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We narrowed to 965 results for: Abo

Showing: 101 - 120 of 965 results

  1. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...cool down to about 50 °C (about when you can comfortably keep your hand on the flask), about 5 mins. Optional...concentration of approximately 0.2-0.5 μg/mL (usually about 2-3 μl of lab stock solution per 100 mL gel). EtBr... of the tip of the pipette into the buffer just above the well. Very slowly and steadily, push the sample... of the way down the gel. A typical run time is about 1-1.5 hours, depending on the gel concentration ...

  2. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...personal protective equipment including laboratory coat, laboratory goggles, and gloves. Sodium azide containing... not already been done. We typically start with about 250 mL of supernatant and add 250 µL of 100X protease... sample among multiple columns. For information about the total binding capacity for your columns, refer...setting. If the concentration of the pooled sample is above 1.0 mg/mL proceed to Option 1 with a buffer exchange...based on the concentration of the pooled sample above. Option 1: Buffer exchange using a desalting column...

  3. Video Library

    Type
    Protocol
    ... Protocols Video protocol guides for standard laboratory procedures. How-To Videos Screencapture walk-...Electrophoresis Gel electrophoresis is the standard laboratory procedure for separating DNA by size for visualization... MTA FAQs Answers to frequently asked questions about the MTA process Help Center How to Deposit Your ...projects in the works, and all of the things she loves about working at Addgene! Maria Soriano Maria Soriano,...in science, the importance of international collaboration, and offers career advice for scientists looking...

  4. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...and Two (BSL-1 and BSL-2) Safety measures for laboratories operating at BSL-1 and BSL-2 Watch the Video...maintenance and use Watch the Video! Pipetting Learn about selecting the correct pipettor and pipette tip, ...the pipette Watch the Video! Centrifugation Learn about selecting and using a centrifuge to separate different... a liquid sample Using a Light Microscope Learn about the parts of a light microscope and its use Weighing...Weighing Reagents Learn how to weigh laboratory materials on a balance Basic Molecular Biology Name Description...

  5. Centrifugation

    Type
    Protocol
    ...Learn about selecting and using a centrifuge to separate different components in a liquid sample....Introduction A centrifuge is a common piece of laboratory equipment used to separate different components.... Floor Centrifuges sit on the floor and can be about bench height. They often spin at similar speeds ...tabletop microcentrifuge like the one in the picture above. Wear PPE appropriate for the lab space in which...

  6. AAV Production in HEK293 Cells

    Type
    Protocol
    ....8-fold. See the recipe for D1 + 0.1 M sorbitol above. Carefully pour off the media into a waste container...in a total of 5 mL of cell lysis buffer (recipe above). Pipet back and forth to resuspend each pellet ...pellets and keep on ice. Process the cell pellet from above as follows: Resuspend and lyse the cells by adding...tube containing the resuspended virus from step 12 above. Add 50 units of benzonase per mL of viral suspension...

  7. Weighing Reagents Protocol

    Type
    Protocol
    ...balance in the photo above has a capacity of 200 g, as indicated by the number above the "Zero" button. ... reagents for a stock mixture is an essential laboratory technique, as imprecise measurements can affect...

  8. Immunocytochemistry

    Type
    Protocol
    ...water Microscope slide Anti-fade mounting medium Laboratory wipes 15 mL conical tubes 50 mL conical tubes...paraformaldehyde and follow your institution's laboratory safety guidelines for disposing of waste in the...remove the coverslip. Blot the coverslip with a laboratory wipe to remove excess liquid. Add 1 drop of anti-fade...

  9. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Addgene’s Mammalian RNAi Tools page. Several other laboratories have deposited pLKO derived vectors that may...produce the same phenotype will alleviate concerns about off-target effects. B.2 Ordering Oligos Compatible...should be decontaminated with at least 10% bleach. Laboratory materials that come in contact with viral particles...work in a BL2 facility. If you have any questions about what safety practice to follow, please contact your...

  10. Protocol - How to Design Primers

    Type
    Protocol
    ...amplification process. When designing, if unsure about what nucleotide to put at a certain position within...capabilities. Taking into consideration the information above, primers should generally have the following properties...

  11. Protocol - How to Streak a Plate

    Type
    Protocol
    ...resistance and your initials. Labeling within a laboratory setting is important for organization, and it...a section of the plate, as shown in the diagram above, to create streak #1. Pro-Tips Hold your tooth pick...

  12. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...coli after Over-Agar Plating of Carbenicillin. The above graph displays the stock concentration of Carbenicillin...concentrations that will work for this assay, and the above result represents a single experiment. For publishable...

  13. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...Addgene lists the primers used to obtain each result above the posted sequence in the "View Sequence" link....sequence than expected and wish to contact Addgene about the accuracy of your plasmid, please email help@...

  14. Water Bath Protocol

    Type
    Protocol
    ... lab! Introduction A water bath is a piece of laboratory equipment that helps bring your materials to ...even if you are using disinfectants as described above in step 3. You will also need to maintain the appropriate...

  15. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...verification, such as DNA sequencing . In the example above, digestion with enzyme RE1 will linearize the 6200bp...that you get double confirmation. In the example above, digestion with either RE3 or RE4 will give a very...

  16. Using a Light Microscope Protocol

    Type
    Protocol
    ...research and are found in many different types of laboratories. These tools allow you to observe specimens ... in the future. Conclusion Like any important laboratory instrument, you should be sure to take care of...

  18. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...approximately 50–60% confluent. For 293T cells this is about 2 × 10 6 cells in a 10 cm dish. Each 10 cm dish ...cell solution into the conditioned medium prepared above to make a new cell solution at a concentration of...

  19. Kit Free RNA Extraction

    Type
    Protocol
    ... working with the volatile reagents in the list above. Procedure Option #1 - Solution D Protocol Before...sure to prepare solution D (see reagent section above for the recipe). If using TRIzol®, jump down to ...

  20. Lentivirus ddPCR Titration

    Type
    Protocol
    ...the titer. Calculations In the experimental setup above, the following virus dilutions were used to transduce...v = Virus volume, mL *In the experimental setup above, the cells per well is 300,000 and the virus volume...
Showing: 101 - 120 of 965 results