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We narrowed to 124 results for: yeast expression

Showing: 81 - 100 of 124 results

  1. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ... multiple parts transcribed together. They can express one or more gene(s), and typically begin with a...strategies are not available to those working with yeast or plants. Using MoClo, or better yet, a pre-made...

  2. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...homology-based searches return no candidates. Our strategy: Express random fragments of the phage genome and probe ...plate) across 20 fresh selective plates. Step 2: Express random phage DNA fragments in E. coli Now that ...and exposed to IPTG in order to induce protein expression. Cells on the membranes will then be exposed ...sleeping) point for this experiment. Find Bacterial Expression Vectors in the FX Cloning System Kit  Step 3:... and growing them up overnight. Assuming they expressed well, the proteins encoded by the plasmids in ...the membrane itself or to any “sticky” proteins expressed by your library. After blocking for 1 h at room...at room temperature. This will allow any RBPs expressed on the membrane to bind their target cells, pulling...

  3. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...genes close to native expression levels. However, it is advisable to first express the fusion protein from...strategy (Sheridan, 2002). Avoiding protein over-expression artifacts The introduction of DNA that encodes...of protein within a cell and may lead to over-expression artifacts. This is an important downside of the...characterisation of fluorescent proteins in budding yeast." Scientific reports 9.1 (2019): 2234. PubMed PMID...

  4. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...spread exclusively through populations that already express Cas9 from an unlinked locus, while a Cas9+sgRNA...efficiently and reversibly bias inheritance in wild yeast. bioRxiv 013896 (2015). doi:10.1101/013896. bioRxiv...methods are easy to implement and have been tested in yeast5. Ecological confinement involves performing experiments...

  5. CRISPR Plasmids and Resources

    Type
    Collection
    ...designed for use in plants. Yeast CRISPR plasmids designed for use in yeast. Xenopus CRISPR plasmids designed...organisms, including mammals, plants, bacteria, yeast, Drosophila, zebrafish, C. elegans, parasites, and... fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor...epigenetic modifier alters chromatin structure/gene expression. RNA Targeting RNA Targeting Type VI CRISPR systems...

  6. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the... plasmids for activating or upregulating gene expression in various model organisms. CRISPR... Mammalian Bacteria Drosophila Plant C. elegans Yeast Resources Catalytically dead dCas9 fused to a transcriptional...Gene/Insert Promoter PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker...

  7. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...CRISPR Plasmids: Interfere Mammalian Bacteria Plant Yeast Resources Catalytically dead Cas9 (dCas9), or dCas9...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. This interference... Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker...

  8. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...into a vector encoding a fluorescent tag. Once expressed as a fluorescent protein fusion, it is possible... For subcellular markers in yeast see our plasmid collection from Sue Jaspersen (Stowers...subcellular structure or protein complex. By coexpressing both the marker protein and the protein of interest...

  9. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...CRISPR-FokI plasmids. Plasmids are available for expression in mammalian systems and Drosophila. Mammalian... ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter PI Publication Do...

  11. Modular Cloning Guide

    Type
    Guide
    ...constructs for S. cerevisiae expression. Multiplex Yeast Toolkit (MYT) Yeast Expression, CRISPR Tom Ellis 96 ...uLoop Assembly Kit Other, Yeast Expression, Plant Expression, Bacterial expression Christopher Dupont 95 ...transcription units in the oleaginous yeast Y. lipolytica . EXPRESSYALI Yeast Expression Irina Borodina Plasmids for...Aspergillus species. MoClo Yeast Secretion and Display (YSD) Toolkit Yeast Expression Paul Young 34 plasmids...display of proteins of interest. Yeast Secrete and Detect Yeast Expression Sylvestre Marillonnet Plasmids...secretion in yeasts S. cerevisiae and P. pastoris (a.k.a. K. phaffii ). POMBOX MoClo YTK Yeast Expression Tomáš...protein expression and secretion in P. pastoris (a.k.a. K. phaffii ). GoldenPiCS Kit Yeast Expression Brigitte...

  12. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... effectively biotinylate AviTag expressing proteins in bacteria, yeast, insect and mammalian cells. Check...more on this below) it can be incubated or co-expressed with avidin or a structurally similar protein ...

  13. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ... CRISPR: Purify Genomic Loci Mammalian Bacteria Yeast Resources Identifying molecules associated with ...fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system... Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker...

  15. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...Ginkgo Bioworks plasmids to express SARS-CoV-2 viral proteins in several expression systems.... enable researchers to express SARS-CoV-2 viral proteins in several expression systems. Addgene partnered...nsp3, OC43, NL63, HKU1, and 229E) Expression type (e.g. bacterial, yeast, or mammalian) Tags (e.g. 6xHis...to Industry page. These plasmids have not been expressed or functionally validated by Ginkgo or Addgene... V5) Mutations Wild type (wt) Codon-optimized expression (recode) Truncated for making pseudovirus (Ctrunc...Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems Collection: COVID-19 and Coronavirus ...

  16. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...CRISPR Plasmids: Base Edit Mammalian Bacteria Plant Yeast Zebrafish Resources Two classes of base editors ...Further advancements have been made by optimizing expression of the fusions, modifying the linker region between... Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker...

  17. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...bacterial expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT... SFFV) or inducible (CMV-TO) cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive (H1...enhancing target gene expression anywhere from 87x to 20,000x over endogenous expression (depending on the...Testing your cell-free expression system: There’s a kit for that! Cell-free expression systems have been ...comparison of different cell-free expression systems; the Cell-free expression test kit represents a set of...benchmarking of cell-free expression systems, and tested for product integrity, expression level, and aggregation...fragments facilitates target DNA cleavage upon co-expression and spontaneous self-assembly. In an effort to...

  18. Depositor Collections

    Type
    Collection
    ...Plasmid Collection Botman-Teusink Yeast FP Collection Protein Expression Cell Migration Consortium Malate...to drive selective expression in the brain - Simpson Reagents for improved expression of recombinant phosphoproteins...CRISPR, disease research, visualization, protein expression, synthetic biology, and viral vector plasmids...Open Enzyme Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection Synthetic...

  19. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...break (DSBs) at a specific location based on a co-expressed gRNA-defined target sequence. DSBs are preferentially...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  20. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...
Showing: 81 - 100 of 124 results