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... Return to Top Other Tet Applications Explore some other popular applications of tetracycline systems ...powerful tools to control gene expression and study biological targets. The tetracycline (tet) system is one...improvements. A synthetic Tet Response Element (TRE) typically features seven copies of tet O upstream of a minimal... to a reverse Tet repressor, or rTetR, which specifically binds the TRE in the presence of tetracycline...for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative Doxycycline...vectors for transactivators, and vectors for other applications, or search our collection for all dox-regulated...pAAV-Ptet-RFP-shR-rtTA AAV Tet-On shRNA vector. To evaluate shRNA efficacy by fluorescence, use Plasmid #35626 as a reporter...

...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...distributes cannot be used to reconstitute self-replicating microbes or recreate the diseases they cause....our Search page to look for specific genes or applications that are not listed here. Addgene Microbiology...Prions Plasmids for Protozoa Species Entamoeba histolytica Leishmania sp. Plasmodium sp. Toxoplasma gondii...vaccinia virus - Bennink and Yewdell Labs Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly...transcription units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors...fluorescent protein plasmid collection is organized by application and by color. External Resources European Saccharomyces...

...with most experiments, you first need to have a biological question in mind. Then you can decide how to ...component for each category. Genetic Manipulation Application Cas Enzyme Additional Considerations Browse Plasmids...gene you target will depend on your specific application. Knockout : Target constitutively expressed regions...protein and gRNA, which you generally perform by chemical transfection or electroporation. Expression can...systems and delivery methods . Validate Genetic Modification Once you have successfully delivered the gRNA...questions: Does the resulting population need to be identical (clonal) or is a mixed population okay? Does the...the edit need to be homozygous? What type of modification is it? Are you modifying the genomic sequence...

...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...Marker PI Publication Return to top C. elegans ID Plasmid Gene/Insert Promoter PI Publication Return to...Drosophila Plant C. elegans Yeast Resources Catalytically dead dCas9 fused to a transcriptional activator...Drosophila ID Plasmid Gene/Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a ...

...synthetic biology by providing access to new chemical and physical properties for studying, manipulating, and...incorporation of non-canonical amino acids (ncAA) in a protein of interest. Applications By making small changes...acids beyond the 20 standard ones, known as non-canonical amino acids (ncAAs). This strategy, called genetic...a ncAA into a protein of interest, four key modifications to the standard translation machinery are required...reassigned codon. An aaRS enzyme is engineered to specifically load the new tRNA with the ncAA. Together, the...with a different amino acid. To achieve these modifications, two plasmids are generally used. Once these...that contain genes with modified codons for non-canonical amino acid incorporation. ID Plasmid Gene/Insert...

...editors are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase...and are thus well suited to directed evolution applications. Examples of these base editing systems include...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable...Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI...PI Publication Return to top Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...

... of stochastic combinations of cell-filling antigenically distinct protein tags (or "bits"), creating ...components can be used separately for additional applications. Park & Sheridan et al. (2025) use plasmids ...circuit mapping and reconstruction. Potential applications could also include using individual or subsets...well as barcoding with specific enhancers for morphological readout. How to Use PRISM Barcodes are delivered...AAVs either directly to a region of interest or systemically using a capsid capable of crossing the blood-brain...microscopy workflows to achieve high-resolution optical imaging down to individual synapses. Figure 2: ...new window) , and The Max Planck Institute for Biological Intelligence (Link opens in a new window) . Read...

...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...and time. An alternative approach is to use synthetically produced recombinant antibodies, created by ...extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb...imaging. Learn more about antibodies and their applications in our Antibody Guide ! Cloning and Expressing...antibodies, and Fc-silent mutants. Functional diversification of hybridoma-produced antibodies by CRISPR/...456(1):527-33. Gavin Wright pET-30-based vector dedicated to efficient scFv expression, which circumvents...and Generation of synthetic nanobodies against delicate proteins. Nat Protoc. 2020 Apr 8. Markus Seeger...

...synthetic chemical ligands, which have been developed for special functions that vary from purification, visualization...Promega technologies that support a range of applications. The technology includes NanoLuc, HaloTag, SmBiT...useful for both intracellular and extracellular applications where you want minimal disruption of endogenous...levels in lysates or live-cells, thus preserving physiological relevance. NanoBRET Assays NanoBRET (Link opens...similar premise, but examine the proximity of a chemical compound and its target protein. The apparent ...Connections (Link opens in a new window) blogs for technical tips and tools for genetic reporter assays (Link...

