We narrowed to 727 results for: ACE;

...each DNA target have high sequence identity to the spacer sequence and contain a PAM sequence just downstream...

...Monitor small biomolecules or other physiological intracellular processes with genetically encoded fluorescent...

...structures of proteins of medical relevance, and place them in the public domain without restriction. Many...

...single plasmid, multiple orders would need to be placed through our website. Every order will need to be...

...Key plasmid features and pX458 modifications: replacement of the Cbh promoter with EF1alpha addition of...

...inhibitory. PSAMs are engineered α7 nicotinic acetylcholine receptor (nAChR) domains that respond specifically...

...active site", "active site loop", or "subunit interface") ID Plasmid Description Gene/Insert Mutations...

...recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration between...

...coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256 Expresses...

...FokI nuclease operates as a dimer, cutting in the spacer region between two distinct ZF target sites. (B...

...Cells AAV Purification by Iodixanol Gradient Ultracentrifugation AAV Titration by ddPCR AAV Titration by qPCR...

... directly to the brain parenchyma or subretinal space Serotypes The following serotypes were developed...

...the Voytas Golden Gate TALEN Kit and are used in place of pTAL1, 2, 3, or 4. For both plasmids sequence...

...Community (Link opens in a new window) Research workspace on Protocols.io (Link opens in a new window) ....

...-C1* (V70) (Plasmid #188537) a better choice in place of the popular SepOTSλ (Plasmid #68292) . An important...

...Iodixanol Gradient Ultracentrifugation AAV Purification by Iodixanol Gradient Ultracentrifugation You may also...tube at the interface of the 60% and 40% gradient (see Figure 2) with an 18 ga needle. Place the first ...fractions per tube. Avoid the proteinaceous material near the 40–25% interface. Pro-Tip As soon as the top...care to avoid collecting the proteinaceous material at the 40–25% interface. Repeat for each QuickSeal ...Iodixanol gradient after ultracentrifugation. The arrow indicates the 60–40% interface. The vertical black ...Follow this protocol using Iodixanol gradient ultracentrifugation to get pure adeno-associated virus (AAV) ... be completed in one (long) day. Equipment Ultracentrifuge T70i rotor or equivalent Reagents Opti-Prep...

...- Cas9 Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a microbial ...

...using various delivery methods including intracerebroventricular (ICV) delivery in newborns, and retro-orbital...

...Brd3R from five separate lentiviral plasmids The acetyllysine reader BRD3R promotes human nuclear reprogramming...

...Edit References CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a powerful system...