We narrowed to 1,002 results for: Eras

...Expression mEmerald 487 509 39 6 11 min Monomer (A206K) mEmerald-N1 - Mammalian Expression mEmerald-C1 - Mammalian...Expression mPA-Emerald 487 509 400 Monomer (A206K) mPA-Emerald-N1 - Mammalian Expression mPA-Emerald-C1 - Mammalian... Mammalian Expression mEmerald-pBAD - Bacterial Expression EGFP 488 507 34 6 25 min Prone to dimerization...Michael Davidson Fluorescent Protein Collection Luciferase Plasmid Collection Promega Plasmid Collection...

...transduction of the trabecular meshwork following intracameral injection in mice, rats, and dogs. This serotype...serotype displays highly efficient transduction and lateral spread in the retina following subretinal injection...E531D) serotype has increased transduction and lateral spread in the retina following subretinal injection...Transduction optimization of AAV vectors for human gene therapy of glaucoma and their reversed cell entry characteristics...Display Exceptional Characteristics for Retinal Gene Therapy. Mol Ther . Jun 3;28(6):1464-1478. PMID: 32304666...

...resources for FRET experiments to measure protein interactions or conformation changes....FRET is often used to study (1) protein-protein interactions where each protein is separately fused to a ...proximity, affinity, and stoichiometry of protein interactions using image-based quantitative two-hybrid FRET...biosensors in Addgene's Biosensors Collection . Generate custom FRET-based biosensors with the cpFRET kit...Read more about self-labeling tags on our blog . Luciferase can be paired with a fluorescent protein acceptor...Resonance Energy Transfer (BRET) . Browse Addgene's Luciferase Plasmid Collection or the Promega Plasmid Collection...

...and throughput over traditional tagging methods. Several different tagging techniques, as well as the plasmids...plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt Unc-32::GFP targeting...Auxin-inducible degron (AID) tagging with CRISPR/Cas to generate conditional alleles of essential nuclear and cytoplasmic...technique for use in Drosophila cells that uses PCR to generate both an expression cassette for the Cas9-programming...cells 3.3 MB Seydoux C. elegans Tagging System Geraldine Seydoux's lab has developed a systematic and scalable...elegans . This system uses a 10-day protocol, generates “clean” homozygous mutants with no co-integrated...

...produced in-house by Addgene scientists. They are generated using standard methods individually optimized ...optimized for each specific antibody in order to generate high quality preparations. Following production, all...using ImageJ software and a standard curve is generated. The final antibody concentration is calculated...labeled with the antibody and a species-specific horseradish peroxidase (HRP)-conjugated secondary antibody...passed stability testing, it may ship at room temperature. However, Addgene determines ideal shipping conditions...

...their cellular machinery to generate viral vectors for research and gene therapy. Created with BioRender....can direct long-term transgene expression, but generally don't integrate into the host genome. AAVs are...Gamma-retroviral Stable RNA < 8 kb 80–130 nm Dividing Yes Moderate AAV Transient or Stable* Single-stranded linear...in the same way that wild-type viruses do. For general virus biosafety guidelines, check out Addgene's...

...plasmids to test whether or not you've efficiently generated infectious virus, plasmids expressing both the...process for packaging these transfer vectors and generating new viral vectors. Download the Lentivirus Transfer...coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP...72268 xpress gene of interest from the PGK (phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express... from pAdenoX from Clontech and can be used to generate adenovirus expressing a variety of reporters as...

...function, modeling diseases, and advancing gene therapy development. In order to produce recombinant AAV...guide for more information about AAV components, generations, viral production, and viral safety. AAV Vector...terminal repeats (ITRs). This table contains a general list of all the transfer plasmids in Addgene's ...specific packaging cell lines. Helper plasmids generally encode the adenoviral E4, E2a, and VA genes. This...Addgene's Biosafety Resources Addgene Protocols General Transfection AAV Production in HEK293T Cells AAV...

...James M. Wilson AV-1-PV0105 105532-AAV1 pAAV.CMV.ffLuciferase.SV40 Control James M. Wilson AV-1-PV1917 105541...James M. Wilson AV-8-PV0105 105532-AAV8 pAAV.CMV.ffLuciferase.SV40 Control James M. Wilson AV-8-PV0146 105535...Karl Deisseroth AV-5-PV0105 105532-AAV5 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-5-PV1090 105537-AAV5... M. Wilson AV-8-PV1302 105538-AAV8 pENN.AAV.TBG.PI.ffLuciferase.RBG James M. Wilson AV-8-PV3637 65015-...Karl Deisseroth AV-9-PV0105 105532-AAV9 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-9-PV0109 105533-AAV9...Karl Deisseroth AV-2-PV0105 105532-AAV2 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-1-PV3365 105554-AAV1...

