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...site", "active site loop", or "subunit interface") ID Plasmid Description Gene/Insert Mutations Additional...collaborations between institutions. With these plasmids, faculty can begin a sustainable MDH-related CURE...and metabolic pathways and protein interactions. Plasmids A diverse set of wild-type MDH genes from several...page. Use the search bar or sort buttons to find plasmids based on: Organism or species (e.g., human, watermelon...

...Adapted from Park & Sheridan et al. (2025). PRISM Plasmids ID Plasmid Description Tags Additional Resources...The plasmids can be packaged as AAVs and pooled together for co-delivery. Alternatively, plasmids can ...Collection E11 Bio PRISM Collection How to Use Plasmids Additional Resources Protein-barcode Reconstruction...applications. Park & Sheridan et al. (2025) use plasmids expressing the barcode proteins under the constitutive...developed a set of Cre-dependent, hSyn-Tet inducible plasmids (Addgene #242782–242799) suitable for targeting...

...virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...Armamentarium Project below. Plasmids ID Plasmid Description PI Viral Preps ID Viral Prep Description PI ...underlie behavior and network function. Browse plasmids or viral preps created with support from the BRAIN...

...anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions for other plasmids that...Apr 8. Markus Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies... ready-to-use recombinant antibodies made from plasmids in our collection! Many of these monoclonal antibodies...More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large proteins made by the immune...antibodies, created by cloning antibody components into plasmids and expressing these in bacteria, mammalian cells...Trimmer Lab Recombinant mAb Collection . Once the plasmids are transiently transfected into mammalian tissue...nanobodies). For example, scientists often use plasmids encoding scFv fragments that bind to the GCN4 ...

... synthases needed for use of these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag...Biolabs has deposited plasmids at Addgene to generate SNAP, CLIP, ACP or MCP tagged plasmids for visualization...Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental Information New England Biolabs offers... of proteins inside living or fixed cells. The plasmids associated with this technology are available ... enzymatic binding to attach substrate Control plasmids with a recognizable sub-cellular localization ...FAQs (PDF, 252 KB) ACP and MCP tags ACP-MCP-Tag plasmids - Substrate source (PDF, 46 KB) Please note: New...

...collection (Link opens in a new window) on Promega.com. ID Plasmid Description Industry Plasmid Technology Overview...Plasmid Collection Ordering & Availability All Plasmids Plasmid Technology Overview Additional Resources...operational complexity. This unique collection contains plasmids developed by Promega scientists and designed to...HaloTag, SmBiT, and LgBiT. Ordering & Availability Plasmids from the Promega collection are available in two... DNA in Tris buffer (500 ng/µL ± 25 ng/µL) All plasmids in the collection are available to academic, nonprofit...Collection Browse and sort the table below to find plasmids in the collection. For example, search by response...complementary peptide, Small BiT (SmBiT; 11 amino acids). This system is ideal for protein:protein interaction...

...AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement... in specific cell types. See our Chemogenetics Plasmids and Chemogenetics Guide pages for more information...physically separate) none 1, 9, PHPeB Jordane Dimidschstein 52536 rAAV-CAG::FLEX-rev:: hM4D-2a-GFP hM4D(...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

... plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter PI Additional Resources...real-world troubleshooting. Eligible Plasmids The following plasmids are eligible for this service. Submit...Overview Details Ordering Share Your Results Eligible Plasmids Additional Resources Adeno-associated virus (AAV...lets scientists choose from over 4,000 transfer plasmids to be packaged in their choice of seven serotypes...optogenetic, recombinase, and biosensor transfer plasmids in our repository are eligible for this service...

...publications associated with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes...for in vivo use. Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...trusted partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity Recommended Applications...research. Browse the BRAIN Initiative collection of plasmids, viral preps, and antibodies here. Collections...dissemination to the research community. Browse plasmids , viral preps , and antibodies created with support...distributes ready-to-use viral preps made from plasmids in our collection! AAV are distributed as high-quality...control of the E2 regulatory element Jordane Dimidschstein 135630-AAV9 pAAV-S5E2-dTom-nlsdTom AAV vector...

..., and Cre expression plasmids. Large collections or kits are described below . ID Plasmid Description ... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources...some of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...highlights plasmids that contain tools for use in zebrafish research. This includes plasmids for genome...expressed. EXPRESs Plasmids for Zebrafish Receptors and Zebrafish Monoclonal Antibody plasmids - Gavin Wright...: Find plasmids for use in zebrafish. Plasmid...

...click on the plasmid name for ordering information. ID Name Promoter Description Category PI 157970 pTR-...Engineered AAV Capsids Improve Transduction and Volumetric Spread in the CNS. Mol Ther Nucleic Acids . Sep 15...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida...where the vector is diluted by the physiologic body fluids, such as blood, vitreous, CSF, etc. Not suitable...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...also like... Plasmid Kits Blog: Plasmids 101 Modular Cloning Blog: Plasmids 101 Modular Cloning Applications...Applications and Kits Blog: Plasmids 101 Golden Gate Cloning Synthetic Biology Plasmids Modular Cloning (or MoClo...functional plasmids. It is a powerful and very efficient method for creating many plasmids from different...Parrott 48 plasmids compatible with the GreenGate Cloning System (Lohmann) to create plasmids with multi-gene...multigene plasmids for genome integration in fission yeast S. pombe . These can be used with plasmids from ...flanked by fusion sites into individual Level 0 plasmids, or choose from a growing number of libraries ...Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized parts ...

