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We narrowed to 1,149 results for: Gene

Showing: 1121 - 1140 of 1149 results

  1. Antibody Production

    Type
    Collection
    ...distributed by Addgene have been produced in-house by Addgene scientists. They are generated using standard...or by contacting help@addgene.org . Some of the antibodies distributed by Addgene were developed by the...Overview of Addgene's antibody production methods and quality control processes for purified recombinant...optimized for each specific antibody in order to generate high quality preparations. Following production...titered and undergo stringent quality control by Addgene or a trusted quality control partner before distribution... preparations developed at IPI may differ from Addgene antibodies in “Production & Usage” and “Delivery...using ImageJ software and a standard curve is generated. The final antibody concentration is calculated...

  2. Caltech Systemic Capsids

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    Collection
    ...Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate. Image...Ready-to-use AAV available from Addgene's viral service. Engineered systemic constructs from Viviana ...As part of our Viral Vector Packaging Service , Addgene is distributing ready-to-use viral preparations...depth descriptions of each serotype, check out Addgene's blog on Systemic Capsids . To review performance...data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER Center’s repository...produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005) . Note on PHP.eB tropism and specific ... BiSSTe4 dTomato/nlsdTomato Control Fishell Chemogenetics 44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Syn Activator...

  3. Viral Prep Service for Plasmids and Pooled Libraries

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    Collection
    ...vectors, and general biosafety guidelines. Addgene Blog: Viral Vector Posts Content from Addgene and scientists... production at Addgene, ordering information, and more. Additional Resources Addgene's Data Hub Curated...Overview of Addgene's viral preparation service for plasmids and pooled libraries. Viral preparation ... Viral Vector Packaging Service Addgene offers flexible packaging options so you can easily...viral vector is produced from a cargo plasmid in Addgene’s collection. These plasmids are created by your... to do when you receive your viral vector from Addgene, with details about storage. Biosafety Guide Documentation... the Penn Vector Core have been transferred to Addgene for distribution. Look up the IDs of particular...

  4. All Antibodies

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    Collection
    ...Browse Addgene's collection of recombinant monoclonal antibodies....Antibodies Addgene distributes ready-to-use recombinant antibodies. These monoclonal antibodies undergo application-specific...application-specific validation and quality control by Addgene as well as by our trusted partner labs. On this...antibodies currently available from our catalog. Addgene values transparency and accessibility in research...reactivity. Reactivity is based on validation data from Addgene or trusted partner labs or is predicted based on... on sequence homology with the antigen. Addgene supplies a list of recommended applications based on our...immunohistochemistry, and immunocytochemistry. Addgene will continue to work with both depositing and ...

  5. Fluorescent Protein Guide

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    Collection
    ...processes with genetically encoded fluorescent biosensors. Optogenetics Guide | Optogenetics Plasmids Use...signals, cells, or groups of cells. Chemogenetics Guide | Chemogenetics Plasmids Use small molecules to activate...100 empty backbones. Additional Resources at Addgene Addgene Blog: Fluorescent Proteins Luciferase Plasmid... to find the plasmids that are available from Addgene's depositing scientists. Plasmid... Fluorescent Proteins: Plasmids and Resources Addgene's plasmid repository contains thousands of fluorescent... activate or inhibit genetically engineered cellular receptors that affect signalling pathways within ...

  6. Plasmid Collections

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    Collection
    ... Addgene Homepage Plasmid Collections Addgene Plasmid Collections...Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus Adeno-associated...Browse Addgene’s curated collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, ... proteins, optogenetics, biosensors, and more. Addgene...Collections Addgene's repository contains 164,944 plasmids . We've gathered our curated plasmid resources...Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion Immunology Mammalian RNAi Microbiology... Neuroendocrine Tumor Research Foundation Neurodegeneration Research Collection Rett Syndrome Plasmid ...

  7. Rinehart Lab Phosphoprotein Reagents

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    Collection
    ...for the incorporation of phosphoserine into your gene of interest....phosphoserine into the growing polypeptide chain to generate the new phosphorylated protein. Protein Phosphorylation...by adding this phosphorylated amino acid to the genetic code of E. coli (Park et al., 2011). Follow up ...further details in Mohler et al. (2023) and find general tips on phosphoprotein expression in rEcoli XpS...Barber et al., 2018). This phosphosite library was generated in a single mixed pool by expressing and purifying...assessments. The Mode #1 phosphosite library can be generated with either phosphoserine using SepOTSλ (Plasmid...synthesized using supD tRNA ( SupD (Plasmid #68307) ) to generate the phosphosites with serine instead of phosphoserine...

  8. Antibody Guide

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    Collection
    ...Recombinant antibodies are created by cloning antibody genes from specific hybridoma cells into plasmids. Plasmids... tubulin (sometimes referred to as housekeeping genes). This allows for relative comparison of expression...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production...with myeloma cells (immortalized B cells). The genetic reorganization that creates variety in the antibody... be produced from plasmids, with an antibody’s genetic code inserted into the plasmid. This produces the...antibody is the antigen binding site. In B cells, the genetic region responsible for the variable region goes...epitopes on one antigen. Monoclonal antibodies are generated from cultured B cells, where each culture should...

