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We narrowed to 1,000 results for: Eras

Showing: 81 - 100 of 1000 results

  1. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key cellular ... stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited here with Addgene...With these plasmids researchers will be able to generate fluorescently tagged proteins of key cellular ...Microtubules 101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase 1 Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP ...164500 POLR2A-mEGFP AICSDP-117 mEGFP RPB1 RNA polymerase II 168798 TERF2-mEGFP AICSDP-77 mEGFP Telomeric...CTCF-binding sites 170818 PCNA-mEGFP AICSDP-116 mEGFP Proliferating cell nuclear antigen (PCNA) DNA replication ...Protocols The Allen Institute for Cell Science has several protocol videos available that may be helpful for...

  2. TALEN Guide

    Type
    Collection
    ... activator-like (TAL) effectors, scientists in several biological disciplines are coming closer to this...fingers recognize triplets and the FokI nuclease operates as a dimer, cutting in the spacer region between...triplet has a corresponding zinc finger, and interactions between zinc fingers within an array can reduce...zinc fingers, potential targetable sites in your average genome are every 500 bp. TAL effectors have some...history. Dr. Keith Joung’s lab at Massachusetts General Hospital also recently released a TALEN kit through... Zinc Finger Consortium with Dr. Voytas, has considerable experience in the DNA targeting field. A third...specific gene or your anxiously investigating gene therapy options for a rare disease in humans, TAL effectors...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...to LacZ, plus luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase...For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 KB SpCas9 Plasmids The CRISPR...sgRNAs targeting KRAS , p53 , and LKB1 , plus luciferase-2A-Cre recombinase, and Kras G12D HDR template...of a single AAV vector ( AAV-KPL ) in the lung generated loss-of-function mutations in p53 and Lkb1 , as...plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase and sgRNAs targeting the mouse...plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase and an sgRNA targeted to LacZ...plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase and an sgRNA backbone for ...

  4. Viral Production

    Type
    Collection
    ...Resources Overview of Viral Production Viruses are generated using standard methods that have been optimized...optimized for each specific virus in order to generate high quality preparations. After production, all virus...the University of Pennsylvania Vector Core. In general, titering is performed by the facility that produced...suggests that titers obtained using ddPCR are generally 2–3 fold higher than those achieved using the ...us . Full sequencing of the Viral Genome Next-generation sequencing is performed on viral genomes isolated...preparations are produced in 293T cells using the 2nd generation psPAX2 and pMD2.G packaging system developed ...dilutions of the lentiviral vector, harvesting cells several days later, and isolating genomic DNA. This DNA...

  5. Ras Pathway

    Type
    Collection
    ...Class I PIN1 Peptidylprolyl cis/trans isomerase, NIMA-interacting 1 PLXNB1 Plexin B1 PPP1CA Protein phosphatase...relationship. The content and map for this page were generated with the help of Dominic Esposito and the Frederick... related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral...protein 2 ICMT Isoprenylcysteine carboxyl methyltransferase INSR Insulin receptor INSRR Insulin receptor-related... . Targeting RAS signalling pathways in cancer therapy. Downward J. Nat Rev Cancer. 2003 Jan;3(1):11-22...

  6. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...transduction of the trabecular meshwork following intracameral injection in mice, rats, and dogs. This serotype...serotype displays highly efficient transduction and lateral spread in the retina following subretinal injection...E531D) serotype has increased transduction and lateral spread in the retina following subretinal injection...Transduction optimization of AAV vectors for human gene therapy of glaucoma and their reversed cell entry characteristics...Display Exceptional Characteristics for Retinal Gene Therapy. Mol Ther . Jun 3;28(6):1464-1478. PMID: 32304666...

  7. CRISPR Plasmids - Tagging

    Type
    Collection
    ...and throughput over traditional tagging methods. Several different tagging techniques, as well as the plasmids...plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt Unc-32::GFP targeting...Auxin-inducible degron (AID) tagging with CRISPR/Cas to generate conditional alleles of essential nuclear and cytoplasmic...technique for use in Drosophila cells that uses PCR to generate both an expression cassette for the Cas9-programming...cells 3.3 MB Seydoux C. elegans Tagging System Geraldine Seydoux's lab has developed a systematic and scalable...elegans . This system uses a 10-day protocol, generates “clean” homozygous mutants with no co-integrated...

  8. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...TALEN array for genomic engineering Luciferase Reporter See our Luciferase collection of plasmid backbones... to create a luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase ...target genes can be inserted into this firefly luciferase reporter to test for their effects on protein...from an hU6 and an mU6 promoter Protein-protein interaction Proximity-dependent biotin identification (BioID...

  9. Retrovirus Plasmids

    Type
    Collection
    ...18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also expresses luciferase Lowe 9044 pMIG...gamma-retrovirus) is an RNA virus comprised of its genome and several structural and enzymatic proteins, including reverse...virus uses the reverse transcriptase in tow to generate a DNA provirus. This provirus then gets integrated...Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in mammalian cells Wong Return to Top Do you ...

  10. Antibody Production

    Type
    Collection
    ...produced in-house by Addgene scientists. They are generated using standard methods individually optimized ...optimized for each specific antibody in order to generate high quality preparations. Following production, all...using ImageJ software and a standard curve is generated. The final antibody concentration is calculated...labeled with the antibody and a species-specific horseradish peroxidase (HRP)-conjugated secondary antibody...passed stability testing, it may ship at room temperature. However, Addgene determines ideal shipping conditions...

