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We narrowed to 809 results for: Eng

Showing: 801 - 809 of 809 results

  1. Adenovirus Guide

    Type
    Guide
    ...inside. Adenoviral genomes range between 26–45 kb in length and contain multiple heavily-spliced transcripts...regions are also provided in trans — that is, by engineered packaging HEK293 cell lines. Second-generation...provide the deleted adenoviral genes. The biggest challenge of the HCAdV vector production is to ensure no...occurs directly in the HEK293 packaging cell line, engineered for maximum adenoviral production, and is driven... protect nonhuman primates against ebolavirus challenge . Journal of Virology, 85 (9), 4222–4233. https..., R., Yu, X., Farina, E. M., Yan, S., Zhao, C., Zheng, Z., Shu, Y., Wu, X., Lei, J., Li, Y., Zhang, W....: 31659156 (Link opens in a new window) Luo, J., Deng, Z. L., Luo, X., Tang, N., Song, W. X., Chen, J....

  2. Lentiviral Vector Guide

    Type
    Guide
    ...for decades. Wild-type lentiviruses have been engineered into lentiviral vectors that can be safely used...used in laboratory settings. These engineered lentiviral vectors have many aspects that make them useful...transcription, inhibiting transcription of the full-length virus after it is incorporated into a host genome...HEK293T cell lines that have been specifically engineered for lentiviral production. All lentiviral plasmids...deletion prevents further transcription of the full-length virus. The potential for oncogenesis is largely...Lentiviral Vector Uses and Overview Overcoming the Challenges of Lentiviral Production Your Lentiviral Plasmid...deliver genetic material into different host cells. Engineered for safety and efficiency. Content last reviewed...

  3. Antibody Guide

    Type
    Guide
    ...emit a specific wavelength of light after excitation by light of a specific wavelength. While more expensive...monovalent nature. Darpins - Darpins are genetically engineered small proteins derived from ankyrin repeat proteins... constant region. Minibodies - Minibodies are engineered antibody fragments consisting of two scFvs bound... in a range of colors activated by different wavelengths, allowing for easy differentiation between multiple...recognizes and binds to the protein of interest. Signal strength is determined by how many antibodies bind the ... break DNA up into fragments of 300-1000 bps in length. Note: This sonication process may need to be optimized...

  4. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...decades. Wildtype gamma-retroviruses have been engineered into gamma-retroviral vectors that can be safely...safely used in laboratory settings. These engineered gamma-retroviral vectors have many aspects that make...lines (Figure 3B). These cell lines have been engineered to stably express gag-pol and/or env , eliminating...deletion prevents further transcription of the full-length virus. The potential for oncogenesis is largely...Bosch, V., Nocken, F., Merget-Millitzer, H., Engelstädter, M., Kurth, R., Groner, B., & Cichutek, K. (...deliver genetic material into different host cells. Engineered for safety and efficiency. Content last reviewed...

  5. Plan Your Experiment

    Type
    Guide
    ...generally higher than HDR pegRNA optimization can be challenging Prime Edit Repress or Interfere (CRISPRi) Reduce...them. To increase specificity, consider using an engineered Cas enzyme with higher fidelity. In addition ...gRNAs that have been used successfully in genome engineering experiments. Using validated gRNAs can save your...References Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9 system. Methods in Molecular...Glass, Z., Lee, M., Li, Y., & Xu, Q. (2018). Engineering the delivery system for CRISPR-Based genome editing...

  6. Science Guides

    Type
    Guide
    ...Chemogenetics is a technique that uses genetically engineered receptors to selectively control and investigate...system in bacteria, have been modified for genome engineering. Find essential background information on CRISPR...Optogenetics Optogenetics integrates optics and genetic engineering to measure and manipulate cells (frequently neurons...

  7. Molecular Cloning Techniques

    Type
    Guide
    ... orientation within its MCS. Due to their short length, it is also easy to add restriction sites to any...Choosing Your Perfect Plasmid Backbone Genome Eengineering Guide Addgene Protocols Plasmid cloning by restriction...

  8. Educational Resources

    Type
    Guide
    ...molecular biology techniques. Topics include: Genome Engineering Fluorescent Proteins CRISPR Viral Vectors Plasmid...

  9. Promoters

    Type
    Guide
    ...into a protein. RNA polymerase II — transcribes messenger RNA (mRNA), which is the RNA responsible for providing...
Showing: 801 - 809 of 809 results