We narrowed to 1,082 results for: TOR

...Resources Viral Vector Production Learn about how we produce high-quality viral vector preps at Addgene...Request service with all-in-one pricing for viral vector preps, which includes MTA facilitation, DNA amplification... Viral Vector Packaging Service Packaged on Request AAV Packaged...Additional Resources Adeno-associated virus (AAV) vectors are safe and useful gene delivery tools that effectively...protection levels. We offer 1,092 in-stock viral vector preps, delivering high quality and ready availability...availability at affordable prices. While our repository serves the needs of many researchers, scientists sometimes... the steps to produce, purify, and titer viral vectors. Overview Our new Packaged on Request service is...

...Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic acid and Thyroid hormone receptor (NCoR/...Cell Line Repositories (Link opens in a new window) Coriell Institute - cell line repository, including...in a new window) MMRRC Repository - NIH-supported mouse and cell line repository (Link opens in a new window...largest biorepositories (Link opens in a new window) ATCC - the world's largest cell line repository Tissue... working with the RSRT along with individual laboratories to assemble a Rett Syndrome plasmid resource...The MECP2 protein is a global transcriptional regulator of thousands of genes and studies have suggested...location and type of MECP2 mutation can be a strong predictor of symptom severity. Broadly, early gene disruptions...

...targeting vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species Mammalian Bacteria Drosophila Plant ...Cas plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA, based on ...tagging system were tested by tagging transcription factors with FLAG in human cell lines. To repeat the tagging...the isolation of native protein complexes. This vector serves as a backbone to clone the left and right...Alternatively, cDNAs can be cloned directly into this vector and targeted to the AAVS1 genomic safe harbor locus...gRNA_AAVS1-T2 (Addgene #41818) or using an all in one vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_...

....org Adenoviral Vector Guide All Viral Plasmids Viral Vectors 101 eBook Viral Vectors 101 YouTube Videos...bacterial strains for adenoviral vector production in Addgene's repository. Viral... can be used for adenoviral vector production. Read our adenoviral vector guide for more information about...Adenoviral Vector Guide Shuttle Plasmids Browse commonly used shuttle plasmids for adenoviral vector production...adenoviral vector production. ID Plasmid / Kit Description PI 16400 pAdEasy-1 Adenoviral vector for use ...Adenoviral Plasmids Adenoviral Strains Adenoviral vectors are widely used for gene delivery due to their ...production. In order to produce recombinant adenoviral vectors, researchers generally need to use two plasmids...

...more details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled...dCas9) can be fused to a transactivator and used as a transcriptional activator . ID Name Insert Antibiotic...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently ... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus... made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive...receive a ready-to-use viral preparation from the inventory below. Lentiviruses are distributed as purified...dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP Zhang 61425 lenti dCAS-VP64_Blast ...

... gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) ... dCas9 activators sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...webtool that uses deep learning models for base editor gRNA design and efficiency prediction. Developed...webtool that uses deep learning models for prime editor pegRNA design and efficiency prediction. Developed... Lab and maintained by the Frederick National Laboratory at the National Cancer Institute. Cas-OFFinder...information from Cas-OFFinder and Microhomology predictor. Developed by the Jin-Soo Kim Lab . CRISPR-ERA...

...upper and lower motor neurons in the brain and spinal cord. Over time, a loss of motor neurons leads to... with improved glutamate indicators . (Aggarwal et al. bioRxiv. 2025) Monitor dopaminergic activity in...using three transcription factors in a single polycistronic lentiviral vector . (Addis et al. PLoS One....CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other Resources...95%) of ALS are sporadic, having no prior family history. A small percentage (5–10%) are familial ALS cases...portion of cases can be attributed to known genetic factors such as defects in LRRK2, SNCA, PARK7, or PINK1... gene variation and lifestyle or environmental factors. For example, variations of Apolipoprotein E (APOE...

...Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout...ligand-binding domain such as the human estrogen receptor (ERT2) so that the enzyme is only activated in...you can do with it, read Addgene's blog on FLEx Vectors . Flp-IN: A single FRT site within the genome of...to minimize leakiness in experiments using AAV vectors. Highlighted Plasmids Browse the tables below to...Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources Explore...and filter the results by expression system and vector type. Find in-stock viral preps expressing Cre ...Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources Search...

...Molecular Tools Viral Vector Packaging Service: Molecular Tools Tetracycline Transactivators Affinity Purification...encoding tet-off transactivators and tools for affinity purification (TRAP). Viral... Viral Vector Packaging Service...Labeling Overexpression Tracers Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible...-inducible tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible...CAG-driven, constitutive Expression of the tet-off transactivator (tTA). 2 Viviana Gradinaru 99120 pAAV-ihSyn1... promoter (ihSyn) Expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified...

...and synthetic regulatory elements, including promoters, terminators, repressors, activators, and more. Also... Lab Repressors , Terminators , Light Signaling , Sigma Factors , Resource Allocator and Orthogonal Switches...cerevisiae Advanced Gateway Destination Vectors TAL Effectors Zinc Finger Consortium Community Resources...including shuttle, integration, reporter, and tagging vectors. Metabolism Browse plasmids related to metabolic...Plasmids and Phagemids Balazsi Lab Negative Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins...Plasmid Kit Gray Lab Maize and Rice Transcription Factors Hasty Lab Biopixels Jaramillo Lab Riboregulation...Biological Parts (Link opens in a new window) — Repository of community-created BioBricks JBEI (Link opens...

...Expression Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL...Reagents from the Keith Joung laboratory for engineering TAL effectors, including designed TALENs, and...TALEN Kits TALengineering Reagents Joung Lab TAL Effector Engineering Reagents You may also like... Keith... developed three platforms for engineering TAL effector repeat arrays of any desired length: the REAL ...2012). All three of these platforms assemble TAL effector repeat arrays on a single architectural framework...all three methods are identical in sequence. TAL effector repeat arrays produced on this framework has yielded..., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., Nat Methods 2013...

.... pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS),...encode zebrafish receptors or monoclonal antibodies to zebrafish cell surface receptors and secreted proteins...found below: UFlip CRISPR targeted integration vectors for cell-type specific conditional gene inactivation...inactivation - Jeffrey Essner Lab GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner...mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish...zebrafish Whole organism lineage tracing by combinatorial and cumulative genome editing - Jay Shendure...with four promoters, six fluorophores, and empty vectors designed to streamline the production of transgenic...

...antibodies can be stored at 4 °C for short-term storage. For long-term storage they should be stored at -20 °C... Antibody Handling Storage Check the manufacturer’s instructions for exact storage requirements. As a ...tips for storing your antibody: Antibodies conjugated to fluorophores should never be stored below 4 °... antibodies should not be stored in sodium azide, as it acts as an inhibitor to HRP. In those cases, thimerosal...define key terms, summarize antibody production and storage techniques, and explain common applications. Science...expensive than polyclonal antibodies. Environmental factors, binding partners, location in the cell, and sample... a primary antibody, which becomes the limiting factor for the number of proteins you can detect in a ...

...a bacterial stab (of a laboratory E. coli strain). Addgene continues to monitor its library and distributions...Broad-Range Rainbow Vectors - Barbier Lab Cloning and Expression Bacteria and yeast expression vectors : FX cloning...Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range of microbial...plasmid tools for the community that aren't in the repository? Help us widen our resources for the community...protein expression in E. coli , B. subtilis and P. pastoris - Gomis-Rüth Lab pOSIP plasmid kit : Integration...Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly of up to three transcription units in ...lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion proteins, etc -...

...reduced immune effector functions Expression of IgG Monoclonals with Engineered Immune Effector Functions....antibodies. Description Article PI Minimal expression vectors for transient mammalian protein expression...glycoprotein production with an open-source expression vector. bioRxiv 2026.03.18.712111. Institute for Protein...light-chain DNAs into a single mammalian expression vector A tool kit for rapid cloning and expression of ...1827:313-334. Daniel Christ pComb3 phage display vector system for producing human antibodies Synthetic...Carlos Barbas Variant of the pComb3 phage display vector system for isolation of scFv, diabody and Fab fragment...Methods. 2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression, dimerisation and detection...

...Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1 - Mammalian Expression...Mammalian Gateway Destination Vector pENTR4-HaloTag (w876-1) - Gateway Entry Vector pCS2+/C-Halo , pCS2+/N-Halo...Gateway Entry Vector pCS2+/C-SNAPf , pCS2+/N-SNAPf - Xenopus Expression pSNAP-tag (T7) Vector - Bacterial...pCLIP-tag (m) Vector - Mammalian Expression pENTR4-CLIPf (w877-2) - Gateway Entry Vector pET51b-His-TEV-CLIP-tag... or search our complete collection to filter by vector type, popularity, and more. Blue/UV Protein Excitation...93 5.7 10 min Monomer pCS2+mNeonGreen-C Cloning Vector - Mammalian Expression Return to top Yellow Protein...Xenopus/Worm/Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-pBAD...

...Two classes of base editors — cytosine base editors (CBEs) and adenine base editors (ABEs) — can be used...base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor. Both... without double stranded breaks. Cytosine base editors are created by fusing Cas9 nickase or catalytically...dCas9) to a cytidine deaminase like APOBEC. Base editors are targeted to a specific locus by a gRNA, and...on the opposite strand). Likewise, adenine base editors have been engineered to convert adenosine to inosine...Both types of base editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further...increase product purity such as the DNA glycosylase inhibitor (UGI) or the bacteriophage Mu- derived Gam protein...

...engineered into viral vectors for safe use in the lab. One safety feature of lentiviral vectors is that the components...LTRs Packaging plasmid — contains packaging and regulatory genes; one plasmid in second-generation, two ...plasmids available at Addgene. Read our Lentiviral Vector Guide for more information about lentiviral components... viral production, and viral safety. Lentiviral Vector Guide Popular Lentiviral Transfer Plasmids Looking...promoter. CMV-EGFP reporter cassette is included to monitor expression. Luk Parijs 11619 pLB 3rd Modification...promoter. CMV-EGFP reporter cassette is included to monitor expression. Stephan Kissler 1730 pLionII 3rd FIV-derived...FUW-M2rtTA 3rd Contains reverse tetracycline transactivator. Rudolf Jaenisch 131626 pHAGE-mt-mKeima 3rd...

...support educators interested in integrating MDH-related research into their teaching laboratories. They ...access to mentors, virtual and local professional development, membership to a local hub of educators teaching... Depositor Collections MDH CUREs Community Malate Dehydrogenase...They encourage instructors teaching different types of courses, at any type of institution, and at different...teaching MDH CUREs, and opportunities to mentor future MCC members. Find a regional hub (Link opens in a ...a new window) and contact the hub director for more information. Why MDH? MDH is stable, inexpensive to...cloned into an IPTG-inducible His-tag expression vector. All His-tags are cloned at the C-terminus of ...

...Neuroligin (Link opens in a new window) receptor families, key mediators of synaptic adhesion and function....recombinant antibodies targeting axonal guidance receptors and ligands (Glypicans and Integrins), components...axon guidance signaling proteins and growth cone receptors. Developed using yeast display technology, these...high-confidence targeting of proteins that have historically been difficult to identify and interrogate with...conventional reagents. Figure 1: Axon guidance cues and receptors regulate the development of functional neural ...development. Figure 2: Key adhesion proteins and receptor–ligand pairs bridge the presynaptic and postsynaptic...Integrins are a family of mechanotransduction receptors, comprised of alpha-beta subunit heterodimers....

