We narrowed to 1,050 results for: ins
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TypeCollection...Collection Allen Institute for Brain Science AAV Enhancer Collection Allen Institute for Cell Science ...Collections Depositor Collections Addgene's repository contains 165,075 plasmids, which includes plasmid collections...Reagents for improved expression of recombinant phosphoproteins - Rinehart FreeGenes Project Open Enzyme Collection...
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CRISPR Plasmids - Purify Genomic Loci
TypeCollection...Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter...subsequently isolated by affinity purification against the epitope tag. Compared to conventional methods...Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return... -
AAV Viral Preps
TypeCollection...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre...Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti Monster, Brainbow Molecular Tools ... -
CRISPR Plasmids for Genomic Visualization
TypeCollection...recruit specific RNA-binding proteins (RBPs) tagged with fluorescent proteins to visualize targeted genomic...requires recruitment of many copies of labeled proteins to the given region. For example, chromosome-specific...read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return... -
CRISPR Plasmids - Parasites
TypeCollection...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma...knockout gRNA pooled library designed with 10 guides against 8,158 predicted protein-coding genes. Do you have... -
CRISPR Plasmids - C. elegans
TypeCollection...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically ...activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors... -
Plasmids for Stem Cell Research
TypeCollection... L-Myc, Lin28, OCT3/4, and shRNA against p53 in different gene/insert combinations A more efficient method...regenerative medicine. iPSCs, much like their ES cell cousins, have the hallmarks of pluripotent cells--they ...Ottosson Stem Cell Research Plasmids This table contains a general list of plasmids that have been used...name, species, gene name, and more. Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions... -
TALEN Guide
TypeCollection...for targeting enzymes and other useful protein domains to a specific DNA sequence ( Figure 1A ). Scientists...consisting of 12-31 repeats can be assembled and inserted into a variety of backbones in just a few steps...Toolbox", from Dr. Feng Zhang’s group at the Broad Institute, will be available from Addgene before the end...Pubmed PMID: 21822241 . TAL effectors: customizable proteins for DNA targeting Bogdanove AJ, Voytas DF. Science... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection...drug-inducible approach may also be used. Split proteins: The recombinase is split into inactive N- and... repetitive recombination leading to genomic instability and cell death. It is important to incorporate...Recombinase Plasmids ID Plasmid Description Gene/Insert Vector Type Promoter PI Return to top Recombinase-Dependent...making transgenic mice. ID Plasmid Description Gene/Insert Vector Type PI Pooled Libraries Pooled Library ... -
Optogenetics AAV Preps
TypeCollection...optogenetics AAV collection, including channelrhodopsins, halorhodopsins, and more....Inhibitory Arch and variants eNpHR and variants InSynC (non-opsin) Jaws WiChR stGtACR iC++ OPN3 PdCO PPO...Edward Boyden 50972 AAV-SYP1-miniSOG-T2A-mCherry Syn InSynC (non-opsin) mCherry Constitutive 8 Roger Tsien ... -
Neuroscience
TypeCollection...recombinant antibodies targeting neuroscience-related proteins....currently available from our catalog that target proteins popular in neuroscience research. These ready-... represent the official views of the National Institutes of Health.... -
CRISPR Plasmids - dCas9-FokI
TypeCollection...Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication...Publication Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids... -
Michael Davidson Fluorescent Protein Collection
TypeCollection... Plasmid Collections Fluorescent Proteins Michael Davidson Fluorescent Protein Collection...collection of ORFs tagged with a variety of fluorescent proteins. The majority of the ORFs are of mammalian origin...different fluorescent tags for you to create fusion proteins with your gene of interest. Most of these plasmids... -
CRISPR Plasmids - RNA Targeting
TypeCollection...Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert PI ...Publication Return to top Plant ID Plasmid Gene/Insert PI Publication Return to top CRISPR Resources Addgene... -
CRISPR Plasmids - Xenopus
TypeCollection...joining (NHEJ); a mechanism which frequently causes insertions or deletions (InDels) in the DNA, possibly resulting... with the Cas9 and gRNA plasmids, the cell may instead repair the break using homology-directed repair...introduce specific genomic changes. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors... -
Structural Genomics Consortium Plasmids
TypeCollection... determine the three dimensional structures of proteins of medical relevance, and place them in the public...pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine tag and TEV cleavage...pFHMSP-LIC-C N/A Expression vector for secreted proteins with C-terminal hexahistidine tag and TEV cleavage... -
CRISPR Plasmids - Zebrafish
TypeCollection...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Vector Type Promoter PI Publication Empty gRNA ... -
CRISPR Plasmids - gRNAs
TypeCollection...recommended that you determine if your cell type contains the target sequence before using any of these ...single species or PAM binding variant of Cas9. For instance, wild-type SpCas9 must be used with targets that...experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions... -
CRISPR Plasmids and Resources
TypeCollection...generates large genomic deletions. Insert NEW CRISPR transposases insert large fragments of DNA. Transcriptional...nickase enzymes fused to reverse transcriptase install new genetic information into a specific DNA site... -
p53 Pathway
TypeCollection...candidate Scotin Shisa family member 5 Sestrins SESN1 SESN2 SESN3 Sestrins 1, 2, or 3 Siah Siah E3 ubiquitin...DNA-damage-inducible; alpha, beta, or gamma IGF-BP3 Insulin-like growth factor binding protein 3 KAI CD82 molecule...