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We narrowed to 889 results for: Cel

Showing: 101 - 120 of 889 results

  1. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Drosophila cells. N terminal tagging in Drosophila cells 3.2 MB C terminal tagging in Drosophila cells 3.3 MB...following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System The Förstemann... Allen Institute for Cell Science Plasmid Collection The Allen Institute for Cell Science has produced...tagged, human, induced pluripotent stem cell (hiPSC) lines. These cell lines were created using CRISPR and...tagging transcription factors with FLAG in human cell lines. To repeat the tagging in your system, use... from the Doyon Lab’s publication: Dalvai et al. Cell Rep. 2015 . Alternatively, cDNAs can be cloned directly... CRISPR tagging technique for use in Drosophila cells that uses PCR to generate both an expression cassette...

  2. Zhang Lab CRISPR Page

    Type
    Collection
    ...genome editing in mammalian cells (Cong et al ., Science 2013, Ran et al ., Cell 2013, Ran et al ., Nature...Matoba S, Zhang Y, Zhang F. Cell . 2013 Sep 12;154(6):1380-9. doi: 10.1016/j.cell.2013.08.021. Epub 2013 Aug...Feng G, Sharp PA, Zhang F. Cell . 2014 Oct 9;159(2):440-55. doi: 10.1016/j.cell.2014.09.014. Epub 2014 Sep...CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease...cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return to top AAV - Cas9 mouse CRISPR-Cas9...constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice the CRISPR-Cas9 system can ...recombination and fluorescence assisted sorting of cells and nuclei in addition to sgRNA expression. This...

  3. COVID-19 Resources

    Type
    Collection
    ... of the viral spike (S) protein to cellular receptors. The cellular receptor for SARS-CoV-2 and SARS-CoV...SARS-CoV-2 . TMPRSS2 - Hoffmann M, et al, 2020, Cell SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is...key step is the priming of the S protein by host cell proteases. The S protein of SARS-CoV-2 is primed...Human Angiotensin Converting Enzyme 2) is the host cell receptor mediating the entry of SARS-CoV and SARS-CoV...2 S protein and is involved in virus entry into cells. ( 2 ) FURIN - an enzyme that cleaves precursor ...2 S protein and is involved in virus entry into cells. ( 4 ) Cathepsin L - a cysteine endosomal protease...increasing viral infectivity.( 6 ) PCP4 - (Purkinje cell protein 4) is involved in beating of airway cilia...

  4. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...vectors designed to express nucleases in different cell types. Zinc finger arrays can be transferred in-...vectors designed to express nucleases in different cell types. Zinc finger arrays created using ZFC reagents...nuclease domains: pST1374 (for expression in mammalian cells or zebrafish) pDW1775 (for expression in plants)... Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish (can be used as a substitute for pMLM335... Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish (can be used as a substitute for pMLM336... Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMLM802 (for expression of a ZFN ... Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMG10 (a '“2-in-1” ZFN cassette for...

  5. Tetracycline Inducible Expression

    Type
    Collection
    ...Plasmids Collection Cre-lox Plasmids Collection Stem Cell Research Plasmids Collection Tetracycline Off Tetracycline...expression of the transactivator proteins in mammalian cells or other species by codon optimization of their ...gene of interest from two separate vectors. Stable cell lines or transgenic animals can be made to achieve... and ensures that all transfected or transduced cells express both components in the same relative ratio...derivatives can be found in Krueger et al., 2004. Some cell culture sera may contain tetracycline or its derivatives...expression of mouse Oct4, Sox2, Klf4, and Myc for iPS cell generation Rudolf Jaenisch 51543 FUW-tetO-hOKMS ...expression of human Oct4, Sox2, Klf4, and Myc for iPS cell generation Tarjei Mikkelsen 172115 PB-TO-hNGN2 Piggybac...

