We narrowed to 402 results for: CAPS;
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TypeProtocol...digital cameras attached to them that allow you to capture images directly to a computer, but many microscopes... your drawing skills. Regardless of your image capture method, be sure to record the magnification used...
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AAV Purification by Iodixanol Gradient Ultracentrifugation
TypeProtocol...contaminants with lower densities, including empty capsids. The 60% step serves as a cushion for genome-containing... number of contaminants in each fraction. * AAV capsid proteins VP1, VP2, VP3; M protein marker. Image... -
What is Polymerase Chain Reaction (PCR)
TypeProtocol...you are using a polymerase with proofreading capabilities. Note: See manufacturer’s instructions for specific... of the reaction mix. Its solvent and buffer capabilities provide a suitable environment for the DNA amplification... -
Western Blot
TypeProtocol...Microcentrifuge tubes BCA Assay, Thermo Fisher 23227 β-mercaptoethanol 4X protein loading buffer Precast SDS-PAGE ...sample for loading as follows: Add 10% v/v β-mercaptoethanol to the 4X protein loading buffer. Dilute 4X... -
Viral Vectors 101 eBook 2nd Edition
TypeBlog Post...elements; resources on different types of vectors, capsids, and tools; and helpful information on everything... -
Meet Aavery, Blugene's New Virus Friend!
TypeBlog Post... We love the way the names Aavery, Addy, and Cappy play on viral vector terms. And, the name Kendall... -
The 12 Days of CRISPR: 2021
TypeBlog Post...CRISPR, Addgene gave to me: Selective Endogenous eNcapsidation for cellular Delivery (SEND). On the fourth ... -
Addgene's 20th Anniversary Party
TypeBlog Post...photos and slideshows shared some of the moments we captured over the years, including an album of Addgenie... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog Post...: Amos BairochViral Vectors 101: Systemic CapsidsResearchers: Cynthia Arokiaraj; Viviana Gradinaru; Tim... -
New Educational Resource: The Addgene Videos Page
TypeBlog Post...How-to videos Our How-To videos feature screen-capture footage to help you navigate Addgene’s vast online... -
Summer Fun at Addgene!
TypeBlog Post...haven’t seen our webinar “Navigating an Expanding Landscape for Engineered Systemic AAVs” with the director... -
Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6
TypeBlog Post...therapeutics. After a brief stint at the venture capital firm, Flagship Ventures, Koeris helped change Novophage's... -
2022 and 2023: Reflections and Looking Forward
TypeBlog Post...and a compelling future to plan in the changing landscape of science. We are therefore embarking on a strategic... -
A Conference By Postdocs For Postdocs: Future of Research
TypeBlog Post...are not exposed to the vast non-academia career landscape and there is insufficient (or no) emphasis on ... -
Quick, Versatile Plant Transgenesis with GreenGate Plasmids
TypeBlog Post...Precision Cloning Method with High Throughput Capability." PLoS ONE. 3(11): e3647 (5 November 2008). ... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog Post...the phage field, subscribe to their newsletter, Capsid and Tail. Additional resources on the Addgene ... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post...nm, photoconverted with 405 nm light, and then captured in the red state after excitation at 561 nm. This... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog Post... for what we have accomplished. Starting with a recap of accomplishments, from learning new techniques... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog Post... mutation [3]. In the CRISPR era, the genomic landscape may be surveyed in unprecedented fashion. Targeted... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog Post... to their genes of interest, allowing for easy capture and detection of proteins and complexes, without... -
Addgene is Expanding Our Viral Vector Service!
TypeBlog Post...AAV1, AAV2, AAV5, AAV8, or AAV Retrograde (AAVrg) capsids. Please be aware that this ordering process will... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post..., 10 μm (HEK293T), 20 μm (neurons). Figure and caption adapted from Kagiampaki et al., 2023. nLight... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog Post..., smaller binary vectors with a larger cloning capacity would be very useful: enter pSIM24! To build a... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post... tools should further enhance genome editing capabilities in Drosophila. References: Multiple new site-specific... -
Future of Research Conference - Remarkable Opening Session
TypeBlog Post...Polka said as she started things off, “We are all capable of contributing to change.” Check out Joanne's... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog Post...this yeast cloning kit) she intends to make yeast capable of desaturating fatty acids. Identification of ... -
What's the Best Way to Elute and Store Your Plasmid DNA?
TypeBlog Post.... The two solutions may differ though in their capacity to maintain DNA stability during storage over ... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog Post...enzymes have been engineered to have many unique capabilities which has advanced what type of edits can be... -
Embracing Serendipity: A Crucial Element in the PhD Journey
TypeBlog Post... times (or use the notes app on your phone) to capture fleeting thoughts and connections that emerge during... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog Post...with master's have Job descriptions often fail to capture all of the responsibilities of a role, so it helps... -
10 Ways to Share Your Science!
TypeBlog Post...available yet, order a copy of Nancy Baron’s book “Escape from the Ivory Tower.” 9) If you think you might... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...improved tissue penetrance and live cell imaging capabilities. Upon developing a new imaging-based bacterial... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog Post... answer questions and provide support. Growing capacity - ABRC outreach today Over the years, the outreach... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post...successfully reprogram. Unfortunately scRNA-seq only captures the gene expression of cells at a single time ... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post... exciting to test this approach with different capsids. Most importantly, because our AAV yields are improved... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog Post...not get packaged in the AAV vector. The cloning capacity between the ITRs is ~4.4 kb. ITRs are inherently... -
Meet the New Addgene Board Members!
TypeBlog Post... experience from the scientific community gets captured,” he says. “It’s not just a reagent, it’s an annotated... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog Post...experimental image result that you generated with a caption. For microscopy images, please include channel ... -
Summer SciComm: Science Storytelling
TypeBlog Post...’t need to be anything fancy; it just needs to capture your audience’s attention enough to make them decide... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog Post...Together, we can transform the antibody sharing landscape as well. References Andrews NP, Boeckman ...