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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 402 results for: CAPS;

Showing: 161 - 200 of 402 results

  1. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ... Primary cells recapitulate the natural biology of a cell type of interest better than immortalized lines...to a protein of interest thereby giving it the capacity to enter cells. One such CPP was discovered in...

  2. Clear and Accessible Writing

    Type
    Blog Post
    ...densities are captured at the 17%, 25%, and 60% layers, and genome-containing virions are captured at the 40%...

  4. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...the viral titer (the number of viral particles capable of infecting a host cell) can be tuned so that ...Viral vector platforms within the gene therapy landscape. Sig Transduct Target Ther 6, 53 (2021). DOI: ...

  5. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...Expression Infects Non-Dividing Cells Large Packaging Capacity (>8kb) High Transduction Efficiency Transient...Mouse Cells and Hematopoietic Cells Low Packaging Capacity (~4.5kb From basic science to clinical applications...

  10. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...fluorescent in situ hybridization (FISH) and chromosome capture suffer from low resolution and can’t be used in...editing, but it retains its gRNA-directed targeting capacity. By using a gRNA to target fluorescently labeled...

  11. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently been made...Cas7 and Cas11 domains. Cas7-11 cuts ssRNA and is capable of pre-crRNA self-processing. Functionally, it ...

  14. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...). Bacteria need special fertility genes to be capable of conjugation, and these are encoded by certain...conserve energy, these specialized degradative capabilities are often tightly regulated to be active only...

  16. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...you’re simply attending, but uncertain how to capitalize on your experience. No matter the reason for ...bullet points and elaborate if your audience seems captivated. Expertly navigate questions Answering questions...

  17. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...thin sheet to illuminate a fluorescent sample and captured the reflected light using a different objective...Ultramicroscope was developed using a stereomicroscope to capture large field-of-views (6). For more comprehensive...

  18. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...likely do! Give our summary table a look for a quick recap of the pros and cons of each antibiotic and happy...novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins ...

  19. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...would permit point mutations in RNA which could recapitulate or rescue known pathogenic alleles, or introduce...REPAIRv1 to merely 20. Expanding RNA Editing Capabilities through RESCUE Two years later, the Zhang lab...

  21. DNA Purification Without a Kit

    Type
    Blog Post
    ...hydrophobic glass filter. Each glass filter can capture up to 150 ug of DNA, and filters can be stacked...stacked together in series to increase binding capacity. Figure 1: Purify DNA using glass filters. Image...

  22. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...Advantages of lentivirus include a large genetic capacity and the ability to transduce both dividing and...for applications that require the large genetic capacity of a lentiviral vector but for which transient...

  24. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    .... Since target specificity is determined by the capsid of the AAV, many of the vectors in Addgene’s catalog...including the retrograde AAVrg and the systemic capsid PHP.eB.   Figure 4: A) Cumulative AAV preps...

  25. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...integration of cargo without using screening markers. Capability to construct a strain library with different ... that same year. Her research is focused on the escape mechanism of genome editing in microorganisms which...

  26. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...areas associated) compared to an animal running to escape a threat. Locomotion can thus be used as a readout...behaviors are possible: active swimming in attempts to escape, or passive floating on the water surface. The ...

  29. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...variability, and in some cases, counter-selection escape can occur: single crossover colonies can acquire... the toxin itself (leading to counter-selection escape). The rhamnose induction system is well-characterized...

  30. Five Popular Model Organisms

    Type
    Blog Post
    ...study diseases such as cancer since mice better recapitulate the complex interactions between cancer cells...studied in other model organisms that better recapitulate in vivo conditions similar to humans. For example...

  31. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...with streptavidin, the lateral flow strip first captures the streptavidin portion of the probe via biotin...probe that is cut will continue to flow and be captured by an antibody further along on the strip. Highlights...

  32. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...major equipment can be defined as equipment with capital costs greater than $5,000 but this can depend on...like pipets, or mini-centrifuges, etc. Be sure to capture as many of these tools as possible, this even includes...

  33. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...refers to the different protein compositions of AAV capsids, which determine how a virus enters the cell. Different...Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve Transduction and Volumetric Spread in the...

  34. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...biotech industry, which is in the process of capitalizing on phage RBPs as diagnostic tools and therapeutics...between 1 and 3 kb results in optimal likelihood of capturing a whole RBP gene without causing issues associated...

  35. Weighing Reagents Protocol

    Type
    Protocol
    ...Procedure The balance in the photo above has a capacity of 200 g, as indicated by the number above the...is best. Check that the balance has the correct capacity for the material that you are weighing by looking... you’re weighing out an amount larger than the capacity of your balance or weighing boat, you can weigh...

  36. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...after cleavage by the transposase. It has a cargo capacity >100 kB, although integration efficiency decreases...without decreased efficiency, with a maximum cargo capacity of ~200 kB of DNA. Similar to piggyBac, Tol2 also...

  38. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ... cassettes, making them advantageous for small capacity vectors like AAV. In addition to the mammalian...Yang. “Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.” ...

  39. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...against common tumor markers to enhance cancer cell capture. Fierer et al., PNAS 2014 Apr 1;111(13):E1176...associated with high levels of shRNA expression. The capability to turn RUSH and CRUSH alleles off or on enables...

  40. Video Library

    Type
    Protocol
    ...Addgene's video content, including how-to screen captures, lab procedure protocols, and career advice videos...standard laboratory procedures. How-To Videos Screencapture walk-throughs to help guide you through Addgene's...
Showing: 161 - 200 of 402 results