We narrowed to 1,041 results for: ins
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TypeBlog Post...They all found the support of the group to be intrinsic to their success. Their stories made it clear ...
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Celebrating 15 Years of Scientific Sharing
TypeBlog Post...up in both science journals like Nature and in mainstream media like The Atlantic. We’ve been in documentaries... -
Human Germline Editing Using CRISPR
TypeBlog Post...oligonucleotide (ssODN) template supplied by Ma et al., but instead from the mother’s wild type allele (Figure 1). ... -
Summer SciComm: Preprints
TypeBlog Post...scientists can receive recognition for their efforts, inspire future research, and increase their impact. Open... -
Transferable Skills Guide: Public Speaking
TypeBlog Post... with very little text on the slides and notes instead of written speeches. When you’re doing all that... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog Post...addition to the 20 bp guide sequence, the crRNA also contains a ~20 bp repeat region on its 3’ end. This repeat... -
Transferable Skills Guide: Cross-team Communication
TypeBlog Post... Eric J. Perkins... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...Novartis Oncology. Bhang did her PhD work at Johns Hopkins and came to Novartis for her post-doc in January... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog Post...seriously. As I make my rounds speaking at research institutions and at scientific conferences, I see that few... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...certain cell types. Botond Roska’s lab at the Institute of Molecular and Clinical Ophthalmology Basel ... -
Engaging with science and society at pgEd
TypeBlog Post...organizations, libraries, youth groups, and faith institutions. The conversations often move quickly into sensitive... -
Rabies and Neuronal Tracing
TypeBlog Post...since these processes are mediated by other viral proteins), but the newly released RABV cannot infect any... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post...The day has arrived; you’ve painstakingly cared for your packaging cell line, prepped your DNA, transfected... -
Genomic Deletions in Mammalian Cell Lines
TypeCollection...ml). NOTE: Phosphorylated oligos may be ordered instead. For this approach, the use of T4 PNK is omitted...type. While MEL cells are suspension cells, instructions for adherent cells have also been included. ... clones to a single 96-well plate that already contains 50 μl cell culture media per well for a final ...protein, perform an immunoblot using an antibody against the relevant protein 9 . References This protocol... -
Academic vs. Industry Postdocs
TypeBlog Post... decide what they’d like to work on later. For instance, AstraZeneca posts most of its positions all at... -
Neuroscience
TypeCollection...recombinant antibodies targeting neuroscience-related proteins....currently available from our catalog that target proteins popular in neuroscience research. These ready-... represent the official views of the National Institutes of Health.... -
CRISPR Plasmids - dCas9-FokI
TypeCollection...Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication...Publication Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids... -
Michael Davidson Fluorescent Protein Collection
TypeCollection... Plasmid Collections Fluorescent Proteins Michael Davidson Fluorescent Protein Collection...collection of ORFs tagged with a variety of fluorescent proteins. The majority of the ORFs are of mammalian origin...different fluorescent tags for you to create fusion proteins with your gene of interest. Please visit Michael... -
CRISPR Plasmids - RNA Targeting
TypeCollection...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert PI Publication...Publication Plant ID Plasmid Gene/Insert PI Publication Do you have suggestions for other plasmids that should... -
CRISPR Plasmids - Xenopus
TypeCollection...joining (NHEJ); a mechanism which frequently causes insertions or deletions (InDels) in the DNA, possibly resulting... with the Cas9 and gRNA plasmids, the cell may instead repair the break using homology-directed repair...introduce specific genomic changes. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors...