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We narrowed to 273 results for: aav

Showing: 201 - 250 of 273 results

  3. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...pX601 61591 Mammalian/AAV BsaI yes, cut S. aureus Zhang pX602 61593 Mammalian/AAV BsaI yes, cut S. aureus...pyogenes Joung AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR 68347 Mammalian/AAV none S. pyogenes...pyogenes mCherry Kuhn AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR 60229 Mammalian/AAV SapI none S. pyogenes...pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR 60231 Mammalian/AAV SapI none...pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR 60226 Mammalian/AAV SapI none S...particular expression system , eg. Mammalian, Lentiviral, AAV, Bacteria, Drosophila, Yeast, Plant, Xenopus, Worm... none S. pyogenes Gersbach PX552 60958 Mammalian/AAV SapI none S. pyogenes Zhang pgRNA-humanized 44248...

  4. Hot Plasmids - February 2022

    Type
    Blog Post
    ...off-target effects, yet small enough to be packaged in AAV. A dCasMini-mediated adenine base editor allows for...

  6. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...been deposited to Addgene, including lentiviral and AAV expression vectors for certain variants. Klapoetke... infeasible. Finally, an adeno-associated viral (AAV) system enables greater spatio-temporal control over... either the Thy1 or CAG promoter, while Brainbow AAV is under control of the EF1a promoter. Livet et...

  7. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... 74307 pAAV-CMV-DIO-Magneto2.0-sNRPpA AAV 74306 pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA AAV 74302 ...plasmids, which already includes dSaCas9-VPR in an AAV transfer vector for in-vivo expression and plasmids...Magneto2.0 in the mammalian brain after delivering it via AAV injection, finding that it could increase the activity...lines by inserting OsTIR1 into the "safe harbor" AAVS1 locus (a tet-inducible OsTIR1 plasmid is also available...

  8. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...protein production AAV-GfaABC1D-MCS--4x6T-WPRE - For astrocyte-selective expression in AAV Dual promoter Separate...Advantages Representative Empty Backbones AAV High transduction efficiency, but do NOT integrate...expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page Lentiviral Can transduce both dividing...

  10. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...it easier to package for therapeutic delivery by AAV, which has strict capacity limitations. New efforts...Science, 362(6416): 839-842 (2018). 10.1126/science.aav4294 Ozcan, Ahsen, Krajeski, Rohan, Ioannidi, Eleonara...

  12. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...mGreenLantern - Mammalian Expression pAAV-CAG-mGreenLantern - Mammalian AAV Expression pBAD-mGreenLantern -...Crimson 611 646 29 4.5 24 min Tetramer pAAV-CAG-E2-Crimson - Mammalian AAV Expression pEB2-E2-Crimson - Bacterial...pcDNA-miRFP670nano3 - Mammalian Expression pAAV-hSYN-miRFP670nano3-WPRE-hGHpA - Mammalian AAV Expression pBAD/His-miRFP670nano3...pmiRFP680-N1 - Mammalian Expression pAAV-CAG-miRFP680 - Mammalian AAV Expression pDRF1 ymiRFP680 - Yeast...Expression miRFP713 690 713 7 3.5 Monomer pAAV-CAG-miRFP713 - Mammalian AAV Expression pmiRFP713-N1 - Bacterial...pmiRFP720-N1 - Mammalian Expression pAAV-CAG-miRFP720 - Mammalian AAV Expression Return to top Long Stokes...

  15. Adenovirus Guide

    Type
    Guide
    ...for replication. For more information about AAVs, read our AAV guide . Recombinant Adenoviral Vectors Wild-type...double-stranded DNA genome. Adeno-associated viruses (AAV), however, are small single-stranded DNA viruses ...viruses that belong to the Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations...how they got their name, but they are not related. AAVs require the presence of adenoviral genes E1, E4,...Vectors Although recombinant adeno-associated viral (rAAV) vectors are often the preferred choice among researchers...expression cassettes are often too big to be packaged in rAAV vectors. This is the case of some commonly used ...first-generation rAdV vectors) surpasses that of rAAV vectors (~4.7 kb) and is further increased in second...

  19. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...targeting expression involve tissue specific promoters, AAV serotypes, and the ever-growing variety of recombinase...applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282.long Redfern CH, ...

  21. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...capabilities but are small enough to be packaged in AAV particles. Figure 1: Overview of RNA targeting with...

  22. Depositor Collections

    Type
    Collection
    ...Protein Collection Allen Institute for Brain Science AAV Enhancer Collection Allen Institute for Cell Science...

  24. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...Lee et al., 2024. The authors have deposited AAV plasmids for Cre-dependent and -independent versions...for the first time (Figure 3). The team also used AAV9 to deliver ASAP5 to the cortex and imaged voltage...

  27. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...Thy1-YFP-H mice).  However, we recently developed AAV (adeno-associated virus)-based fluorescent labeling...cord and cranial nerve afferents labelled with a AAV8 expressing eGFP under the human ubiquitin C promoter...

  28. Plasmid Collections

    Type
    Collection
    ...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...

  30. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...glycoprotein along with the TVA receptor in neurons using AAV and then delivering rabies dG pseudotyped with EnvA...

  32. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Titration Assay Lentivirus Fluorescence Titration Assay AAV Titration by qPCR Addgene Viral Service Lentivirus...

  33. Mammalian RNAi Tools

    Type
    Collection
    ... a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive expression...

  37. Science Guides

    Type
    Guide
    ...research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus Read More...

  38. Adenovirus Plasmids

    Type
    Collection
    ...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are...

  40. Distribution to Industry

    Type
    Collection
    ...Carrier Proteins (SLCs) from the RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from ...

  41. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... RNA-H1-nick sgRNA-mCherry Mammalian, AAV hU6 + H1 pegRNA + nicking sgRNA No mCherry Hyongbum...

  42. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    .... We also offer ready-to-use AAV preparations of select plasmids and AAV Packaged on Request for many ...Luciferase Plasmids Optogenetics Plasmids Viral Service: AAV Biosensors Fluorescent biosensors are proteins designed...Commun. 2018 Oct 25;9(1):4440. Eric Schreiter Calcium AAV expression of photoconvertible fluorescent protein-based...2024.12.16.628673. Olivia Masseck Calcium Astrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent...voltage imaging. Science. 2019 Aug 1. pii: science.aav6416. Eric Schreiter Voltage Green fluorescent voltage...

  45. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs also have distinct PAM sites that...also makes it suitable for multiplexing and use in AAV. Scientists have also developed CRISPR editing technologies...

  49. Deep Dive: qPCR

    Type
    Blog Post
    ...resources on the Addgene blog Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...

  50. CRISPR Guide

    Type
    Guide
    ...efficiently packaged into adeno-associated viruses (AAVs) . Other natural Cas orthologs include Hsp1Cas9 ...Mammalian CRISPR libraries have also been created in AAV backbones for in vivo experiments and in a retroviral...capabilities but are small enough to be packaged in AAV particles. Cas13 fusions have also been used in in...
Showing: 201 - 250 of 273 results