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Showing: 801 - 820 of 879 results

  1. Adenovirus Guide

    Type
    Guide
    ...of non-recombinant adenoviral plasmids. Recombinant adenoviral plasmids are then verified for size and...structural proteins needed to assemble icosahedral capsids and build new virions. Figure 1: Wild-type adenovirus...included a glossary at the end! Browse adenoviral plasmids available at Addgene. Adenovirus versus Adeno-...genes are now supplied in trans , via different plasmids or the packaging cell line, which not only frees...to produce rAdV vectors, you generally need two plasmids: Shuttle plasmid (also known as transfer plasmid...packaging signal from the HV genome or using other plasmids instead of HVs. Figure 4: Third-generation rAdV...enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction sites or may only...

  2. Molecular Cloning Techniques

    Type
    Guide
    ... Addgene Blogs Plasmids 101: Restriction Cloning Plasmids 101: Gateway Cloning Plasmids 101: TOPO Cloning...entry plasmids are also available, allowing for rapid cloning of PCR products into donor plasmids without...Cloning Plasmids 101: Gibson Assembly Plasmids 101: Golden Gate Cloning Tips and Tricks for Using Golden...Modular Cloning Plasmids 101: Sequence and Ligation Independent Cloning (SLIC) More Plasmids 101 topics More...manipulate genes of interest and then insert them into plasmids for replication and protein expression. There ...fragments. Given its prevalence, the vast majority of plasmids used for DNA cloning and expression contain several... becomes progressively more difficult to clone plasmids larger than 10 kb. Another advantage is the ability...

  3. Guide to Using Pooled Libraries

    Type
    Guide
    ...are single preparations of many different plasmids. Plasmids within a given library have the same backbone...the overall library. Barcoding libraries contain plasmids with unique, semi-random sequences that can be...libraries are supplied as a mixed population of plasmids in a single tube. Pooled libraries can be small...lab has over 175,000 different gRNA-containing plasmids). Amplifying and Using the Library Once you receive...notably pooled lentiviral plasmid libraries, the plasmids must first be used to make virus. This pooled ...pooled virus is subsequently used to deliver the plasmids to the cells of interest. In either case, next-generation...need to infect many more cells than the number of plasmids present in the library - for example, the Moffat...

  4. Optogenetics Guide

    Type
    Guide
    ...Addgene's guide to using Optogenetics plasmids in your lab, including explanations of microbial opsin...Biosensors Plasmid Collection . To find optogenetics plasmids available through Addgene, check out our Optogenetics... of opsins here to find the right optogenetics plasmids for your experiments. Channelrhodopsins Channelrhodopsins...SwiChRca, Phobos, Aurora Browse Channelrhodospin plasmids . Halorhodopsins Halorhodopsins are light-gated...action spectra Example: Jaws Browse Halorhodopsin plasmids . Archaerhodopsins Archaerhodopsin-3 (Arch) from...Examples: eArch3.0, eArchT3.0 Browse Archaerhodopsin plasmids . Wild-Type Mac Leptosphaeria rhodopsin (Mac) ...amplitude Example: eMac3.0 Browse Leptosphaeria plasmids . Glossary of Microbial Opsin Variants Opsin Type...

  5. TALEN Plasmids and Kits

    Type
    Collection
    ...Takashi Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI ...and accessory plasmids available from Addgene for generating your own specific TALE plasmids. Genome...TALEN Plasmids and Kits You may also like... Addgene's Genome Engineering Guide CRISPR Plasmids Zinc Finger...TALE Toolbox 49622 - 49647 24 plasmids Thoru Pederson TALEColor plasmids: destination vectors for use ...Musunuru/Cowan Lab TALEN Kit Kit comprised of 834 plasmids of pre-assembled trimer and tetramer RVD domains... 38142 RCIscript-GoldyTALEN Daniel Carlson The plasmids pC-GoldyTALEN and RCIsricpt-GoldyTALEN are designed...Golden Gate TALEN 2.0 36033 pTAL5-BB Tom Ellis Plasmids pTAL5-BB and pTAL6-BB are designed as alternative...

  6. Plan Your Experiment

    Type
    Guide
    ...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells..., anneal, and insert the targeting oligos into plasmids containing the gRNA scaffold using standard restriction-ligation...fluorophore) are also relevant. Delivery Using Plasmids The most straightforward method to deliver a CRISPR...mammalian cells is to directly transfect expression plasmids encoding your Cas protein and gRNA, which you ...options. Browse Addgene's collection of CRISPR plasmids . Delivery Using Viral Vectors Viral vectors are...You then delivery the RNP to cells using cationic lipids, electroporation, or an endosomal approach. Similar...

  7. Promoters

    Type
    Guide
    ...Expression Plasmids 101: The Promoter Region — Let's Go! Plasmids 101: Inducible Promoters Plasmids 101: Repressible...Repressible Promoters Plasmids 101: Terminators and PolyA signals More Plasmids 101 topics Content last...often incorporate the following promoters into plasmids to drive constitutive or inducible expression....molecules that are responsible for bringing amino acids to the ribosome when proteins are being synthesized...

