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...Addgene Parkinson's Research from The Michael J Fox Foundation Search Use Addgene's Search to find plasmids...ONE-GO Biosensors Kit Fluorescent Proteins Mikel Garcia-Marcos 10 plasmids for detecting GPCR activity in a... Distribution to Industry Featured Collections Search Plasmids Plasmids Kits Pooled Libraries Antibodies...Protein Labeling from New England Biolabs Cancer Research from Sinai Health System Plasmids from the Structural...vectors for genome engineering in the plant model Marchantia polymorpha . Pooled Libraries Pooled Library ... Library Screening Messer and MJFF Created for research in Parkinson's Disease; For target validation ...open, efficient and affordable access to plasmid research tools. Pricing will be indicated on the product...

...highest-possible quality of research output, RESOLUTE expects to accelerate the pace of research in the field of ...global benefit of basic academic research as well as applied research in small biotech and pharmaceutical...mission is to develop data and reagents to boost the research focused on Solute carrier proteins (SLCs). Browse...mission is to develop data and reagents to boost the research focused on SLCs. Through the coupling of an inclusive...support from the European Union's Horizon 2020 research and innovation programme and EFPIA. Figure 1: ...Overexpression Library 213694 Superti-Furga Inducible barcoded overexpression library of human SLC proteins. ...S., Barbosa, B. M. G., Onstein, S., Klimek, C., Garcia, J., Serrano, I., Reil, D., Santacruz, D., Piotrowski...

...window) encourages researchers to assemble and share tools for Parkinson's disease research. This collection...Neurodegeneration Research Collection , which includes other areas of this research topic. Michael J. ...Shared Research Tools Labs affiliated with MJFF have also generated a number of Shared Research Tools ... Disease Plasmid Resource MJFF Plasmids Shared Research Tools (Link opens in a new window) The Michael...SNCA , and other proteins of interest. Browse or search the table below. ID Plasmid Gene/Insert Mutations...editing tools deposited with Addgene. Browse or search the table below. ID Plasmid Description Gene/Insert...

...Broswe plasmids and other research tools for use in Rett Syndrome research....The (Link opens in a new window) Rett Syndrome Research Trust (RSRT) was launched in 2008 to drive the...Rett Syndrome plasmid resource for the scientific research community. Background Rett Syndrome Rett syndrome...account for almost 46% of all Rett syndrome cases. Research Milestones 1992 - MECP2 is discovered by Adrian...syndrome and restore functional MECP2 in cells. Search (Link opens in a new window) JAX Mice for mouse...plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR Tools CRISPR technology is a promising... that express or target the MECP2 gene. Use the search bar or sort buttons to find plasmids based on: ...

...promotes and advocates for zebrafish research in the international research community. The Latin American Zebrafish...based gene editing make the zebrafish a stalwart research model. With more than 70% of human disease genes...model system for human disease and translational research, including the study of cancer, inflammation and...plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that contain ...plasmids that contain tools for use in zebrafish research. This includes plasmids for genome editing, empty...Periklis Pantazis Lab Multibow: digital spectral barcodes for cell tracing - Sean Megason Lab Zebrabow -...Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch Lab. A vertebrate model to study ALS-linked...

...mutant clones for your course-based undergraduate research experience (CURE) projects....Collection Blog post: Course-Based Undergraduate Research Experiences (CUREs) (Link opens in a new window...develops protein-centric Course-based Undergraduate Research Experiences (CUREs) centered on a single enzyme...national group of educational leaders providing research opportunities to their students and other members...educators interested in integrating MDH-related research into their teaching laboratories. They encourage...and mutants provides a shared experience for the research community and facilitates scientific collaborations...available to download from each plasmid page. Use the search bar or sort buttons to find plasmids based on: ...

... help you get started using CRISPR in your own research. CRISPR Basics In this section, we will introduce...same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic loci by cloning...Overview of Cas9n. Increasing fidelity of Cas9 Researchers have also enhanced specificity by engineering... overcome the CRISPR systems that bacteria and archaea use to protect against these foreign nucleic acids...Base Editing To overcome low HDR efficiency, researchers have developed base editors to specifically convert... 5: Overview of base editing. More recently, researchers have developed base editors that can catalyze...Base Edit CRISPR Prime Editing Prime editing is a “search and replace” gene editing method in which Moloney...

...proving to be just as useful (if not more so) for research applications, eclipsing past genome engineering...-strand break. CRISPR systems, found in 95% of archaeal and 48% of bacterial genomes, are highly diverse...proteins to the CRISPR-Cas complex. Recently, researchers have begun to use anti-CRISPRs as off-switches...activity due to rationally designed mutations. Researchers have expanded the CRISPR field beyond SpCas9,...allowing multiple Cas9s to be used together. Researchers have engineered mutant Cas enzymes with alternative...Despite the ethical controversies surrounding non-research applications, it’s clear that CRISPR is a truly...truly disruptive technology for basic research. The beauty of CRISPR is that it’s adaptable to almost any...

...AAV5 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 AAV production plasmid encoding for Archon1 fluorescent voltage...Li 115892-AAV8 pAAV-Syn-Archon1-KGC-GFP-ER2 AAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn...AAV8 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] AAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn...development of biomolecular tools for neuroscience research. Browse the BRAIN Initiative collection of plasmids...Initiative BRAIN Initiative Collection The Brain Research through Advancing Innovative Neurotechnologies...and circuits during behavior. Long desired by researchers, the revolutionary dynamic picture of the brain...emphasizes their rapid and broad dissemination to the research community. Browse plasmids , viral preps , and...

... for distribution to the research community. With these plasmids researchers will be able to generate ...skeletal type Sarcomeric thin filaments 114412 TTN-mEGFP AICSDP-45 mEGFP Titin Sarcomeres (M-line tag) ...mEGFP MLC-2a Sarcomeric thick filaments 114414 MYL2-mEGFP AICSDP-49 mEGFP MLC-2v Sarcomeric thick filaments...Cytoplasm 114405 ATP2A2-mEGFP AICSDP-41 mEGFP SERCA2 Sarcoplasmic reticulum/endoplasmic reticulum 114406 SMC1A-mEGFP...124607 ACTN2-mEGFP AICSDP-63 mEGFP Alpha-actinin-2 Sarcomeric z-disks 124608 NPM1-mTagRFP-T AICSDP-69 mTagRFP-T...

...Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent 5...Tommaso Patriarchi 187180 pAAV_hSyn1_nLightR Syn nLightR none Constitutive 9 Tommaso Patriarchi Oxytocin... 5 Edward Boyden 115892 pAAV-Syn-Archon1-KGC-GFP-ER2 Syn Archon1 EGFP Constitutive 8 Edward Boyden 115893...115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] Syn Archon1 EGFP Cre dependent 8 Edward Boyden Voltage Reporter...nLight Oxytocin (OT) Sensors GRAB_OT Voltage Sensors Archon Voltron JEDI-2P ASAP ATP Sensors iATPSnFR2 5-HT...

...Laboratory for Cancer Research . Frederick National Laboratory for Cancer Research Color is used for clarity...Laboratory for Cancer Research . Frederick National Laboratory for Cancer Research Return to top Ras Pathway... Cancer Research Ras Pathway Ras Pathway You May Also Like: Ras...Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral oncogene homolog GRB2 Growth factor receptor...exchange factor RAS KRAS MRAS NRAS Kirsten rat sarcoma viral oncogene homolog Muscle RAS oncogene homolog...gene expression vectors for in vitro and in vivo research. The RAS Initiative - at the National Cancer Institute...

...KB) Research Articles Many publishers allow free access to articles related to SARS-CoV-2 research: Cell...window) ASM COVID-19 Research Registry (Link opens in a new window) COVID-19 Open Research Dataset (CORD-19... tools and resources available for Coronavirus research. Plasmid...primed by the serine protease TMPRSS2. The global research community moved quickly to expand the knowledge...that are useful for COVID-19 related research. Additionally, we have linked to collections of...plasmid tools that may be helpful for COVID-19 research, including: CRISPR tools, like Cas12a and Cas13a...

