...dividing cells. CRISPR Activate and Interfere technologies also indirectly modify local chromatin state...

...activator system - Jaenisch Locus-specific ChIP technologies (iChIP & enChIP) using dCas9 - H. Fujii Disease...

...use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of RAS pathway gene...

...biosensors. Optogenetics Plasmids | Guide Use light to detect, measure, and control molecular signals, cells,...

...split fluorescence complementation probe used for detecting protein-protein interactions in vitro and in vivo...

... CRISPR Plasmids and Resources CRISPR technologies are continously developing and expanding. Addgene...

...constructs and routine protein and molecular biology techniques. Using a single protein with many available isoforms...

...purify the DNA samples. This is a commonly used technique for molecular cloning, such as PCR - or restriction... to an open UV box (be sure to wear proper UV protection - especially for your eyes!), remove it from ...the desired DNA fragment from the gel. Notes: To protect the UV box, it is a good idea to place the gel ...will not significantly reduce the UV, but will protect the UV box from being cut by the razor blade. Try...

...synthetic biologists SEVA - Standard European Vector Architecture Open Plant - Plant SynBio resource EraSynBio...

...such as MoClo . Check out our Molecular Cloning Techniques Guide for an overview of the most common methods...

...between different types of hazards and to provide protective measures for each level, as each level has different...Equipment Lab coat Close-toed shoes Gloves Eye protection or face shields Eye wash Safety shower Sharps... entering the lab, put on the proper personal protective equipment (PPE) and wear it the whole time you...re doing your work in the safest way possible. Protecting yourself and working smart in the lab is important...

...Introduction Immunocytochemistry is a technique that uses antibodies to detect antigens in cells. Here we describe...µL of the 5 mg/mL DAPI solution to 100 µL PBS. Protect from light. Prepare a 300 nM DAPI working solution... the 300 µM DAPI stock solution into 5 mL PBS. Protect from light. Procedure Section 1: Seeding cells ...

...Western blot is a technique used to separate proteins by size followed by detection using antibodies specific...gel imager with chemiluminescence detection or a dark room to detect the bands. Tips and Troubleshooting...

... within this set are derived from pAdenoX from Clontech and can be used to generate adenovirus expressing...

...genomes have been adapted into a plasmid-based technology, such that plasmids can be used to create viruses...

...somatic hypermutation in a 100 bp window. This technique creates diverse populations of mutants for directed...

...immunology, biochemistry, and more. The foundational technologies enabled by this collection include polymerase...

...laboratory animals and human tissue specimens. These technologies have the potential to transform neuroscience...

...use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of RAS pathway gene...

...CRISPR in your lab. Learn More Guide to CRISPR technology Addgene Blog Posts How to Design Your gRNA for...