Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 311 results for: cas9 genes

Showing: 151 - 180 of 311 results

  1. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 51306 pUC57-Simple-gRNA...designed for use in Xenopus. Cut Fully functional Cas9 enzymes designed to introduce a double-strand break...surrounding the DSB is introduced along with the Cas9 and gRNA plasmids, the cell may instead repair the...faithfully introduce specific genomic changes. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression...backbone T7 BsaI In vitro transcription none, need Cas9 plasmid Chen Do you have suggestions for other plasmids...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...

  2. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...replace” gene editing method in which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter...ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter...contains Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection...directs the Cas9 nickase to the target sequence where it nicks the non-target strand and generates a 3’ flap...nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can be used in PE2...PE6 - small prime editors with optimized RT and/or Cas9 domains PE7 - addition of RNA-binding exonuclease...

  3. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...possible deletion strategies for genes and non-coding elements. For creating a gene knockout, two sgRNA located...evaluate the RNA, perform RT-qPCR for gene expression of the relevant gene 7,8 . To evaluate the protein, perform...Article Citation Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9. Bauer DE, Canver... CRISPR/Cas9 is an inexpensive, facile, and efficient genome editing tool that allows genetic perturbation...perturbation of genes and genetic elements. Here we present a simple methodology for CRISPR design, cloning,...for efficient loss-of-function studies of genes and genetic elements in mammalian cell lines. Protocol...excerpted from: Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9. Bauer DE, Canver...

  4. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ...explains how gene drives work and what work he's planning on doing with CRISPR-based gene drives focusing...member of the Addgene Scientific Advisory Board with a particular interest in generating Cas9 based tools...Additional Resources on the Addgene Blog Learn More About Alex's Work with Cas9 Activators Michael Koeris...developing new CRISPR tools, and the potential uses of gene drives. Listen here! Podcast breakdown (Time : ...- 31:25 : Nature's defenses against gene drives. 31:26 - 33:08 : Plans for future work at the Wyss Institute... gene drives...became a member of the Addgene advisory board where he will help shape Addgene's future. Listen to this...

  5. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...multiple terminators can serve to interrupt genes (or gene clusters) in metabolic engineering. So far,...consists of one plasmid that expresses the S. pyogenes Cas9, a temperature-sensitive replicon for self-curing...pTargetF with the ccdB gene containing BsaI restriction enzyme sites at its ends to generate pEcgRNA. The ccdB...to successfully delete genes of interest in BL21(DE3) using the newly generated pEcCas/pEcgRNA system....used MUCICAT to optimize the gene dosage of glucose dehydrogenase and generated a strain that could achieve...J, Yang S (2015) Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System. Appl Environ...-curing, an arabinose inducible lambda-red gene, and an IPTG-inducible sgRNA sequence targeting the pMB1...

  6. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ...specifity of Staphylococcus aureus Cas9 (SaCas9 KKH variant) Engineered CRISPR-Cas9 nucleases with altered PAM...the Cas9 endonuclease) that cleaves the target DNA. The Joung lab recently described gRNA and Cas9 expression...for the EGFP Reporter Gene Guide RNA expression plasmids for endogenous human genes Guide RNA expression...these vectors for efficiently modifying endogenous genes in zebrafish (Hwang and Fu et al., Nat Biotechnol...expression plasmids for endogenous zebrafish genes... (AsCpf1 and LbCpf1) for use in mammalian cells SpCas9 variants with no detectable off-target effects ...

  7. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...CRISPR/Cas9 methodology with T cell receptor engineering (Ref 5)  as described by Blue Flame Addgene depositor...using randomly integrating viral vectors, CRISPR/Cas9 genome editing allows targeted engineering of the.... "Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection." Nature 543.7643 (2017...Additional Resources on the Addgene Blog Read Additional Cancer Blog Posts Addgene's Cancer Collection Pages...this multi-pronged attack. Inflammation and tumorigenesis The conference opened with a very interesting... between tumor and immune cells in real time. Addgene depositor Professor Max Krummel from UCSF showed...poster is pTomo-Ras which has been deposited at Addgene was used to produce the glioblastoma model for ...

  8. Zebrafish Plasmid Collection

    Type
    Collection
    ...zebrafish gene of interest? Search Addgene's collection for plasmids that contain a zebrafish gene or sequence...Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector System... zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart research...zebrafish-specific gene expression database that provides information about when genes are active in developing...developing embryos. zfRegeneration - A database for gene expression profiling during regeneration. Seurat - R ...-type specific conditional gene inactivation - Jeffrey Essner Lab GeneWeld Vectors for Targeted Integration...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference...

