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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 799 results for: tec

Showing: 81 - 100 of 799 results

  2. CRISPR Plasmids and Resources

    Type
    Collection
    ... CRISPR Plasmids and Resources CRISPR technologies are continously developing and expanding. Addgene...

  4. Synthetic Biology - Overview

    Type
    Collection
    ...synthetic biologists SEVA - Standard European Vector Architecture Open Plant - Plant SynBio resource EraSynBio...

  6. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...Video Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs Learn how to best protect yourself when...Separate and detect specific proteins Watch the Video! Immunocytochemistry Use antibodies to detect antigens...questions. Intro to the Lab Bench Introductory techniques designed to help you get started in the lab. ...Video! AAV Titration by qPCR Using SYBR Green Technology Use qPCR to measure the physical titer of your...

  7. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ... within this set are derived from pAdenoX from Clontech and can be used to generate adenovirus expressing...

  8. Viral Vectors

    Type
    Collection
    ...genomes have been adapted into a plasmid-based technology, such that plasmids can be used to create viruses...

  10. Open Enzyme Collection

    Type
    Collection
    ...immunology, biochemistry, and more. The foundational technologies enabled by this collection include polymerase...

  11. Brain Armamentarium

    Type
    Collection
    ...laboratory animals and human tissue specimens. These technologies have the potential to transform neuroscience...

  12. Ras Pathway

    Type
    Collection
    ...use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of RAS pathway gene...

  13. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...purify the DNA samples. This is a commonly used technique for molecular cloning, such as PCR - or restriction... to an open UV box (be sure to wear proper UV protection - especially for your eyes!), remove it from ...the desired DNA fragment from the gel. Notes: To protect the UV box, it is a good idea to place the gel ...will not significantly reduce the UV, but will protect the UV box from being cut by the razor blade. Try...

  14. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...between different types of hazards and to provide protective measures for each level, as each level has different...Equipment Lab coat Close-toed shoes Gloves Eye protection or face shields Eye wash Safety shower Sharps... entering the lab, put on the proper personal protective equipment (PPE) and wear it the whole time you...re doing your work in the safest way possible. Protecting yourself and working smart in the lab is important...

  15. CRISPR References and Information

    Type
    Collection
    ...genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology. Developed by the Xiaole Shirley Liu Lab . For...

  16. Immunocytochemistry

    Type
    Protocol
    ...Introduction Immunocytochemistry is a technique that uses antibodies to detect antigens in cells. Here we describe...µL of the 5 mg/mL DAPI solution to 100 µL PBS. Protect from light. Prepare a 300 nM DAPI working solution... the 300 µM DAPI stock solution into 5 mL PBS. Protect from light. Procedure Section 1: Seeding cells ...

  17. Western Blot

    Type
    Protocol
    ...Western blot is a technique used to separate proteins by size followed by detection using antibodies specific...gel imager with chemiluminescence detection or a dark room to detect the bands. Tips and Troubleshooting...

  19. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...Bacterial Transformation Summary The following technique can be used to easily move any piece of DNA from... by restriction digest is a commonly used lab technique. For the purposes of this tutorial we will discuss...from one plasmid to another. However, the same technique can be used to move promoters, selectable markers...

  20. Validated gRNA Sequences

    Type
    Collection
    ...GAGTGGGAGGGTCCCGTCCT 68060 cut S. pyogenes Fungal Biology and Biotechnology 2015, 2:4 Hong Ctnnb1 M. musculus AGCTCCTTCCCTGAGTGGCA...
Showing: 81 - 100 of 799 results