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Showing: 81 - 100 of 1006 results

  1. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Masato Kanemaki's lab has developed a simple and scalable CRISPR/Cas-based method to tag endogenous proteins...Geraldine Seydoux's lab has developed a systematic and scalable method to create marker-free mutations, insertions...co-integrated markers or footprints, and can be scaled up for systematic editing of multiple genes. Plasmids...

  2. Zhang Lab CRISPR Page

    Type
    Collection
    ... genes. It consists of three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating... Return to SpCas9 plasmids GeCKO Library Genome-scale CRISPR-Cas9 knockout screening in human cells. Shalem...PubMed . Return to GeCKO Library SAM Library Genome-scale transcriptional activation by an engineered CRISPR-Cas9...

  3. TALEN Expression Vectors

    Type
    Collection
    ...methods yield TAL effector repeat arrays with identical DNA and amino acid sequences (i.e. only the details...vectors also have a FLAG epitope tag and nuclear localization signal at their amino-terminus. The expression...

  4. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...epigenetic modifications, researchers have fused catalytically dead dCas9 to epigenetic modifiers. Design your...and Interfere technologies also indirectly modify local chromatin state. Want more information on the wide...

  5. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Catalytically inactive CRISPR plasmids for purification of target loci....DNA-binding molecule-mediated ChIP) system, catalytically inactive dCas9 is used to purify genomic DNA...

  6. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...CRISPR-associated transposases (CASTs) combine the large-scale capacity of transposases (Tns) with the precise ...several separate Cas enzymes that form a complex called Cascade ( C RISPR- as sociated c omplex for a ntiviral...

  7. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ... Resources: Empty Backbones FRET Subcellular localization Optogenetics Background In vivo imaging is a...interactions in deep-tissue organs and whole mammals. Genetically encoded fluorescent probes are suitable for stable...

  8. TALEN Engineering

    Type
    Collection
    ...and the DNAs produced by all three methods are identical in sequence. TAL effector repeat arrays produced...sites and then generates individual user-friendly graphical roadmaps for each target that guide the user through...

  9. Resolute Plasmid Collection

    Type
    Collection
    ...well as applied research in small biotech and pharmaceutical companies. The RESOLUTE project has received...interactions represent the framework that provides biological context. Assays monitoring impedance, fluorescence...

  10. Serotype Testing AAV

    Type
    Collection
    ...also available as 20 µL. The 20 µL option is an economical choice that can be used to validate the performance...performance of various serotypes before conducting larger scale experiments. For more information, see our full ...

  11. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator...see paper) Transfection none, need Cas9 plasmid Calarco 47943 pMB70 cU6 BsaI Transfection none, need Cas9...

  12. Retrovirus Plasmids

    Type
    Collection
    ...discuss retrovirus, they typically are referring to a subset of retroviruses called γ-retroviruses. γ-Retroviruses...

  13. Antibody Production

    Type
    Collection
    ...generated. The final antibody concentration is calculated from the standard curve. Using ImageJ software...order, ensuring each item ships safely and most economically. Actual shipping conditions will be shown in...

  14. Distribution to Industry

    Type
    Collection
    ...depositing institution(s). It governs the transfer of biological materials and intellectual property rights, and...Acknowledgement (RSA). This form can be completed electronically by the scientist who will be using the materials...

  15. Chemogenetics AAV Preps

    Type
    Collection
    ...Chemogenetics AAV Use our chemogenetics AAV to chemically induce neuronal activity in specific cell types...Gq-P2A-dTomato hM3D(Gq) - Activation dTomato (physically separate) none 1, 9, PHPeB Dimidschstein No available...

  16. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... a prime editing guide RNA (pegRNA). As with a typical gRNA, the pegRNA is designed with a spacer that...optimized for human codons, additional nuclear localization signals, and two mutations in Cas9 to enhance...

  17. TALEN Guide

    Type
    Collection
    ...activator-like (TAL) effectors, scientists in several biological disciplines are coming closer to this DNA targeting...DNA regions into a single construct would be technically challenging. Thanks to efforts by the Bogdanove...

  18. Viral Production

    Type
    Collection
    ... relative to empty particles (black arrowhead). Scale bar = 100 nm. Resources Browse AAV preps available...strain of E. coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification...

  19. Brain Armamentarium

    Type
    Collection
    ...(yellow fluorescent protein) in striatal and neocortical Sst-Chodl neurons Jonathan Ting Viviana Gradinaru...protein) in striatal Pvalb+ neurons. Note strong cortical Pvalb and L5 ET neuron expression. Jonathan Ting...

  20. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TACGAGGATCTGGTCCACtacggcatggatgcggTCGGGATCCCGTCTCCGA mical2b TAL3492 & TAL3493 TGGGGGAGACGGAGGAAGaacggacatctcaggcCGGTCAGCTTTTTGAGAA...TGCAGCCGTGTTTCTACCcggacgaggatgacttCTACTTCTGCAGACCAGA NADPH oxidase, EF-hand calcium binding domain 5 TAL3496 & TAL3497 TGCCGCCAACTGGCTGAAacctccagatctggagCAGAACAAGCGCAAAA...
Showing: 81 - 100 of 1006 results