  9. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...CRISPR/Cas9 genetic circuits References: Nielsen AA, Voigt CA. Multi-input CRISPR/Cas genetic circuits...binding proteins with Cas9 guided by different promoters. The challenge in building genetic circuits is getting... to turn on genes at different times. It requires more complex synthetic networks. Addgene: What hurdles... that stop it from being used. One thing is that Cas9 is toxic. When cells carry CRISPR circuitry, it ...on an engineering project including hundreds of genes and regulation and resource control - everything...gives us enough hardware to control hundreds of genes. We’ve created enough regulatory elements, CRISPRs...the scale of genetic engineering, with the ultimate objective of genome design.” It’s genetic engineering...

  10. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: Cas9 The ...long ago, spCas9 was the only Cas enzyme widely available and applied by researchers for gene targeting...they're available through Addgene. So whether you're looking for the exact Cas9 plasmid used by Jinek et...Resources on Addgene.org CRISPR software and resources Addgene CRISPR Guide Addgene’s CRISPR 101 eBook...and trans activity of each entry. For example, SpyCas9a (one of the most frequently used Cas enzymes) ... trans activity. AsCas12a, on the other hand, generates staggered DNA nicks at its target in cis and cleaves...end goal. CasPEDIA also features useful links to Addgene resources throughout the site. In the Experimental...

  11. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...Resources on the Addgene Blog: Learn about CRISPR RNA Editing with REPAIR Get tips for using Cas9 Transcriptional...than other Cas13s and a whopping 33% smaller than Cas9! Their small size makes then easy to package in ...significant off-targets, CasRx displayed zero. Unlike Cas9, for which efficiency varies widely across guide...In this case, Konermann et al. targeted MAPT, the gene encoding dementia-associated tau, delivering dCasRx...possibilities. To identify Cas13d, both groups used very general bioinformatic screens that looked for a CRISPR ...regulating methods: small hairpin RNA interference, dCas9-mediated transcriptional inhibition (CRISPRi), and...scientific sharing will continue to grow, and we at Addgene appreciate our depositors for making their tools...

  12. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 46759 pT7-gRNA ...none, need Cas9 plasmid Chen and Wente 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid...at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Vector Type Promoter PI Publication Empty...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  13. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

    Type
    Blog Post
    ...Energy’s Joint Genome Institute for uncharacterized genes that were proximal to a CRISPR array and the universal...CRISPR-Cas systems containing cas1, cas2, cas4, and a new gene cas14. cas14 encodes for a small Cas protein (40...-Cas systems. There are 24 variants of the cas14 gene that cluster into 3 subgroups (cas14a-c). All these...primitive version of the larger and more complex Cas9 and Cas12 proteins. Cas14 cleaves ssDNA But what...potential of Cas14, the Doudna lab cloned the cas14 gene together with the adjacent CRISPR array and intergenic... and cleave a targeted sequence of ssDNA. Unlike Cas9, Cas14 does not require a PAM sequence. In addition...presence of infectious organisms (ex: SARS-CoV-2) and genetic mutations. By providing a gRNA specific of a viral...

  14. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...among other things) gene delivery, generating pluripotent stem cells, and developing gene therapies for monogenic...Cre-dependent gene expression, gene knockout, and selection marker removal. CRISPR technology pdCas9-humanized...and lentiviral vectors that are used for gene regulation, gene of interest expression, knockout, and screening...codon-optimized Cas9 under the control of Murine Stem Cell retrovirus promoter for mammalian gene knockdown....Expressing your gene of interest pLenti-puro (Plasmid #39481) You can clone your gene of interest into...inducible gene overexpression, knockdown (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion...This is a 3rd generation lentiviral backbone from the Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette...

  15. CRISPR Plasmids - Yeast

    Type
    Collection
    ... In Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 49014 pRPR1_gRNA...dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down gene expression...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Base Edit Catalytically dead dCas9 fused ...Description Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or ... plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter Selectable... plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter Selectable...

