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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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In Category

We narrowed to 797 results for: Tec

Showing: 81 - 100 of 797 results

  1. Ras Pathway

    Type
    Collection
    ...use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of RAS pathway gene...

  2. Fluorescent Proteins: FRET

    Type
    Collection
    ...microscopy or spectroscopy by exciting the donor and detecting the emission of the acceptor. Alternatively, the...

  3. CRISPR References and Information

    Type
    Collection
    ...genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology. Developed by the Xiaole Shirley Liu Lab . For...

  4. Brain Armamentarium

    Type
    Collection
    ...laboratory animals and human tissue specimens. These technologies have the potential to transform neuroscience...

  6. Video Library

    Type
    Protocol
    ...tips for mammalian cell culture Aseptic Technique Aseptic technique is a critical skill for success in numerous... the key strategies of aseptic technique, including personal protective equipment, setting up a clean ...Ultracentrifugation Protocol Mulitchannel Pipetting Technique Learn how to pipette multiple samples at once ...preparation. AAV Titration by qPCR Using SYBR Green Technology Protocol Agarose Gel Electrophoresis Gel electrophoresis...purify the DNA samples. This is a commonly used technique for molecular cloning, such as PCR- or restriction... process when requesting samples from Addgene Technology Transfer How to Order A step-by-step walkthrough...

  7. Validated gRNA Sequences

    Type
    Collection
    ...GAGTGGGAGGGTCCCGTCCT 68060 cut S. pyogenes Fungal Biology and Biotechnology 2015, 2:4 Hong Ctnnb1 M. musculus AGCTCCTTCCCTGAGTGGCA...

  8. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...Video Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs Learn how to best protect yourself when...Separate and detect specific proteins Watch the Video! Immunocytochemistry Use antibodies to detect antigens...Antibodies Intro to the Lab Bench Introductory techniques designed to help you get started in the lab. ...Video! AAV Titration by qPCR Using SYBR Green Technology Use qPCR to measure the physical titer of your...

  9. Brain Initiative Collection

    Type
    Collection
    ...Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative aims to revolutionize...

  10. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...purify the DNA samples. This is a commonly used technique for molecular cloning, such as PCR - or restriction... to an open UV box (be sure to wear proper UV protection - especially for your eyes!), remove it from ...the desired DNA fragment from the gel. Notes: To protect the UV box, it is a good idea to place the gel ...will not significantly reduce the UV, but will protect the UV box from being cut by the razor blade. Try...

  11. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...between different types of hazards and to provide protective measures for each level, as each level has different...Equipment Lab coat Close-toed shoes Gloves Eye protection or face shields Eye wash Safety shower Sharps... entering the lab, put on the proper personal protective equipment (PPE) and wear it the whole time you...re doing your work in the safest way possible. Protecting yourself and working smart in the lab is important...

  12. Immunocytochemistry

    Type
    Protocol
    ...Introduction Immunocytochemistry is a technique that uses antibodies to detect antigens in cells. Here we describe...µL of the 5 mg/mL DAPI solution to 100 µL PBS. Protect from light. Prepare a 300 nM DAPI working solution... the 300 µM DAPI stock solution into 5 mL PBS. Protect from light. Procedure Section 1: Seeding cells ...

  13. Western Blot

    Type
    Protocol
    ...Western blot is a technique used to separate proteins by size followed by detection using antibodies specific...gel imager with chemiluminescence detection or a dark room to detect the bands. Tips and Troubleshooting...

  14. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...Bacterial Transformation Summary The following technique can be used to easily move any piece of DNA from... by restriction digest is a commonly used lab technique. For the purposes of this tutorial we will discuss...from one plasmid to another. However, the same technique can be used to move promoters, selectable markers...

  15. Weighing Reagents Protocol

    Type
    Protocol
    ...for a stock mixture is an essential laboratory technique, as imprecise measurements can affect the outcome...weigh reagents. Wear the appropriate personal protective equipment (PPE) prior to handling the materials...

  16. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...insert and vector backbone are known and thus this technique can be quickly used to verify your plasmid. The... should be. A useful restriction enzyme based technique for verifying plasmids like this is "plasmid fingerprinting...

  17. Centrifugation

    Type
    Protocol
    ...Microfuge tubes or other suitable container Personal protective equipment (PPE) appropriate for the biosafety...higher speeds and are used for more specialized techniques. Regardless of the type of centrifuge you use...

  18. Gibson Assembly Protocol

    Type
    Protocol
    ...ET SSB) to the isothermal reaction mix. ET SSB protects 3’ ssDNA ends from the ssDNA-specific endonuclease... is to “stitch” together several oligos. This technique is especially useful for introducing promoters...

  19. Using a Light Microscope Protocol

    Type
    Protocol
    ...advantage of the physical properties of light to detect small objects. Two of the most important properties...Procedure Set up your microscope by removing any protective cover that may be on it and plugging in the power...

  20. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...EtBr is a known mutagen. Wear a lab coat, eye protection and gloves when working with this chemical. Note... damage to the DNA. Note: When using UV light, protect your skin by wearing safety goggles or a face shield...
Showing: 81 - 100 of 797 results