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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 906 results for: nes

Showing: 901 - 906 of 906 results

  1. Plan Your Experiment

    Type
    Guide
    ...more effective than dCas9 alone for mammalian cell lines Interfere Activate (CRISPRa) Increase gene expression...can be transient, or you can generate stable cell lines if your plasmid contains a selection marker. Plasmid..., note that this method is most useful for cell lines that you can transfect at high efficiency, such ... are a popular option for producing stable cell lines, since the genetic cargo (in this case, CRISPR components...is a common choice for generating modified mouse lines. Protein delivery Lastly, you can deliver Cas-gRNA...Elongation roadblocks mediated by dCas9 across human genes modulate transcription and nascent RNA processing...

  2. Chemogenetics Guide

    Type
    Guide
    ... PSAM plasmids ! LMOs Luminopsins (LMOs), or luminescent opsins, are opto-chemogenetic fusion proteins... LMOs a versatile option. Coupling brighter bioluminescent modules, such as luciferase-fluorescent protein... a single molecule driven by FRET-modified bioluminescence. Neurophotonics. 11:021005. PMID: 38450294 ...Manipulation for Ameliorating Huntington's Disease Pathogenesis. iScience , in press. Magnus CJ, Lee PH, Atasoy...Inhibitory luminopsins: genetically-encoded bioluminescent opsins for versatile, scalable, and hardware-independent...

  3. Optogenetics Guide

    Type
    Guide
    ... chimera 470 ChIEF, ChEF, ChD Engineered by chimeragenesis of ChR1 and ChR2 450-470 C1V1 ChR1-VChR1 chimera...injection site. Optical Switches Researchers have harnessed plant and bacterial photoreceptors to create protein...improve upon previous tools through directed mutagenesis - use Addgene's Optical Switch Plasmid Table ... sequester and release proteins from cellular membranes using light. light-induced protein dissociation...

  4. Cloning

    Type
    Guide
    ...molecular cloning scientists can amplify and manipulate genes of interest and then insert them into plasmids for...putting it under a variety of promoters and into backbones with different selection cassettes). Although ...disadvantage of TOPO® cloning is that very few plasmid backbones are available TOPO® ready, and it is not feasible...

  5. Educational Resources

    Type
    Guide
    ...CRISPR Viral Vectors Plasmid Technology Career Readiness Molecular Biology Learn about plasmids, sequencing...

  6. Science Guides

    Type
    Guide
    ... backbone, but they express or target different genes. Some libraries cover the majority of the genome...
Showing: 901 - 906 of 906 results