  16. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...using CRISPR/Cas9 to edit a gene, the expression of Cas9 is no longer necessary. However, Cas9 can be toxic...Gag, Pol, Rev, and Tat genes. Specifically for our knowledge here, the Pol gene encodes three separate...Insertional mutagenesis combined with acquired somatic mutations causes leukemogenesis following gene therapy...insertional mutagenesis. This integration could lead to an undesirable interruption of a critical gene. Because...Insertional mutagenesis is especially concerning in clinical settings, as evidenced by retroviral gene therapy...like creating stable cell lines, delivering CRISPR/Cas9 to cells for genome editing, and introducing inducible...transcriptase), and IN (integrase). These viral packaging genes are required for the production of lentiviral particles...

  17. CRISPR Guide

    Type
    Guide
    ... available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...dual nickases to generate a knockout or gene edit; or using Cas9 to generate large genomic deletions or ...strand SpCas9-HF1 - disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading...Plasmids: Purify Cas9 Alternatives for CRISPR Genome Engineering S. pyogenes Cas9 (SpCas9) is the most commonly...new tools. Figure 3: Overview of Cas9n Nickase and dead Cas9 The two Cas9 nuclease domains, RuvC and HNH...enhanced specificity by engineering Cas9 into high fidelity Cas9s (hfCas9) . There is currently no defined...application. Cas9 Orthologs While SpCas9 is the most commonly used Cas enzyme, additional Cas9 orthologs ...

  18. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    ...technology of the year in our collection. CRISPR/Cas9 - At Addgene, 2013 will be known as the "Year of the CRISPR...frequently requested. Ubiquitin - One particular gene we saw increased interest in from 2008 to 2009 was...expression and pLVTHM, a lentiviral plasmid for mammalian gene silencing. TALENs - Genome engineering methods improved.... So far, we have distributed over 10,000 CRISPR/Cas9 plasmids to more than 3,000 labs around the world...Addgene was founded 10 years ago today. In that time, Addgene has shipped over 350,000 individual plasmids...biosensors, optogenetics, and other imaging tools. The GCaMP5 and GCaMP6 series of genetically encoded calcium...Top 10 plasmid technologies distributed through Addgene over our first 10 years. 10 most distributed plasmid...

  19. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector...This protocol can be used to generate stable cell lines expressing a gene of interest from an integrated...A549 cells stably expressing Cas9. A549 cells were transduced with lentiCas9-Blast and then selected with... Protocols Generating Stable Cell Lines with Lentivirus Generating Stable Cell Lines with Lentivirus... may have 1 copy of the transgene, while others have >1 copy of the transgene in their genome. This can...high levels of the transgene may have reduced growth rates, especially if that transgene is toxic. Eventually...low-level transgene expressors may take over the culture. To overcome this, consider generating monoclonal...

  20. CRISPR Plasmids - Tagging

    Type
    Collection
    ...listed below. When targeting other gene loci, you will prepare the gene-specific CRISPR and donor vectors...pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific...Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which expresses an untagged Cas9 AND a gRNA...Drosophila cells that uses PCR to generate both an expression cassette for the Cas9-programming sgRNA and HR donors...multiple genes. Plasmids can be found associated with the following article: Paix, et al. Genetics 2014 Allen...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR...repair template design. How to use CRISPR to tag your gene of interest Mendenhall and Myers Tagging System ...

  21. Plan Your Experiment

    Type
    Guide
    ... RNA delivery of Cas9 and gRNA In vitro transcription reactions generate mature Cas9 mRNA and gRNA, which...used to generate transgenic embryos Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein ...knockin. Select gene and genetic element to be manipulated In order to manipulate a given gene using CRISPR...mutant allele of a gene ( point mutant )? Increase or decrease expression of a target gene? Once you have ...disrupt gene function in a particular cell type or organism without a specific preferred mutation Cas9 (or...point mutation or inserting a tag Cas9 (or Cas9 nickase); Base editor Single (or dual) gRNA targeting the...concern, Cas9-gRNA ribonucleoprotein (RNP) complexes are advantageous due to the transient Cas9 expression...

  22. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...expression of your gene of interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire...high-throughput CRISPR/Cas9 gene modification in Caenorhabditis elegans." Genetics 202.4 (2016): 1277-1288...toolkits available at Addgene.  C. elegans open resources The C. elegans Neuronal Gene Expression Map & Network...Additional resources on the Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse... The CeNGEN goal is to establish a comprehensive gene expression atlas of every C.elegans  neuron. Scientists... C. elegans embryo when all essential embryonic genes are individually depleted via RNA interference (...based bipartite expression system that can regulate gene expression in C.elegans. In this system a promoter...