  11. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...plasmids to test whether or not you've efficiently generated infectious virus, plasmids expressing both the...process for packaging these transfer vectors and generating infectious virus. Protocol: Lentivirus Transfer...coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP...72268 xpress gene of interest from the PGK (phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express... from pAdenoX from Clontech and can be used to generate adenovirus expressing a variety of reporters as...

  12. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...James M. Wilson AV-1-PV0105 105532-AAV1 pAAV.CMV.ffLuciferase.SV40 Control James M. Wilson AV-1-PV1917 105541...James M. Wilson AV-8-PV0105 105532-AAV8 pAAV.CMV.ffLuciferase.SV40 Control James M. Wilson AV-8-PV0146 105535...Karl Deisseroth AV-5-PV0105 105532-AAV5 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-5-PV1090 105537-AAV5... M. Wilson AV-8-PV1302 105538-AAV8 pENN.AAV.TBG.PI.ffLuciferase.RBG James M. Wilson AV-8-PV3637 65015-...Karl Deisseroth AV-9-PV0105 105532-AAV9 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-9-PV0109 105533-AAV9...Karl Deisseroth AV-2-PV0105 105532-AAV2 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-1-PV3365 105554-AAV1...

  13. Deisseroth INTRSECT Collection

    Type
    Collection
    ...maintains a Standard Operating Procedure (Link opens in a new window) with general principles for working... in well-defined cellular sub-populations has generally been limited to single-component cellular definitions... cotransmission evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons. Elife... The central amygdala controls learning in the lateral amygdala. Nat Neurosci. 20(12):1680-1685. PubMed...

  14. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...proteins then express and process the CRISPR loci to generate CRISPR RNAs (crRNAs). Through sequence homology...or repression. Combined with the power of next-generation sequencing, CRISPR is a robust system for genome-wide...RNA. Inosine is translated as guanosine, so the overall effect is an A->G change. Since this method targets...technologies. Biotechnology companies are exploring therapeutic applications of CRISPR to treat genetic disease...ethics associated with CRISPR in agricultural and therapeutic applications. Despite the ethical controversies...

  15. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...expectations communicated by email will supersede the general guarantees above. We will have our expert viral...Production of a Packaged on Request material is generally ready for shipment within 8 weeks after the approval...queue is too high and we do not expect to meet the general turnaround time, Addgene will notify you by email...expectations communicated by email will supersede the general estimated turnaround time above. After starting...

  16. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...amplify the pooled library and the use of Next-Generation Sequencing (NGS) to confirm library representation...Filters Name ID Library Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson DNA Repair...Libraries 213927, 213928 Knockout Cow Tan 2nd 4 on average 94,000 Bradley Human CRISPR Poison Exon Knockout...aeruginosa X. Liu NA 1 5,981 (Set 1) 5,971 (Set 2) CHyMErA Paralog & Dual-targeting hgRNA pooled library 155199...Knockout Human Moffat Blencowe 3rd variable 92,746 CHyMErA Exon-deletion hgRNA pooled library 155200 Knockout...

  17. Adenovirus Plasmids

    Type
    Collection
    ... viral capsid. Adenoviruses can be produced by several methods, the most common of which involves homologous...transfected into mammalian packaging cell lines to generate adenovirus particles. Read our Adenoviral Guide...terms Adenoviral Strains Bacterial strains for generating adenovirus. ID Strain Description PI 16398 BJ5183...for seamless assembly of multiple mutations, alterations, or transgene insertions into single full-length...

  18. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...an indel or inversion mutation [3]. In the CRISPR era, the genomic landscape may be surveyed in unprecedented...present a video reprint of the CRISPR article "Generation of Genomic Deletions in Mammalian Cell Lines ...between deletion size and frequency [3]. There are several applications in which the production of deletions...within the deletion boundaries may be produced to generate clonal allelic series. Single DSBs resolved by...

  19. Nuclear Receptor Signaling Atlas (NURSA) Plasmid Guide: Nuclear Receptors

    Type
    Collection
    ...receptors are: 1) a ligand binding domain, which interacts with hormones or other molecules, and 2) a DNA....5, NR1C1, PPAR, PPARalpha, hPPAR peroxisome proliferator-activated receptor alpha PPARD Plasmids PPARD..., NR1C2, NUC1, NUCI, NUCII, PPARB peroxisome proliferator-activated receptor delta PPARG Plasmids PPARG... NR1C3, PPARG1, PPARG2, PPARgamma peroxisome proliferator-activated receptor gamma RARA Plasmids RARA,...

  20. Antibody Plasmid Collection

    Type
    Collection
    ...animals or hybridoma cells, but are difficult to generate due to expense and time. An alternative approach...fragment libraries from chickens Methods for the generation of chicken monoclonal antibody fragments by phage... 2015 Oct;167(2):95-102. Joanna Bereta Sybody Generation Toolbox for making synthetic nanobodies (sybodies...membrane proteins. Elife. 2018 May 24;7. and Generation of synthetic nanobodies against delicate proteins...
Showing: 81 - 100 of 1000 results