... top Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing are...tevopreq1-GG-acceptor Mammalian hU6 epegRNA BsaI No mRFP1 David Liu 174039 pU6-tmpknot-GG-acceptor Mammalian...Find prime editor plasmids for (search and replace) genome editing. CRISPR...enzyme is named PE1 . PE1 and most other prime editors use the Moloney murine leukemia virus (M-MLV) reverse...can be used in PE2–PE5 systems PE6 — small prime editors with optimized RT and/or Cas9 domains PE7 — addition...addition of RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA stability epegRNA —... table below. Use the search bar to find a gRNA vector based on expression system, promoter, the type ...

... Viral Vector Packaging Service AAV Chemogenetics Viral Vector Packaging Service: Chemogenetics...information about this field. Receptor Activating hM3D(Gq) DREADD rM3D(Gs) DREADD Inhibitory HCAD(Gi) DREADD hM4D...Promoter Synapsin CaMKIIa CD68 Dlx GFAP nEF CAG E2 regulatory element Tag Fusion tags mCherry HA Non-fusion...AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype...pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid receptor (KOR) - Inhibition IRES mCitrine Cre-dependent...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Browse all Chemogenetics AAV Don’t See ... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...

... Depositor Collections Allen Institute for Brain Science ...Enhancer AAV plasmids are plasmids which use genomic regulatory sequences — termed enhancers — to drive cell ... AAV delivery. The collection includes the top vectors with the highest specificity and brightness, assessed...pAAV-AiE2563m-minBG-SYFP2-WPRE3-BGHpA AiP1969 AiE2563m SYFP2 Entorhinal area - L2 pyramidal neurons Hippocampal formation...pAAV-AiE2403m-minBG-SYFP2-WPRE3-BGHpA AiP1772 AiE2403m SYFP2 Entorhinal area - L2 pyramidal neurons Hippocampal formation...pAAV-AiE2664m-minBG-SYFP2-WPRE3-BGHpA AiP2107 AiE2664m SYFP2 Entorhinal area - L3 pyramidal neurons Hippocampal formation...pAAV-AiE2664m-minBG-iCre(R297T)-BGHpA AiP20169 AiE2664m Cre Entorhinal area - L3 pyramidal neurons Hippocampal formation...

...interest. Browse Cancer Research Plasmids by Pathway mTOR mTOR is a protein kinase that anchors a signaling network...Cancer Research Broad Target Accelerator Plasmids: Collection of vectors from the Boehm , Hahn , Meyerson...mutated gene in human cancer, is a transcription factor and tumor suppressor. Wnt Coming soon! Kits and...mutant alleles described in the initial Target Accelerator publications, focused on somatic variants found...pan-cancer” analyses. Cancer Pathways ORFs Kit : Set of vectors from the Sabatini & Wood Labs containing cDNAs ... Lab RAS Pathway Clone Collection 2.0 : Set of vectors from the Esposito Lab for use with the Gateway®...permit construction of RAS pathway gene expression vectors for in vitro and in vivo research. TP53 Mutagenesis...

...basic-hFX-promotor(1kb) FXN hFXN Friedreich ataxia Michael Ristow 14979 pGL3-basic-hFX-promotor(1,3kb) FXN...basic-hFX-promotor(2,5kb) FXN hFXN Friedreich ataxia Michael Ristow 14981 pGL3-basic-hFX-promotor(2kb, distal...TARDBP His, GST, Factor Xa cspA ALS Aaron Gitler 27452 pCold TDP43 G294A TARDBP His, Factor Xa cspA ALS Aaron...M337V TARDBP His, Factor Xa cspA ALS Aaron Gitler 27454 pCold TDP43 Q331K TARDBP His, Factor Xa cspA ALS Aaron... DCTN1 CMV ALS, motor neuron disease Trina Schroer 51221 CMV-CC2 DCTN1 CMV ALS, motor neuron disease Trina... ALS, motor neuron disease Caren Norden 105971 pME mKate2-dynactin1-811 DCTN1 mKate2 ALS, motor neuron...237005 VCP-HaloTag Vector VCP Halo CMV ALS Promega Corporation 237006 HaloTag-VCP Vector VCP Halo CMV ALS...