  23. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...numerous applications, including transgenesis, gene-trap screens, and gene editing. How does the piggyBac...levels of your gene of interest, as needed. Controlling the expression levels of your gene may be particularly...Transposagen.   How does piggyBac™ enhance gene editing? Gene editing can now be accomplished in a more...(CRISPR/Cas9, TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely... How does the piggyBac™ gene editing system compare with other methods of gene editing? There are primarily... a variety of gene editing technologies including Zinc Fingers, TALENs, and CRISPR-Cas9 nucleases. Mathew...Decoding the Epigenetic Heterogeneity of Human Pluripotent Stem Cells with Seamless Gene Editing.,” Methods...

  24. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... Strong expression: A CMV promoter drives robust gene expression in mammalian cells. Flexible tagging:...tag. Selectable and stable: A puromycin resistance gene makes stable cell line creation a breeze. Modular...Bentley For RNA-guided genome editing, think beyond Cas9 with STITCHR: a combined CRISPR / retrotransposon.... Figure 4: STITCHR consists of a nicking Cas9 fused to a shortened R2Tocc transposon protein, ...for scarless insertion of the payload (teal). The Cas9 gRNA targeting guide and the transposon RNA homology...scientists fused the retrotransposon protein R2Tocc to a Cas9 nickase to improve target recruitment and nicking...labeling By Alyssa Shepard To fully understand how genes are regulated, it's important to know what proteins...

  25. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI ...catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes, it generates a double-strand...PI Publication Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids... CRISPR dCas9-FokI CRISPR: dCas9-FokI...with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...

  26. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Plasmid Gene/Insert Selectable Marker PI Publication Purify A catalytically inactive Cas9 (dCas9) can be...(HDR). ID Plasmid Gene/Insert Promoter Selectable Marker Publication Prime Edit Cas9 H840A nickase fused... to immunoprecipitate FLAG-tagged Cas9. In the CAPTURE system, Cas9 is tagged with a biotin acceptor site...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Visualize A catalytically inactive Cas9 (dCas9...dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down gene expression...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or ...

  27. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...Truncated gRNAs for Regulating Gene Expression and CRISPR 101: Epigenetics and Editing the Epigenome (plus...and CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs...Joining and PITCHing MMEJ as an Alternative Route for Gene Editing Day 9: Precision editing: Single Base Editing...Genome-Wide Screening Using CRISPR/Cas9 and Plasmids 101: NGS Quality Control for Pooled Libraries Day 12: New... CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional regulation:...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that...flame for its adenine base editing capabilities. Epigenetics: CRISPR has made it possible to design programmable...

  28. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...70% of human genes have a counterpart in zebrafish. Understanding the function of these genes in fish will...unknown functions for genes, and lays the groundwork for understanding the gene networks that regulate...zebrafish-specific gene expression databases that provide information about when and where genes are active in...TALENs, and more recently CRISPR/Cas9. Another popular targeted gene deactivation method in zebrafish ...investigations. Genetic resources for zebrafish A common approach to unravel the gene networks that drive...model organism is its amenability to genetic manipulation. Tools for gene manipulation together with information...Additional mutagenesis projects have since been initiated and today thousands of mutations in genes affecting...

  29. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...found in the same gene. If we only have small, directed editing abilities, any gene editing treatment ...functional copy of the entire gene, allowing a one-size-fits-all approach to gene replacement therapy for most...programmable gene insertion with PASTE. The PASTEsystem involves insertion of landing sites via Cas9-directed...insertions, leaving a several kilobase-sized gap in the gene editing field. The AbuGoot lab has now developed...to work. (It was also found to be independent of gene repair.) This lowers the chance of the insert being...activity. They now had a system that could integrate genes up to 36 kb in length, with an integration rate ...bylanding site recognition and integration of cargo via Cas9-directed integrases.   Use PASTE can be delivered...

  30. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...lab utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate gene targets. To accomplish...fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids... plasmids dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion...CRISPR Collection The Brzezinski lab investigates gene regulation in the context of the developing mouse...taken advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several...pX458 plasmid to expand its usefulness for the lab's gene regulation and developmental studies. Key plasmid...fate specification, yet differs from deleting both genes . Development. doi: 10.1242/dev.190272. PMID: 32541005...
Showing: 151 - 180 of 311 results