...membrane channels and pores derived from algae and archaebacteria, that can be opened and closed in response ...microbial opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins, are pumps that...Guide . Interested in more news about optogenetics research? Find related posts on Addgene's blog. Microbial...Microbial Opsin Plasmids Browse, sort, or search the table below for the optogenetics plasmids for neuronal...neuronal excitation and inhibition. For example, search for "ChR mCherry", "Cre inhibition", or other terms....to top Optical Switch Plasmids Browse, sort, or search the table below for plasmids that use light to ...control a protein or cellular activity. For example, search "Cdc42", "transcription", or other terms. ID Plasmid...

...system of choice, promoter, or search by inducible system (e.g., search for "split", "rapamycin", “tamoxifen...been limited by low integration efficiency but research groups continue to optimize these tools. The best...recombinase (Cre, Flp, Dre, other recombinases). Use the search bars or sort buttons to find plasmids based on ...Vector Type Promoter PI Additional Addgene resources Search our full catalog for plasmids expressing Flp recombinase...Vector Type Promoter PI Additional Addgene resources Search our full catalog for plasmids expressing Dre recombinase... gene expression may be activated or repressed. Search by recombinase (Cre, Flp, Dre, etc.) or target ... sequence for your recombinase (see Table 1) to Search by Sequence to find other Cre-, Flp-, or Dre-dependent...

...synthetic biology collections) for use in C. elegan research....been developed and characterized which allow researchers to express desired constructs in precise cell...highlights plasmids that contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, ...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description...C. elegans strains. CRISPR plasmids from John Calarco's lab as described in Heritable genome editing in...Andrew Fire Lab. A set of vectors for C. elegans research, including lacZ and GFP fusion vectors. C. elegans...computer scientists dedicated to providing the research community with accurate, current, accessible information...

...In order to produce recombinant AAV vectors, researchers need to use three plasmids: A transfer plasmid...transfer plasmids in Addgene's repository. You can search or sort this table based on plasmid name, gene ... expressing Rep2 and Cap2 Melina Fan , Addgene Research Program 232922 pAAV2/5 AAV5 AAV packaging plasmid... expressing Rep2 and Cap5 Melina Fan , Addgene Research Program 240485 pAAV2/6 v1 AAV6 AAV packaging plasmid...expressing Rep2 and Cap6 Meghan Rego , Addgene Research Program 112863 pAAV2/7 AAV7 AAV packaging plasmid...expressing Rep2 and Cap11 Meghan Rego , Addgene Research Program 112866 pAAV2/rh10 AAVrh10 AAV packaging...separate helper plasmid during AAV production. You can search or sort this table based on plasmid name, capsid...

...possible function of a protein of interest. Browse or search the table below to find markers of general cellular...Mykytyn *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein...necessary for movement and cell division. Browse or search the table below to find markers of cytoskeletal...Harrison *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein...junctions, tight junctions, and desmosomes. Browse or search the table below to find markers for cellular junctions...Fanning *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein... autophagosomes, lysosomes, and more. Browse or search the table below to find markers for components ...

...Disease Research Find deposited plasmids for specific disease research areas. Cancer Research Plasmids...Collection Neuroendocrine Tumor Research Foundation Neurodegeneration Research Collection Rett Syndrome Plasmid...view all pooled libraries. Pooled Library Types Barcoding CRISPR Screening Surface Display cDNA Depositor...

...Lin Voltage Fluorescent voltage reporters Archon 1 and Archon 2 (Constitutive or Cre-dependent) A robotic...extracellular vs. organelle-localized). Browse, sort, or search the tables below highlighting a few popular categories...Genetically Encoded Calcium Indicators. Janelia Research Campus. Online resource. 2020 GENIE Project Calcium...: S2451-9456(16)30163-5. Teresa Alonso , Javier García-Sancho Calcium Genetically encoded calcium indicators...Nat Methods. 2023 Sep;20(9):1426-1436. Tommaso Patriarchi Norepinephrine GRAB_NE family of GPCR activation-based... Nat Methods. 2022 Feb;19(2):231-241. Tommaso Patriarchi Serotonin Red and green serotonin sensors GRAB...potentials in individual neurons and dendrites (ArcLight) Single action potentials and subthreshold electrical...