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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 994 results for: Ran

Showing: 961 - 980 of 994 results

  1. AAV ddPCR Titration

    Type
    Protocol
    ...outlined in this protocol are based on an AAV titer range of 5E+12–5E+13 GC/mL, where GC is genome copies ...the DG8 gasket, making sure that it is secure. Transfer the cartridge holder to the droplet generator....while slowly and gently aspirating the droplets. Transfer the droplets to a prechilled PCR plate. Pro-Tip... 98 10 2 1 Hold 4 ∞ 2 1 After PCR is complete, transfer the plate to the Droplet Reader. Open the QuantaSoft...

  2. Protocol - How to Design Primers

    Type
    Protocol
    ...complementary to template strand). However, primers do not need to correspond to the template strand completely; it...primer corresponds completely to the template DNA strand so elongation can proceed. Usually a guanine or...

  3. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ... of Plasmids You may also like... Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture... not need to obtain more competent cells and retransform. Bacteria on an LB agar plate can be stored at...frozen bacteria, preventing damage to the cell membranes and keeping the cells alive. A glycerol stock ...

  4. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ... Inclusion of this information is solely for transparency intended to support reproducibility in science...case, use lower antibody concentrations. The ideal range of the standard curve will vary between targets ...your unknown sample’s absorbance falls above the range of the standard curve you will need to either include...

  5. Kit Free RNA Extraction

    Type
    Protocol
    ...cells. Extract RNA from the homogenized sample(s).Transfer tissue/cell lysate to a 4 mL tube. Add the following...the cold room may damage it. Using a pipettor, transfer the top, aqueous phase to a new RNAse-free tube...

  6. DNA Quantification

    Type
    Protocol
    ...spectrophotometer. A spectrophotometer uses the absorbance/transmission of light through a liquid to determine the concentration...measured under the 260/280 column (A good purity ranges from 1.80-2.00). Repeat for each sample. Notes:...

  8. Using a Light Microscope Protocol

    Type
    Protocol
    ...your cell culture dish. Microscopes come in a huge range of shapes and sizes - from phone-sized, foldable...powerful (but are cheap and accessible) to massive transmission electron microscopes that allow us to see cellular...

  9. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...the solution has a tendancy to boil over. Placing saran wrap over the top of the flask can help with this...are usually referred to as ‘bands’ due to their appearance on the gel. Pro-Tip If you will be purifying ...

  10. Pouring LB Agar Plates

    Type
    Protocol
    ...x (0.220 L) = 8.14 g pre-mixed LB-agar powder. Transfer the LB-agar powder you’ve measured out into an...molten agar from boiling over in the autoclave. Transfer the sterile water (in our case 220 mL) to the ...

  11. CRISPR Guide

    Type
    Guide
    ..., 1163–1171. PMID: 23979020 Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D...38688943 Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine..., 17 (5), 471–479. PMID: 32203383 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I....CRISPR-Cas9. Cell , 167 (7), 1829-1838.e9. PMID: 27984730 Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott...Nature Protocols , 8 (11), 2281–2308. PMID: 24157548 Ran, F. A., Cong, L., Yan, W. X., Scott, D. A., Gootenberg...may be a single-stranded oligonucleotide, double-stranded linear DNA, or a double-stranded DNA plasmid. ...including transposons, integrases, and recombinases, with Cas enzymes. CRISPR Transposases Transposon systems...

  12. Protocol - How to Streak a Plate

    Type
    Protocol
    ...also like... Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture...

  15. Centrifugation

    Type
    Protocol
    ... tubes. Depending on the centrifuge their speed ranges can vary, but are typically lower than some other...

  17. Pipetting Protocol

    Type
    Protocol
    ...boxes that the tips come in often indicate a volume range that the tip can hold. This should give you an idea...

  19. Lentiviral Vector Guide

    Type
    Guide
    ...deletion is transferred to the 5’ LTR after one round of reverse transcription, inhibiting transcription of the...cannot be used in direct transfections for transient expression of a transgene. For more detailed protocols... Increases transduction efficiency and transgene expression. Packaging plasmid gag in trans Precursor ...packaging plasmids. tat in trans Trans-activator; binds TAR to activate transcription from the LTR promoter...crossover event between the transfer plasmid and genes provided in trans . Transduction The process of artificially... typically ranges from 8–10 kb, encoded on RNA (Figure 1). This RNA is reverse transcribed into the provirus...for third generation) plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked...

  20. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...double-stranded DNA genome. Adeno-associated viruses (AAV), however, are small single-stranded DNA viruses...plasmids are needed: Transfer plasmid (also known as cis plasmid) — containing the transgene of interest between...Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid can be done with...three production plasmids (transfer, packaging, and helper plasmid) are transfected into cells which then ... to display neuronal retrograde transport, AAVrg. Retrograde transport refers to the direction of movement...machinery to synthesize the complementary strand, transgene expression may be delayed. To overcome this...express a viral element necessary for RABV trans-synaptic transport. Learn more about these and other initiatives...
Showing: 961 - 980 of 994 results