  8. Antibody Guide

    Type
    Guide
    ...little variation. Recombinant plasmids - Antibodies can be produced from plasmids, with an antibody’s genetic... genes from specific hybridoma cells into plasmids. Plasmids are then transfected into cells and induced...immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals make... ready-to-use recombinant antibodies made from plasmids in our collection. View Antibodies...

  9. Control AAV Preps

    Type
    Collection
    ...VCre-dependent Constitutive (non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI...We provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection...regulatory dTomato Constitutive 1, 9, PHP.eB Jordane Dimidschstein 135631 pAAV-S5E2-GFP-fGFP E2 regulatory EGFP...EGFP Constitutive 1, 9, PHP.eB Jordane Dimidschstein 135641 pAAV-S5E6-dTom-nlsdTom E2 regulatory dTomato...dTomato Constitutive 1 Jordane Dimidschstein 137135 pAAV-Ef1a-oScarlet EF1a oScarlet Constitutive 8 Karl Deisseroth...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

  10. Addgene's eBook Collection

    Type
    Guide
    ...Selected Download Select Plasmids 101 September 2023 | Fourth Edition About Plasmids 101 September 2023 ...popular Plasmids 101 blog series, this eBook covers everything you need to know about plasmids, from parts...

  11. Educational Resources

    Type
    Guide
    ...topics related to our collections. Titles include: Plasmids 101 CRISPR 101 Viral Vectors 101 Fluorescent Proteins...include: Protocols Careers How-to Blog Read about plasmids in Addgene’s repository , and related molecular...Career Readiness Molecular Biology Learn about plasmids, sequencing, vectors, and restriction enzymes ...

  12. Chemogenetics Guide

    Type
    Guide
    ...Addgene's guide to using Chemogenetics plasmids in your lab for interrogation of neuronal activity.... ready-to-use AAV preparations of chemogenetic plasmids . Additionally, genetically engineered animal ...available. Table 4: Common promoters in chemogenetics plasmids Promoter Cell Specificity hSyn1, CaMKIIa Neurons...

  13. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Lab Improved Phosphoprotein Synthesis Reagents Type ID Item Strain 192872 rEcoli XpS (MG1655-C321 mutS+,... other plasmids containing high copy ColE1/PUC-like origins. Therefore, when choosing plasmids to express... central phosphoserine site flanked by 15 amino acids on either side occurring within the parent protein...

  14. Science Guides

    Type
    Guide
    ...are single preparations of many different plasmids. Plasmids within a given library have the same backbone...

  15. Sequencing Primers

    Type
    Guide
    ...For additional information on molecular biology, plasmids, and recombinant DNA, please consult Addgene's...GCAACGTGCTGGTTATTGTG Rabbit beta-globin intron, for pCAG plasmids Forward pCasper-F GGGTTTTATTAACTTACAT 5' end of...

  16. Rett Syndrome

    Type
    Collection
    ...bacterial) Insert species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return...Rudolf Jaenisch labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR...Broswe plasmids and other research tools for use in Rett Syndrome research. Plasmid...MECP2 Animal Models Human Cell Lines CRISPR Tools Plasmids Resources The (Link opens in a new window) Rett...mammalian CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few...gene. Use the search bar or sort buttons to find plasmids based on: Expression system (mammalian or bacterial...

  17. Optogenetics AAV Preps

    Type
    Collection
    ...AAV1 AAV2 AAV5 AAV8 AAV9 AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype...We provide high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV...electrical potentials in neurons. See our Optogenetics Plasmids and Optogenetics Guide page for more information... ready-to-use AAV preparations of optogenetics plasmids available in Addgene's collection. Opsin Excitatory...ChR2 mCherry Constitutive 1, 9, PHPeB Jordane Dimidschstein 137142 pAAV-nEF-Con/Fon-ChR2-mCherry nEF ChR2...C1V1 EYFP Constitutive 1, 5, 9, PHPeB Jordane Dimidschstein 100061 pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry EF1a...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

  18. Retrograde AAV viral preps

    Type
    Collection
    ...Activators Inhibitors Other Molecular Tools Clear Filters ID Name Promoter Activity Category PI 37825 AAV-CAG-...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

  19. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Subpool Viral Prep Available Yes No Clear Filters Name ID Library Type Species PI Lentiviral Generation gRNAs...Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick ...CRISPR pooled libraries consist of thousands of plasmids, each containing multiple gRNAs for each ...

  20. Biosensor AAV Preps

    Type
    Collection
    ...AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Promoter Sensor Label Activity Serotype PI Acetylcholine...GCaMP6f E2 GCaMP6f none Constitutive 1, 9, PHPeB Dimidschstein 137122 pAAV-Ef1a-Con/Fon-GCaMP6F EF1a GCaMP6f...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....
Showing: 801 - 820 of 879 results