...Calcium Sensors HaloCaMP1a HaloCaMP1b NIR-GECO Acetylcholine Sensors iAChSnFR GRAB_ACh Dopamine Sensors dLight... Promoter Sensor Label Activity Serotype PI Acetylcholine Sensors 137950 pAAV.hSynap.iAChSnFR Syn iAChSnFR...

...or repression. The TCS light sensors described in ACS Synthetic Biology (2014) represent the newest versions...Photobiology: Optogenetics for E. coli. Schmidl, et al. ACS Synth Biol. 2014 Nov 21;3(11):820-31. Inncredible...compartment (e.g. the plasma membrane, the extracellular space, or the lysosomes) can take many paths. Understanding...that is quick, versatile, and robust. Dubbed the LACE system for light-activated CRISPR-Cas9 effector,...the CRISPR system. The ease and flexibility of the LACE technology makes this system widely accessible for...thereby creating a single-molecule reporter of intracellular processes. Multimerization via the SunTag system...combination of all 11 att pairs in series with unique spacer sequences (pMemoryArray) gives a DNA sequence capable...

...Cre fragments (NCre and CCre, respectively) and placed under the control of different promoters. Expression...CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular vesicle targeting sequence and CFP UBC Mammalian...

...change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible...

...Mitogen-activated protein kinases (MAPKs), also known as extracellular signal-regulated kinases (ERKs), are serine/...

...each DNA target have high sequence identity to the spacer sequence and contain a PAM sequence just downstream...

...Monitor small biomolecules or other physiological intracellular processes with genetically encoded fluorescent...

...structures of proteins of medical relevance, and place them in the public domain without restriction. Many...

...scientific community that has established a common place for deposited plasmids. ASAP Discovery Consortium...

...inhibitory. PSAMs are engineered α7 nicotinic acetylcholine receptor (nAChR) domains that respond specifically...

...Key plasmid features and pX458 modifications: replacement of the Cbh promoter with EF1alpha addition of...

...single plasmid, multiple orders would need to be placed through our website. Every order will need to be...

...active site", "active site loop", or "subunit interface") ID Plasmid Description Gene/Insert Mutations...

...base editors convert adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite...

...What are some current obstacles that researchers face right now (e.g., not enough protocols, cost, not...

...Iodixanol Gradient Ultracentrifugation AAV Purification by Iodixanol Gradient Ultracentrifugation You may also...tube at the interface of the 60% and 40% gradient (see Figure 2) with an 18 ga needle. Place the first ...fractions per tube. Avoid the proteinaceous material near the 40–25% interface. Pro-Tip As soon as the top...care to avoid collecting the proteinaceous material at the 40–25% interface. Repeat for each QuickSeal ...Iodixanol gradient after ultracentrifugation. The arrow indicates the 60–40% interface. The vertical black ...Follow this protocol using Iodixanol gradient ultracentrifugation to get pure adeno-associated virus (AAV) ... be completed in one (long) day. Equipment Ultracentrifuge T70i rotor or equivalent Reagents Opti-Prep...

...coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256 Expresses...

...FokI nuclease operates as a dimer, cutting in the spacer region between two distinct ZF target sites. (B...

...base editors convert adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite...

... directly to the brain parenchyma or subretinal space Serotypes The following serotypes were developed...