modulation from Gut to Brain

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    ...behavior, or the animals being monitored were physically hooked up to invasive, cumbersome systems during...optogenetic purposes, measure temperature, study electrophysiology, and deliver small molecules.   Fun fact:...fibers as previously described, then measured electrophysiological potential afterwards. This measurement trended...
  4. Visualizing Translation at the Single Molecule Level

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    Blog Post
    ...complexity of translational control in cellular physiology. Ribosome profiling Ribosome profiling, a biochemical...translation While ribosome profiling co-opts the physical location of the ribosome, TRICK (Translating RNA...studying translation in various cell types and physiological states. References 1. Ingolia, Nicholas T.,...
  5. CasPEDIA: A Functional Classification of Cas Enzymes

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    Blog Post
    ...Additionally, the Phylogeny tool allows users to browse entries by the current phylogenetic nomenclature and... CRISPR-Cas Enzymes [Preprint]. Biochemistry, Biophysics, and Structural Biology. https://doi.org/10.32942...
  6. Gendered Innovations: Why Does Sex of the Cell Matter?

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    Blog Post
    ...lines appearing in American Journal of Physiology-Cell Physiology over the past decade. For more practical...might suggest underlying sex-based influences on physiologic or pathologic processes. Much progress has been...
  7. 27 Hot Plasmids from 2016

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    Blog Post
    ...PMID: 27579989 DECEMBER 2016 Restoring yeast prototrophy with plasmids Though auxotrophic markers are ...auxotrophic backgrounds can lead to disparate physiological effects and complicate any metabolic study. ...lab has created a unique tool with their Yeast Prototrophy Kit, a set of plasmids designed to complement... and besides being used for restoring strain prototrophy, can also aid in designing self-establishing ... pooled library Parasites from the apicomplexan phylum cause severe human and livestock diseases such ...
  8. CRISPR in the Clinic

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    Blog Post
    ...but are not limited to, ones targeting muscular dystrophy, cardiovascular disease, inflammatory diseases...editing frequency isn’t high enough to make a physiological difference, then the treatment will not work...blog CRISPR Antimicrobials Treating Muscular Dystrophy with CRISPR CRISPR 101: Drug Discovery and CRISPR...
  9. Getting to Know Expansion Microscopy

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    Blog Post
    ...be expanded to ~4-10x linearly! This expansion physically separates molecules while maintaining their relative...   Figure 1: The ExM process allows you to physically magnify a sample so you don’t have to optically...ambient temperatures, incubation times, and the physical properties of the sample itself. For example, ...
  10. AAVs in Retinal Gene Therapy

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    Blog Post
    ...diseases such as hemophilia, muscular dystrophy, spinal muscular atrophy, neurodegenerative diseases and metabolic...life-threatening (cystic fibrosis, hemophilia, muscular dystrophy)? AAV gene therapy started in the mid-90's with...
  11. Summer SciComm Series: Modes of Communication

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    Blog Post
    ...Ample distribution channels both digitally and physically. Easy to incorporate visuals if needed. Traditional...academic to informal writing can difficult.  Photography Medium (high for microscopy) Visually compelling...levels and interaction. Can bring in things to be physically interacted with. Public speaking is a common ...
  12. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post
    ...expressed can cause facioscapulohumeral muscular dystrophy (FSHD), a disease for which there is currently...immunoprecipitation (enChIP) using CRISPR." Biochemical and biophysical research communications 439.1 (2013): 132-136... Fujita, Toshitsugu, et al. "Identification of physical interactions between genomic regions by enCh IP‐Seq...
  13. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ... Streptococcus pyogenes (Sp) 3' NGG Staphylococcus aureus (Sa) NGRRT or NGRRN Neisseria ... useful than SpCas9. For example, Cas9 from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller...Zhang F (2015) In vivo genome editing using Staphylococcus aureus Cas9. Nature 520:186–191 . https://doi.org...
  14. Hot Plasmids: Fall 2024

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    Blog Post
    ... forms that limit its functionality. To make biophysical and structural studies more feasible, the lab...properties, and is amenable to a wide range of biophysical assays (Kroupova et al., 2024). They were able... Cereblon construct for crystallographic and biophysical studies of protein degraders. Nat Commun., 15...
  15. Plasmids 101: Broad Host Range Plasmids

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    Blog Post
    ... among organisms belonging to many different phylogenetic groups (Jain and Srivastava 2013).  Recombinant...Paracoccus denitrificans, Pasteurella multocida, Porphyromonas gingivalis, Proteus mirabilis, Providencia spp...Meyer, R., Figurski, D., & Helinski, D. R. (1977). Physical and genetic studies with restriction endonucleases...
  16. Viral Vectors 101: Optogenetic Tools

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    Blog Post
    ...light, oxygen, and voltage (LOV) domains, phytochrome B (PhyB), and cryptochrome 2 (CRY2) (Zhang & Cui,...study multiple aspects of cellular activity and physiology. However, there are a number of factors that ...
  17. Antibody Guide

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    Collection
    ...responsible for driving allergic reactions such as anaphylactic shock Monomer with a valency of 2 IgG The most...
  18. Important Considerations When Using AAVs

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    Blog Post
    ...contributed by guest blogger Katrina Armstrong, a Neurophysiology Msc Student at the University of Manitoba. ...comparisons to enhance our understanding of receptor physiology and the effects of metabotropic and ionotropic... Katrina Armstrong. Katrina Armstrong is a Neurophysiology Msc Student at the University of Manitoba. ...
Showing: 61 - 80 of 327 results