... Viral Vector Packaging Service AAV Serotype Testing AAV Viral Vector Packaging Service: ...available from Addgene's viral service. Control EGFP vectors in various serotypes for serotype testing: AAV1...addition to the standard 100 µL sample size, the vectors listed below are also available as 20 µL. The ...experiments. For more information, see our full AAV inventory or our AAV production page . Note: For a list ...100 µL sized sample, please see our Control AAV Inventory . The catalog ID for the 20 µL sized testing sample...does not have the suffix (for example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...

...cellular receptors. The cellular receptor for SARS-CoV-2 and SARS-CoV is the angiotensin receptor, Angiotensin...Additional Addgene resources ACE2 plasmids - Host cell receptor mediating the entry of SARS-CoV and SARS-CoV-2...Cytokines - SARS-CoV-2 infection induces a cytokine storm response in the body. For a full list of cytokines...) Ectodomain and RBD mutants. SARS-CoV-2 Spike Receptor Binding Domain Deep Mutation Scanning Library ...mutations for comprehensive mapping. SARS-CoV-2 Spike Receptor Binding Domain Site-Saturation Mutagenesis Libraries...Cathepsin L functionally cleaves the severe acute respiratory syndrome coronavirus class I fusion protein upstream...TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein . Journal ...

...viral vectory packaging service. Browse constructs currently available in viral format.... Viral Vector Packaging...Browse In-Stock AAV Adeno-associated virus (AAV) vectors are safe and useful gene delivery tools that effectively... in small volumes, and ready to ship. All viral vector preps are purified by density centrifugation. Addgene...service. You can choose a transfer plasmid from our repository and request packaging in one of eight serotypes...or AAVrg). For more information, visit our Viral Vector Packaging Service overview. Function Chemogenetics...Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation, CRISPR...

...Surface Display cDNA Depositor Collections A modular set of plasmids from a depositor organized by a common...Collections Addgene Plasmid Collections Addgene's repository contains 172,416 plasmids . We've gathered our...our curated plasmid resources, kits, and depositor collections on one page to help you find what you're...looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources group...common use. Click here to view all collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein... Protein Expression Synthetic Biology Viral Vectors Communities Find a scientific community that has established...Variation on Function (IGVF) Consortium Krüppel-like factors (KLF) Plasmid Resource RESOLUTE Plasmid Collection...

... Viral Vector Packaging Service AAV Controls Viral Vector Packaging Service: Control...quality AAV preps from select plasmids in the repository. Browse our control AAV collection, including...Karl Deisseroth 135630 pAAV-S5E2-dTom-nlsdTom E2 regulatory dTomato Constitutive 1, 9, PHP.eB Jordane Dimidschstein...Dimidschstein 135631 pAAV-S5E2-GFP-fGFP E2 regulatory EGFP Constitutive 1, 9 Jordane Dimidschstein 135641...135641 pAAV-S5E6-dTom-nlsdTom E2 regulatory dTomato Constitutive 1 Jordane Dimidschstein 137135 pAAV-Ef1a-oScarlet...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Our ... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...

... Viral Vector Packaging Service AAV Monosynaptic Neuronal Tracing Viral Vector Packaging Service... Addgene does not distribute rabies virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description...specifically into cells that express the TVA viral receptor. Thus, by pseudotyping the rabies virus with EnvA...specifically targets the starter cell via the EnvA receptor. RABV is able to replicate in the starter cell...free to infect neighboring cells via G-protein receptors, which are naturally expressed at presynaptic ... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

...luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing tags such...luciferase reporter vectors for sensitive gene expression analysis and protein fusion vectors for detecting ... Depositor Collections Promega Plasmid Collection Promega...sensitivity. These assays are ideal for real-time monitoring of signaling pathways, investigating RNA regulation... without disturbing the virus of interest and monitoring endogenous protein dynamics in highly scalable...with the NanoBRET 618 fluorophore, as the energy acceptor. Live-cell reagents allow you to detect PPI induction...

