Addgene

We narrowed to 950 results for: APP

Showing: 941 - 950 of 950 results

Results per page:

Protocol - Over-Agar Antibiotic Plating

Type

Protocol

...over-agar Plate shows a lawn of E. coli and no apparent selection. 150 µL of 1 mg/mL Carbenicillin plated...
Plasmid Modification by Annealed Oligo Cloning (with Protocols)

Type

Protocol

...years to come. Design Briefly, we will design overlapping oligos that once annealed can be cloned directly...
Fluorescence Titering Assay

Type

Protocol

... these cells can still be used in downstream applications. Safety Warnings Lentivirus is generally considered...
General Transfection

Type

Protocol

...), but any HEK293T cell line should work. This approach can be adapted for different cell lines and different...
Transfection for Recombinant Antibodies

Type

Protocol

...antibody can be purified for use in a variety of applications. Sharing speeds science. We believe that sharing...
What is Polymerase Chain Reaction (PCR)

Type

Protocol

...amplified. When these reagents are combined in an appropriate buffer, a series of heating (denaturing) and ...
AAV Titration by qPCR Using SYBR Green Technology

Type

Protocol

...~3.3 difference Ct for a 10-fold dilution is appropriate). Quality of duplicates: Exclude duplicates from...
Plasmid Cloning by PCR (with Protocols)

Type

Protocol

...want a recipient plasmid to insert ratio of approximately 1:3. Since the number of base pairs for each...
Antibody Validation Using the Indirect ELISA Method

Type

Protocol

...add 100 µL of primary antibody solution to the appropriate wells. Cover the plate with a plate seal and ...
AAV Production in HEK293 Cells

Type

Protocol

...incubator for ~72 h. Harvest cells and media by tapping the sides of the CS5. Cells should detach easily...

Showing: 941 - 950 of 950 results

Results per page:

Protocol - Over-Agar Antibiotic Plating

Plasmid Modification by Annealed Oligo Cloning (with Protocols)

Fluorescence Titering Assay

General Transfection

Transfection for Recombinant Antibodies

What is Polymerase Chain Reaction (PCR)

AAV Titration by qPCR Using SYBR Green Technology

Plasmid Cloning by PCR (with Protocols)

Antibody Validation Using the Indirect ELISA Method

AAV Production in HEK293 Cells

Help Center

Deposit Plasmids

General Reagents

Viral Service

DNA Service

Plasmid Collections