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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 232 results for: SPL;

Showing: 201 - 232 of 232 results

  3. March for Science

    Type
    Blog Post
    ... that thoughts on some non-political issues are split along party lines (18). Feeling like an apple will...

  5. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...digest products of the unmodified plasmid will be split into two smaller bands (Figure B, digest A). In ...

  9. Antibodies 101: Validation

    Type
    Blog Post
    ...alternative translation start sites or alternative splicing that could lead to some form of the target protein...

  17. Weighing Reagents Protocol

    Type
    Protocol
    ...provide more accuracy as these types of scales display more decimal places. Pro-Tip If you’re weighing...

  18. Water Bath Protocol

    Type
    Protocol
    ...bath, but will likely involve a dial or a digital display you can set to the correct temperature. Set up ...

  20. General Transfection

    Type
    Protocol
    ...obtaining high levels of virus. HEK293T cells should be split 3 times a week: Monday: Plate 1x10 6 cells in a ...

  21. Gibson Assembly Protocol

    Type
    Protocol
    ...correctly assembled plasmids by designing primers to split an antibiotic resistance gene to effectively create...

  22. Lentivirus Production

    Type
    Protocol
    ...obtaining high viral titer. 293T cells should be split 3 times a week: Monday: Plate 1×10 6 cells in a ...

  24. CRISPR Library Amplification

    Type
    Protocol
    ... is better than pulling motion Take care not to split or gouge agar during the scraping process. Add each...

  25. 27 Hot Plasmids from 2016

    Type
    Blog Post
    .... Each gene was chosen to represent the primary splice variant in 38 types of human cancer. Many of the...

  26. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...AAV8, AAV9 Table 1: Summary of tissue tropism displayed by different AAV serotypes. Pseudotypes Pseudotyping...derived from eight different serotypes. AAV-DJ displays a higher transduction efficiency in vitro than...specific cell and tissue types, like AAV-DJ8, which displays enhanced brain uptake. Systemic capsids Another...employed to engineer an rAAV serotype variant able to display neuronal retrograde transport, AAVrg. Retrograde...limitation for CRISPR delivery for more information. Split AAVs to increase packaging capacity Another strategy...derived plasmids and base/prime editors, is to create split AAVs. Here, a longer transgene is broken down between...different mechanisms. One of these methods uses splice site donor and acceptor sequences to reconstitute...

  27. Sequencing Primers

    Type
    Guide
    ...promoter/origin, forward primer SV40-spliceR CACAAAGATCCGGACCAAAG SV40 splice sequence, reverse primer T3 GCAATTAACCCTCACTAAAGG...vector, forward primer SFFV-F ATTGATTGACTGCCCACCTC Spleen focus forming virus 5' LTR, forward primer SP6 ...

  28. Lentiviral Vector Guide

    Type
    Guide
    ... Rev Response Element (RRE) within unspliced and partially spliced transcripts to facilitate nuclear export... a few key ways. First, the packaging system is split into two plasmids. Although safer, this system can...

  29. CRISPR Guide

    Type
    Guide
    ... reverse transcription. The edited DNA strand displaces the unedited 5’ flap, and the resulting heteroduplex...including editors based on a second ADAR variant that displays improved specificity, and editors carrying an ... in vivo RNA imaging or to control alternative splicing. Figure 13: Overview of RNA targeting (A) and ...locus-specific targeting of long RNAs with CRISPR-Display. Nature Methods , 12 (7), 664–670. PMID: 26030444...

  30. Plan Your Experiment

    Type
    Guide
    ...should be made HDR requires a repair template and displays reduced efficiency compared to NHEJ knockout. ...region is removed from the mRNA due to alternative splicing. Exons near the N-terminus are targeted since ...

  31. Optogenetics Guide

    Type
    Guide
    ...photocurrent amplitude 490 ChR/T159C T159C mutation; displays increased photocurrents 470 SFO/SSFO Step function...

  32. Adenovirus Guide

    Type
    Guide
    ...26–45 kb in length and contain multiple heavily-spliced transcripts flanked by two inverted terminal repeats...
Showing: 201 - 232 of 232 results