...55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate mCherry expression Recombinases Karl...55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a Flpo and physically-separate mCherry expression Recombinases Karl...AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato Calcium sensor Jonathan...-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear dTomato, Cre-dependent Calcium...AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear dTomato Calcium sensor Jonathan... Loren Looger 113050 pAAV-hSyn-GRAB_DA1h Syn genetically-encoded fluorescent dopamine(DA) sensor GRAB_...sensor Yulong Li 123308 pAAV-hSyn-GRAB_NE1m Syn genetically-encoded fluorescent norepinephrine(NE) sensor...

... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned scarlessly into the vector...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned into the CRISPR array. The...- sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse the Addgene... genes. It consists of three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin...

...repository contains many plasmid tools that were specifically designed for use in plants. These resources ...Infrastructure Kit Modules connect MoClo and Gateway for hierarchical assembly of plant multigene constructs; contains...multi-purpose toolkit that enables targeted, specific modification of monocot and dicot genomes using TALENs and...CRISPR. Successfully validated in tomato, tobacco, Medicago truncatula , wheat, and barley. Return to top ...localization, protein–protein interactions, and other physiological processes in plants. ID Plasmid Description ...an Old Favorite External Resources Arabidopsis Biological Resource Center (ABRC) (Link opens in a new window...Taproot is a podcast to understand how scientific publications are created. Papers from the plant biology literature...

...Marker PI Publication Return to top Cut ID Plasmid Gene/Insert Selectable Marker PI Publication Return to...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Base Edit ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Nick ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter Selectable...Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR ...

... PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Base Edit ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Nick ID Plasmid Description Gene...Gene/Insert Promoter Selectable Marker PI Publication Return to top Purify ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of...

...antibodies. These monoclonal antibodies undergo application-specific validation and quality control by Addgene...antigen. Addgene supplies a list of recommended applications based on our in-house testing and data provided...with full experimental details in the Antibody Applications section of our product pages. We also include... an antibody’s use is not recommended for an application or species. We currently assess western blot,...scientists to further develop and refine these application lists. The plasmids we use to produce antibodies...Source Species Isotype Reactivity Recommended Applications PI Return to Top Do you have suggestions for...

...) domains that respond specifically to small molecules termed Pharmacologically Selective Effector Molecules...Plasmid Collection Chemogenetics plasmids encode genetically engineered receptors that are activated by specific... to artificially activate or inhibit specific biological processes. Addgene's collection contains two ...Activated by Designer Drugs (DREADDs) and Pharmacologically Selective Actuator Modules (PSAMs). DREADDs...have been engineered to respond to specific physiologically inert small molecules. There are four types...

...subject to Addgene’s Terms of Purchase and the applicable material transfer agreements (MTAs) and limited...without a guaranteed titer. Any titer expectations communicated by email will supersede the general guarantees...step of the QC process, viral genome sequence verification, may not yet have been completed at the time...longer turnaround time. Any timing expectations communicated by email will supersede the general estimated...be required by your country for the import of biological research materials. Please be aware that additional...contact you directly regarding these charges, if applicable....

... Tools for Affinity Purification These AAV encode tools for affinity purification (which can be used for...tet-off transactivators and tools for affinity purification (TRAP). Viral... Tools Tetracycline Transactivators Affinity Purification Neurophysiology Cell Ablation Genome Engineering...of the voltage-gated Na+ channel mNaChBac and (physically separate) tdTomato. 8 Massimo Scanziani 34910...constitutive Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP...

...horizontal stripes on the sides of the adult body, is a tropical freshwater fish in the minnow family. It has long...inflammation and infection, regeneration, human neurological disorders, microbiota studies, and drug discovery...Cell lineage tracing in multicellular systems typically relies on genetic markers, somatic mutations, ...types. CreLite - George Eisenhoffer Lab. An optogenetically controlled Cre/loxP system that enables precise...the international research community. The Latin American Zebrafish Network (LAZEN) - LAZEN aims to increase...advanced training of young scientists in Latin America. The Zebrafish Book - A guide for the laboratory...

...Brain Science AAV Enhancer Collection Plasmids Publications Resources Enhancer AAV plasmids are plasmids...following regions: Isocortex Hippocampal formation Cortical subplate Striatum Pallidum Hypothalamus Cerebellum...pAAV-AiE2051m-minBG-SYFP2-WPRE3-BGHpA AiP1277 AiE2051m SYFP2 Claustrum Cortical subplate 220711 pAAV-AiE2051m-minBG-iCre(R297T...R297T)-BGHpA AiP1786 AiE2051m Cre Claustrum Cortical subplate 230518 pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA ...pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA AiP1211 AiE2051m FlpO Claustrum Cortical subplate 191720 pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA ...= Substantial non-specific labeling observed Publications Ben-Simon, Y., Hooper, M., Narayan, S., Daigle...and transgenic mouse lines for genetic access to cortical cell types. Cell , 188 (11), 3045–3064. https:...