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We narrowed to 795 results for: Tec

Showing: 81 - 100 of 795 results

  1. Synthetic Biology - Overview

    Type
    Collection
    ...synthetic biologists SEVA - Standard European Vector Architecture Open Plant - Plant SynBio resource EraSynBio...

  3. Video Library

    Type
    Protocol
    ...tips for mammalian cell culture Aseptic Technique Aseptic technique is a critical skill for success in numerous... the key strategies of aseptic technique, including personal protective equipment, setting up a clean ...Ultracentrifugation Protocol Mulitchannel Pipetting Technique Learn how to pipette multiple samples at once ...preparation. AAV Titration by qPCR Using SYBR Green Technology Protocol Agarose Gel Electrophoresis Gel electrophoresis...purify the DNA samples. This is a commonly used technique for molecular cloning, such as PCR- or restriction... process when requesting samples from Addgene Technology Transfer How to Order A step-by-step walkthrough...

  4. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ... within this set are derived from pAdenoX from Clontech and can be used to generate adenovirus expressing...

  5. Viral Vectors

    Type
    Collection
    ...genomes have been adapted into a plasmid-based technology, such that plasmids can be used to create viruses...

  6. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...Video Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs Learn how to best protect yourself when...Separate and detect specific proteins Watch the Video! Immunocytochemistry Use antibodies to detect antigens...questions. Intro to the Lab Bench Introductory techniques designed to help you get started in the lab. ...Video! AAV Titration by qPCR Using SYBR Green Technology Use qPCR to measure the physical titer of your...

  8. Open Enzyme Collection

    Type
    Collection
    ...immunology, biochemistry, and more. The foundational technologies enabled by this collection include polymerase...

  9. Brain Armamentarium

    Type
    Collection
    ...laboratory animals and human tissue specimens. These technologies have the potential to transform neuroscience...

  10. Ras Pathway

    Type
    Collection
    ...use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of RAS pathway gene...

  11. CRISPR References and Information

    Type
    Collection
    ...genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology. Developed by the Xiaole Shirley Liu Lab . For...

  12. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...purify the DNA samples. This is a commonly used technique for molecular cloning, such as PCR - or restriction... to an open UV box (be sure to wear proper UV protection - especially for your eyes!), remove it from ...the desired DNA fragment from the gel. Notes: To protect the UV box, it is a good idea to place the gel ...will not significantly reduce the UV, but will protect the UV box from being cut by the razor blade. Try...

  13. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...between different types of hazards and to provide protective measures for each level, as each level has different...Equipment Lab coat Close-toed shoes Gloves Eye protection or face shields Eye wash Safety shower Sharps... entering the lab, put on the proper personal protective equipment (PPE) and wear it the whole time you...re doing your work in the safest way possible. Protecting yourself and working smart in the lab is important...

  14. Immunocytochemistry

    Type
    Protocol
    ...Introduction Immunocytochemistry is a technique that uses antibodies to detect antigens in cells. Here we describe...µL of the 5 mg/mL DAPI solution to 100 µL PBS. Protect from light. Prepare a 300 nM DAPI working solution... the 300 µM DAPI stock solution into 5 mL PBS. Protect from light. Procedure Section 1: Seeding cells ...

  15. Western Blot

    Type
    Protocol
    ...Western blot is a technique used to separate proteins by size followed by detection using antibodies specific...gel imager with chemiluminescence detection or a dark room to detect the bands. Tips and Troubleshooting...

  17. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...Bacterial Transformation Summary The following technique can be used to easily move any piece of DNA from... by restriction digest is a commonly used lab technique. For the purposes of this tutorial we will discuss...from one plasmid to another. However, the same technique can be used to move promoters, selectable markers...

  18. Validated gRNA Sequences

    Type
    Collection
    ...GAGTGGGAGGGTCCCGTCCT 68060 cut S. pyogenes Fungal Biology and Biotechnology 2015, 2:4 Hong Ctnnb1 M. musculus AGCTCCTTCCCTGAGTGGCA...

  19. Brain Initiative Collection

    Type
    Collection
    ...Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative aims to revolutionize...

  20. Weighing Reagents Protocol

    Type
    Protocol
    ...for a stock mixture is an essential laboratory technique, as imprecise measurements can affect the outcome...weigh reagents. Wear the appropriate personal protective equipment (PPE) prior to handling the materials...
Showing: 81 - 100 of 795 results