... Fluorescent Protein Plasmids , Luciferase Plasmids Viral Plasmids These plasmids are modified viral genomes...Recombinant DNA Working with Plasmids Plasmid Elements Types of Plasmids Molecular Cloning Common E. coli... some codons to encode for synthetic amino acids. Plasmids and Recombinant DNA Technology Chemistry techniques...elements using specifically engineered plasmids. These plasmids have become possibly the most ubiquitous...out our sequencing primers page . Types of Plasmids Plasmids are versatile and can be used in many different...CRISPR Guide . CRISPR Plasmids , Cre-lox and Other Recombinases , TALEN Plasmids and Kits , Genome Engineering...Kits Reporter Plasmids Used for studying the function of genetic elements. These plasmids contain a reporter...

...on different plasmids, the viral genome remains largely intact. First-generation plasmids include (Figure...lentiviral plasmids. Created with BioRender.com. Second-generation Second-generation lentiviral plasmids greatly...in second-generation transfer plasmids as well. Third-generation plasmids include (Figure 4): Transfer ... transfer plasmids can be packaged by either second- or third-generation packaging plasmids. For a summarized...transfer plasmids that have been deposited with Addgene are self-inactivating (SIN) plasmids. These plasmids...included a glossary at the end! Browse lentiviral plasmids available at Addgene. Lentivirus Versus Gamma-...required components have been separated into distinct plasmids for safety. For a summary of all lentiviral plasmid...

...Many of these plasmids also contain one or multiple copies of the cognate tRNA gene. ID Plasmid Synthetase...codons for non-canonical amino acid incorporation. ID Plasmid Gene/Insert Vector Type PI Return to top Bacterial...to enhance non-canonical amino acid incorporation. ID Strain Description PI 48998 C321.ΔA all TAG sites...Find GCE synthetase plasmids, target plasmids, and bacterial strains useful for expanding the genetic...Expansion Genetic Code Expansion Synthetase Plasmids Target Plasmids Bacterial Strains The genetic code for...incorporation of amino acids beyond the 20 standard ones, known as non-canonical amino acids (ncAAs). This strategy...achieve these modifications, two plasmids are generally used. Once these plasmids have been introduced in the...

... Engineered Variants Hybrid capsids Hybrid capsids are engineered capsids derived from multiple different...Examples of AAV serotypes, pseudotypes, hybrid capsids, and capsids created through directed evolution. Created...all of Addgene’s transfer plasmids are replication deficient. These plasmids can be used to create viral...'ve included a glossary at the end! Browse AAV Plasmids Adeno-Associated Virus versus Adenovirus Adenoviruses...plasmid. In order to produce rAAV vectors, three plasmids are needed: Transfer plasmid (also known as cis...necessary for the production and assembly of viral capsids. Helper plasmid — containing adenoviral genes not...see the AAV Plasmid Elements table . Figure 2: Plasmids required for rAAV vector production. Created with...

...on separate plasmids ( in trans ). This method, therefore, reduces the number of plasmids that are required...transfer plasmids that have been deposited with Addgene are self-inactivating (SIN). These plasmids have ...included a glossary at the end! Browse retroviral plasmids available at Addgene. Gamma-Retrovirus Versus ...Gamma-retroviral vectors do not have defined “generations” of plasmids, as lentiviral vectors do. For more about lentiviruses...required components have been separated into distinct plasmids for safety to reduce the chance of creating replication-competent...produce gamma-retroviral vectors, you need three plasmids (Figure 2): Transfer plasmid — contains transgene...(known as tropism). Figure 2: Gamma-retroviral plasmids. Created with BioRender.com. Gamma-Retroviral ...

...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Figure 2: Overview ...later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease...specificity and activity are discovered. Browse Plasmids: Single-Strand Break (Nick) , Double-Strand Break...archaea use to protect against these foreign nucleic acids. Acr family members inhibit CRISPR by a variety ...CRISPR editing only to the target tissue. Browse Plasmids: Anti-CRISPR Small Precision Edits CRISPR can ... (see: Validate genetic modification ). Browse Plasmids: Double-Strand Break (Cut) , Endogenous Tagging...

...of non-recombinant adenoviral plasmids. Recombinant adenoviral plasmids are then verified for size and...structural proteins needed to assemble icosahedral capsids and build new virions. Figure 1: Wild-type adenovirus...included a glossary at the end! Browse Adenoviral Plasmids Adenovirus versus Adeno-Associated Virus Adenoviruses...genes are now supplied in trans , via different plasmids or the packaging cell line, which not only frees...to produce rAdV vectors, you generally need two plasmids: Shuttle plasmid (also known as transfer plasmid...packaging signal from the HV genome or using other plasmids instead of HVs. Figure 4: Third-generation rAdV...enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction sites or may only...