...Experimental Considerations Recombinase Recognition Sites Each enzyme functions analogously but interacts only...molecules (in trans), a translocation event is generated between the target sites. Created with BioRender.com...such as Drosophila and mouse, particularly in proliferating cells. Expression may cause repetitive recombination...Use this AAV CRISPR knockout library expressing luciferase and Cre to interrogate tumorigenesis in vivo ...

...maintains a Standard Operating Procedure (Link opens in a new window) with general principles for working... in well-defined cellular sub-populations has generally been limited to single-component cellular definitions... cotransmission evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons. Elife... The central amygdala controls learning in the lateral amygdala. Nat Neurosci. 20(12):1680-1685. PubMed...

...expectations communicated by email will supersede the general guarantees above. We will have our expert viral...Production of a Packaged on Request material is generally ready for shipment within 8 weeks after the approval...queue is too high and we do not expect to meet the general turnaround time, Addgene will notify you by email...expectations communicated by email will supersede the general estimated turnaround time above. After starting...

...proteins then express and process the CRISPR loci to generate CRISPR RNAs (crRNAs). Through sequence homology...or repression. Combined with the power of next-generation sequencing, CRISPR is a robust system for genome-wide...RNA. Inosine is translated as guanosine, so the overall effect is an A->G change. Since this method targets...technologies. Biotechnology companies are exploring therapeutic applications of CRISPR to treat genetic disease...ethics associated with CRISPR in agricultural and therapeutic applications. Despite the ethical controversies...

...animals or hybridoma cells, but are difficult to generate due to expense and time. An alternative approach...fragment libraries from chickens Methods for the generation of chicken monoclonal antibody fragments by phage... 2015 Oct;167(2):95-102. Joanna Bereta Sybody Generation Toolbox for making synthetic nanobodies (sybodies...membrane proteins. Elife. 2018 May 24;7. and Generation of synthetic nanobodies against delicate proteins...

...individually. CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm...Jorgensen Lab. This kit contains 29 plasmids used to generate transgenic nematodes, including C. elegans and...Christian Frøkjær-Jensen's Wormbuilder website. General Tools Fire Lab C. elegans Vector Kit - Andrew Fire...plasmids - Paul Sternberg Lab. Plasmids for the temperature-robust GAL4-UAS system in C. elegans . Synthetic...

... trusting science in a “fake news and post-truth era,” the politicization of science (from climate change...intellectual prowess and scientific skills. This is an era of social media and self-promotion, and personality...contributed to the steadily increasing distrust the general public has in science and scientists (as discussed...have addressed CRISPR frequently over the last several years, but lay readers may be less familiar with... interesting case studies, and Zimmer provides several. In Part Three of the book, called simply “Old ...

...Potential applications for gene and cell-based therapies in the new era of personalized medicine (2017). ...Potential applications for gene and cell-based therapies in the new era of personalized medicine." Genes & diseases...nucleocapsid. B) Three generations of adenoviral vectors: the first generation vectors are deficient in...in trans. The Gutless AdV have generated high interest for gene therapy due their increased cloning capacity...attractive vectors for research applications and gene therapy: they can be produced at high titers, can accommodate...the Adv genome’s utility in research and its therapeutic potential. Note: constructs discussed are based...based on Adenovirus type 5. Three generations of adenoviral vectors The adenovirus virion is a non-enveloped...

...vision to accelerate scientific discovery that will help create new and more effective therapies for these...collaborative NET research community is pivotal in accelerating progress in the diagnosis and treatment of neuroendocrine...

...plasmids to express SARS-CoV-2 viral proteins in several expression systems....window) Researchers developing novel diagnostics, therapeutics, and vaccines against COVID-19 all rely on DNA...researchers to express SARS-CoV-2 viral proteins in several expression systems. Addgene partnered with Ginkgo...

...tool to control neuronal activity, scientists generally attach a fluorescent protein to the opsin in order... of proteins can also be controlled by light, generally by utilizing a light sensitive protein from cryptochromes...used as an optical switch to control protein interactions, dimerization, localization or activity of many...