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We narrowed to 1,073 results for: TOR

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Showing: 901 - 920 of 1073 results

  1. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg et al., 2014...conformational changes that make Cas9 such a valuable laboratory tool are still an area of active research. New...

  2. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...expected location of the target is an important factor in deciding which protocol is likely to work, we...more permanent knockdown, consider using shRNA vectors instead. With both siRNA and shRNA, it can be difficult...

  3. Browse

    Type
    Collection
    ...Consortium Plasmids All SGC Plasmids Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry PI...

  4. The SGC Oxford Collection

    Type
    Collection
    ...Consortium Plasmids - Oxford Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...

  5. The SGC Stockholm Collection

    Type
    Collection
    ...Consortium Plasmids - Stockholm Plasmid Gene/Insert Vector Type Mutations Tags Industry PI...

  6. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...plasmid Cut vector - Background due to uncut vector Cut vector + Background due to vector re-circularization...ends on either side of the vector are compatible with each other, the vector is much more likely to ligate...fuse the two ends of the vector together). Protocol: Standard Insert + Vector DNA Ligation Before setting...amount of cut insert and vector to use for the ligation reaction. The volume of vector DNA and insert DNA used...where the insert is smaller than the vector) a 3 insert : 1 vector molar ratio will work just fine. We ...opens in a new window) ligation calculator to easily quantify how much vector and insert DNA to use. Combine... ALWAYS do a vector alone + ligase control. This will allow you to verify that the vector was completely...

  7. Synthetic Biology - Strains

    Type
    Collection
    ...link to view all plasmids available from this depositor's lab. Plasmid Description Gene/Insert Antibiotic...

  8. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Xenopus". Cut ID...

  9. TREAT-AD Plasmid Collection

    Type
    Collection
    ..., gene/insert, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...

  10. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "C. elegans" or "...

  11. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Drosophila". Activate...

  12. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species Mammalian Bacteria Drosophila Plant ...

  14. CRISPR Plasmids - Plants

    Type
    Collection
    ...plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Plant". Activate...

  15. CRISPR Plasmids - Yeast

    Type
    Collection
    ...plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Yeast". Activate...

  18. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Bacteria". Activate...

  19. CRISPR Plasmids - Parasites

    Type
    Collection
    ...plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Parasite". The content...
Showing: 901 - 920 of 1073 results