...fluorophores, excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting... Depositor Collections INTRSECT Plasmid Collection INTRSECT...INTRSECT (intronic recombinase sites enabling combinatorial targeting) is a synthetic molecular targeting...Flp AND NOT Cre Yes Dual recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations...Flp AND NOT Cre Yes Dual recombinase-dependent: Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations...Deisseroth K. 2014. Targeting cells with single vectors using multiple-feature Boolean logic. Nat Methods...Krook-Magnuson E. 2019. A novel population of long-range inhibitory neurons. Preprint (Link opens in a new window...

... Viral Vector Packaging Service AAV Optogenetics Viral Vector Packaging Service: Optogenetics...quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV collection, including...plasmids available in Addgene's collection. Opsin Excitatory Wild-type ChR2 ChR2/H134R soCoChR ChR2/other ... Chrimson, ChRmine, ChroME, ChETA, C1V1 (t/t) Inhibitory Arch and variants eNpHR and variants InSynC (...Bidirectional BiPOLES Promoter CAG CaMKII CBA Dlx E2 regulatory element EF1a/nEF GFAP Synapsin TRE Fluorophore...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Our ... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...

...Resources SGC: www.thesgc.org SGC Toronto: www.thesgc.org/scientists/groups/toronto Protein Database (PDB): www.pdb.org...without restriction. Many SGC plasmids from the Toronto, Oxford and Stockholm sites have been deposited...the SGC Plasmid Collection By All Plasmids SGC Toronto SGC Oxford SGC Stockholm Bacterial Expression Plasmids...Thrombin cleavage, Bac-to-Bac Baculovirus Expresion vector 26117 pNIC-CH EF199843 Hexahistidine tag, AmpR ...cleavage, AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine... System, AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with C-terminal hexahistidine...

...shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred method for long-term ...Plasmid Vector Type PI Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol...long-term gene silencing. Once the viral vector is taken up by a host, the viral genome is transcribed, producing.... shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a lentiviral...Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design in pSico with...

...Empty gRNA Vectors Select an empty vector for your experiment based on a variety of factors. Browse Plasmids...for you to download. CRISPR Resources : Browse depositor protocols, find software for gRNA design and deep...discover links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial...information into a specific DNA site. Base Edit Base editors precisely edit DNA without promoting DSB formation...Transcriptional Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9...

...permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single round of viral entry...NL4-3 mCherry Luciferase Lentiviral dual reporter vector expressing mCherry and firefly luciferase to simultaneously...105621 Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly luciferase with neomycin resistance...pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro Lentiviral vector expressing destabilized EGFP. Neville Sanjana 164432...pHAGE-CMV-Luc2-IRES-ZsGreen-W Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen. Alejandro...David Nemazee 161030 Zhou-pLOV-GFP-Luc Lentiviral vector expressing GFP and luciferase for use as a reporter...coronaviruses (DOCX, 184 KB) Addgene Blog Posts Viral Vectors 101: Pseudotyping Antibody Neutralization Response...

...signed by an authorized signatory at the recipient institution. An authorized signatory is someone who can ...also adding additional filters such as expression, vector type, species, and more. Plasmids Browse the table...Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive end-joining ligation...Genome Editing Jim Haseloff 79 parts plasmids and vectors for genome engineering in the plant model Marchantia...Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome...entity is qualified to receive materials from our repository. All purchases are subject to Addgene's Terms...

...mEGFP AICSDP-60 mEGFP Transcription factor SOX-2 Transcription Factor 124607 ACTN2-mEGFP AICSDP-63 mEGFP...repeat-binding factor 2 (TRF2) Telomeres 168799 CTCF-mEGFP AICSDP-144 mEGFP CCCTC-binding factor (CTCF) CTCF-binding... Depositor Collections Allen Institute for Cell Science Plasmid...UBTF-HaloTag AICSDP-80 HaloTag Nucleolar transcription factor UBF Nucleolus (fibrillar center) 133964 SON-mEGFP...TFAM-mEGFP AICSDP-72 mEGFP Mitochondrial transcription factor A (TFAM) Mitochondrial nucleoids 164499 EZH2-mEGFP...