  9. DNA Service - Cloning Grade DNA

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    Collection
    ...assistance at help@addgene.org . Don’t See What You’re Looking For? At this time, Addgene has made a limited...Grade DNA Service Details Plasmids Available FAQ Addgene is offering Cloning Grade DNA (cgDNA) for over ...start cloning upon arrival of their plasmid from Addgene – reducing the time to experiments by removing ...Standard Quality Control Plasmid verified by next generation sequencing Plasmids Available as Cloning Grade...the plasmid sequence has been validated by next generation sequencing. The DNA concentration and 260/280...resources or scientific support? Please see our Addgene Protocols for resources you may find helpful. Our... Please note that this does not guarantee that Addgene will make cloning grade DNA preps of this plasmid...

  10. SARS-CoV-2 Pseudotyped Virus

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    Collection
    ...Browse Addgene's COVID-19 collection of SARS-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids...the live virus. Alternatively, it is possible to generate pseudovirus, where the structural and functional...protein and you will find examples of each in Addgene's collection: HIV-based lentiviral particles Murine...particles Vesicular stomatitis virus particles See Addgene's Viral Plasmids and Resources page for more information...lists expression plasmids that are suitable for generating pseudotyped virus for SARS-CoV-2 and other coronaviruses...virus protocol for coronaviruses (DOCX, 184 KB) Addgene Blog Posts Viral Vectors 101: Pseudotyping Antibody...Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Addgene COVID-19 Resources and Collections COVID-19 and...

  11. Antibodies

    Type
    Collection
    ...Overview of Addgene's ready-to-use monoclonal recombinant antibody collection. These affinity reagents... Antibodies Addgene distributes ready-to-use recombinant antibodies...experimental data for recommended applications. Addgene values transparency and accessibility in research...All Antibodies Ready to use antibodies? Browse Addgene’s collection of antibodies developed and validated...Production Interested in our production process? At Addgene, we provide transparency in our production process...antibody. Antibody Blog Posts Recipient Instructions Addgene Help Center Antibody Protocols Looking for Antibody...

  12. NETRF

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    Collection
    ...Addgene Depositors that have received funding from the Neuroendocrine Tumor Research Foundation...around the world. The NETRF is grateful for the generous support of philanthropic individuals and foundations...Ramesh Shivdasani Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine Cells and ...Carcinoid Tumors Qiao Zhou Harvard University Epigenetic Regulators of Intestinal Endocrine Cells and ...Bernstein Broad Institute Identifying Altered Epigenetic States and Drivers in Intestinal Carcinoid and...

  13. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...England Biolabs has deposited plasmids at Addgene to generate SNAP, CLIP, ACP or MCP tagged plasmids for...associated with this technology are available at Addgene for distribution to the research community. Key...synthases needed for use of these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (...and the ACP and SFP Synthases. Users may still generate their own fluorescent or non-fluorescent CoA substrates...

  14. Fluorescent Proteins: FRET

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    Collection
    ...Fixed Cells Additional Resources at Addgene Learn more on the Addgene Blog: Introduction to FRET and Tips...many FRET-based biosensors in Addgene's Biosensors Collection . Generate custom FRET-based biosensors ...Bioluminescence Resonance Energy Transfer (BRET) . Browse Addgene's Luciferase Plasmid Collection or the Promega Plasmid...

  15. E11 Bio PRISM Collection

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    Collection
    ... proteins under the constitutive CAG promoter (Addgene #242764–242781), but E11 Bio has also developed...of Cre-dependent, hSyn-Tet inducible plasmids (Addgene #242782–242799) suitable for targeting specific...Stochastic infection of pooled AAVs delivered in vivo generates unique combinations of protein bits. B) Multiplex...(Link opens in a new window) More Resources at Addgene: AAV Packaged on Request AAV Guide Cre-Lox Plasmid...

  16. Allen Institute for Brain Science AAV Enhancer Collection

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    Collection
    ... For more details, visit the Allen Institute's Genetic Tools Atlas (Link opens in a new window) . Figure... in adult mice. Adapted from Allen Institute's Genetic Tools Atlas (Link opens in a new window) . Allen...Striatum Pallidum Hypothalamus Cerebellum Whole brain Addgene ID Plasmid Allen Institute ID Enhancer ID FP/Recombinase...of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types. Cell , 188 (11),...

  17. Institute for Protein Innovation

    Type
    Collection
    ...secreted proteins from a collaboration between Addgene and IPI....sorting process. This combination allows IPI to generate antibodies to highly conserved proteins and other...Other Markers Antibody Collection page to browse Addgene’s full catalog of antibodies that target epitope...

  18. Trimmer Lab NeuroMab Collection

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    Collection
    ...deposited with Addgene for distribution to the research community. Protocols for the generation R-mAbs plasmids...researchers will be able to easily and inexpensively generate R-mAbs by transiently transfecting the plasmids...produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target Target Species Host Species Isotype...musculus (mouse)/R. norvegicus (rat) rat IgG2a Addgene distributes ready-to-use recombinant antibodies...application-specific validation and quality control by Addgene as well as by our trusted partner labs. Learn More...produce scFvs from transfected mammalian cells. Addgene ID Plasmid Hybridoma Target Target Species Host...produce nanobodies from transfected bacterial cells. Addgene ID Plasmid Hybridoma Target Target Species Host...

  19. Fluorescent Protein Guide: Empty Backbones

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    Collection
    ...fluorescent tags to create fusion proteins with your gene of interest....Reading and Resources Addgene Blog: Which Fluorescent Protein Should I Use? Addgene Blog: Choosing Your ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  20. Tags and Other Markers

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    Collection
    ...application-specific validation and quality control by Addgene as well as by our trusted partner labs. To learn...our Suggest an Antibody form, or e-mail help@addgene.org to help us improve our resources!...
Showing: 1121 - 1140 of 1149 results