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We narrowed to 130 results for: yeast expression

Showing: 81 - 100 of 130 results

  1. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...into a vector encoding a fluorescent tag. Once expressed as a fluorescent protein fusion, it is possible... For subcellular markers in yeast see our plasmid collection from Sue Jaspersen (Stowers...subcellular structure or protein complex. By coexpressing both the marker protein and the protein of interest...

  2. CRISPR Plasmids - Yeast

    Type
    Collection
    ... CRISPR Yeast CRISPR Plasmids: Yeast Browse CRISPR Plasmids By Function...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...have been designed for use in yeast. CRISPR... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...following CRISPR plasmids have been designed for use in yeast. Cut Fully functional CRISPR/Cas enzymes will introduce...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... expression pINDUCER21 (ORF-EG) - Inducible lentiviral gene expression vector URA3 Yeast pAG306GAL-ccdB... to express a gene; however, we have included a function section for if you want to stably express an ...genetic element, or want to express shRNA. Choose by: Species-Specific Expression Epitope Tag or Fusion Protein...Selectable Markers Species-Specific Expression If you want to drive expression of your favorite gene, you will...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent ...Empty mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag ... Inducible Expression page Bacteria Lac, T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector with...

  4. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Addgene Kits MoClo-YTK Yeast Gateway Kit Multiplex Yeast Toolkit (MYT) Markerless Yeast Localization and Overexpression... Depositor Collections Botman-Teusink Yeast FP Collection Botman-Teusink Yeast FP Collection... . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with yeast codon-optimized...Publication FP Overexpression Plasmids for constitutive overexpression of codon-optimized FPs in yeast. Vectors...Collection FP Tagging FP Overexpression Resources This comprehensive collection of yeast codon-optimized fluorescent...for constitutive overexpression of FPs and tagging of genes of interest with FPs in yeast. Characterization...proteins in yeast. Depositor...

  5. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...particular expression system , eg. Mammalian, Lentiviral, AAV, Bacteria, Drosophila, Yeast, Plant, Xenopus...by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI...Basta Kim pMZ283 52224 Yeast CspCI none S. pyogenes URA4 Zaratiegui pJZC588 62315 Yeast none S. pyogenes URA3...gRNA_handle_RPR1t 62966 Yeast HindIII none S. pyogenes Puro Lu pML104 67638 Yeast BclI-SwaI yes, cut S. ...Wyrick pML107 67639 Yeast BclI-SwaI yes, cut S. pyogenes LEU2 Wyrick pT040 67640 Yeast BclI-SwaI none S....Jackson pMZ376 74213 Yeast rrk1 yes, cut S. pyogenes KanMX Zaratiegui pMZ377 74214 Yeast rrk1 yes, cut S. ...can be used to express multiple gRNAs from a single plasmid with or without expression of Cas9. Highlighted...

  7. Modular Cloning Guide

    Type
    Guide
    ...constructs for S. cerevisiae expression. Multiplex Yeast Toolkit (MYT) Yeast Expression, CRISPR Tom Ellis 96 ...uLoop Assembly Kit Other, Yeast Expression, Plant Expression, Bacterial expression Christopher Dupont 95 ...transcription units in the oleaginous yeast Y. lipolytica . EXPRESSYALI Yeast Expression Irina Borodina Plasmids for...Aspergillus species. MoClo Yeast Secretion and Display (YSD) Toolkit Yeast Expression Paul Young 34 plasmids...display of proteins of interest. Yeast Secrete and Detect Yeast Expression Sylvestre Marillonnet Plasmids...secretion in yeasts S. cerevisiae and P. pastoris (a.k.a. K. phaffii ). POMBOX MoClo YTK Yeast Expression Tomáš...protein expression and secretion in P. pastoris (a.k.a. K. phaffii ). GoldenPiCS Kit Yeast Expression Brigitte...

  8. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Plasmids are available for expression in mammalian systems, bacteria, plants, and yeast. Mammalian ID Plasmid... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker...

  9. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the... available for expression in mammalian systems, bacteria, Drosophila, plants, and yeast. Mammalian ID ... plasmids for activating or upregulating gene expression in various model organisms. CRISPR... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ... ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker...

  10. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... effectively biotinylate AviTag expressing proteins in bacteria, yeast, insect and mammalian cells. Check...more on this below) it can be incubated or co-expressed with avidin or a structurally similar protein ...

  11. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Plasmids are available for expression in mammalian systems, bacteria, and yeast. Mammalian ID Plasmid Gene... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system...Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker...

  13. CRISPR Plasmids and Resources

    Type
    Collection
    ...designed for use in plants. Yeast CRISPR plasmids designed for use in yeast. Xenopus CRISPR plasmids designed...organisms, including mammals, plants, bacteria, yeast, Drosophila, zebrafish, C. elegans, parasites, and... fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor...epigenetic modifier alters chromatin structure/gene expression. RNA Targeting RNA Targeting Type VI CRISPR systems...

  14. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Plasmids are available for expression in mammalian systems, bacteria, plants, yeast, and zebrafish. Mammalian... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...Further advancements have been made by optimizing expression of the fusions, modifying the linker region between...Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker...

  15. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...bacterial expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT... SFFV) or inducible (CMV-TO) cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive (H1...enhancing target gene expression anywhere from 87x to 20,000x over endogenous expression (depending on the...Testing your cell-free expression system: There’s a kit for that! Cell-free expression systems have been ...comparison of different cell-free expression systems; the Cell-free expression test kit represents a set of...benchmarking of cell-free expression systems, and tested for product integrity, expression level, and aggregation...fragments facilitates target DNA cleavage upon co-expression and spontaneous self-assembly. In an effort to...

  16. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ... available for expression in mammalian systems, bacteria, Drosophila, plants, and yeast. Mammalian ID ... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Description Gene/Insert Promoter Selectable...

  17. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...available for expression in mammalian systems, bacteria, Drosophila, plants, C. elegans, yeast, zebrafish... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker...

  18. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...CRISPR-FokI plasmids. Plasmids are available for expression in mammalian systems and Drosophila. Mammalian... ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter PI Publication Do...

  20. Cloning

    Type
    Guide
    ...protocol ) Back to Top Yeast-mediated Cloning and Oligonucleotide Stitching Yeast-mediated cloning is very...them into plasmids for replication and protein expression. So how do scientists recombine DNA? There are...majority of plasmids used for DNA cloning and expression contain several popular restriction enzyme sites...advantage of the powerful recombination abilities of yeast. Similar to Gibson, this method can efficiently ...accomplish this, you just need to introduce into the yeast the two (or more) fragments of DNA that you would...be set up to grow, transform and purify DNA from yeast. Back to Top...
Showing: 81 - 100 of 130 results