  6. Promega Plasmid Collection

    Type
    Collection
    ...NanoLuc fusions are useful for both intracellular and extracellular applications where you want minimal...expression systems including E. coli , mammalian cells, and cell-free systems. HiBiT Fusions HiBiT is a small...scientists and designed to streamline molecular and cellular biology experimental workflows. The collection...proteins at endogenous levels in lysates or live-cells, thus preserving physiological relevance. NanoBiT... to either LgBiT or SmBiT and expressed in live cells. When the two proteins interact, the subunits come...designed to measure molecular proximity in living cells. NanoBRET PPI assays use NanoLuc Luciferase as a...NanoBRET 618 fluorophore, as the energy acceptor. Live-cell reagents allow you to detect PPI induction and inhibition...

  7. Caltech Systemic Capsids

    Type
    Collection
    ...then cell type. For CNS: For infant rhesus macaques: Broad tropism - AAV.MaCPNS1, AAV.MaCPNS2 Cell type-biased...tropism - AAV.CAP-B22 Cell type-biased: Neurons - AAV.CAP-B10 Brain vascular cells - AAV.CAP-Mac For rats...tropism - AAV-PHP.eB Cell type-biased: Neurons - AAV.CAP-Mac Brain vascular cells - AAV9-X1.1, AAV1-X1...including: the delivery method, mouse strain, target cell type or tissue of interest, and model organism under...serotype has been reported to occur by using the cellular receptor LY6A ( Huang et al., 2019 (Link opens... above helps you choose the best capsid for your cell type. First, do you want to target CNS ...Broad tropism - AAV-PHP.eB, AAV-PHP.AX, AAV-PHP.eC Cell type-biased: Astrocytes - AAV-PHP.AX with GRE or...

  8. Ras Pathway

    Type
    Collection
    ...molecular switches that transmit signals within the cell. GTPases have an active (GTP) and inactive (GDP)...prominent roles in signaling pathways regulating cell migration, survival, adhesion, growth, and differentiation...Cancer Impact Ras is ubiquitously expressed in all cells and all tissues. Ras can activate a variety of ...pathways, which ultimately turn on genes involved in cell growth, differentiation and survival. Ras is essential...essential for regulating the growth of the cell, however when mutations occur in the Ras gene it can be... active form, constantly activating pathways for cell growth and potentially leading to cancer. Constitutively...E2F2 E2F3 E2F transcription factor ECT2 Epithelial cell transforming 2 EGFR Epidermal growth factor receptor...

  9. Luciferase Plasmid Collection

    Type
    Collection
    ...Expression of firefly luciferase from a cell-free mammalian cell lysate Marcel Bruchez 108542 pLenti-EF1a-Luciferase-IRES-Blast-WPRE...not secreted, so cells must be lysed or the substrate must be delivered intracellularly to measure luminescence...expression of luciferase in the cytoplasm of mammalian cells Peter Cockerill 212936 pGL3 Basic Vector Firefly... viral infection efficiency, to study changes in cell physiology, or as a reference level of expression...Expression of Gaussia luciferase; GFP is expressed if cells are infected with virus Christopher Buck 98580 pLX304...membrane-anchored version useful for labeling extracellular vesicles. LumiScarlet : A fusion of LumiLuc ...serve as sensors of proteome stress. pHLuc : An extracellular pH sensor consisting of a pH-sensitive GFP (...

  10. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...experiment, target cells are treated with the pooled library to create a population of mutant cells that are then...quantities of cells and therefore, may not be suitable for use when the number of cells is limited (e....ribosomal, cell cycle) 51043 — 51048 Knockout Human Sabatini and Lander 3rd 10 Varies FNLCR CRISPRa Cell Surface...e.g. primary cells). Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 to target... target cells. Therefore, you must be equipped and authorized to use lentivirus in your lab. Once you ...organism, and the library is only compatible with cells derived from that organism. All of our current pooled...140219 Knockout Human J. Chen 3rd 10 4,530 Murine Cell Surface Protein Knockout (McspKO) Library 200445...

  11. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...proliferating cells. Expression may cause repetitive recombination leading to genomic instability and cell death...and control the expression of specific genes in cells or organisms. Each recombinase enzyme recognizes... the enzyme at specific times or locations using cell-specific promoters or inducible systems, you can...promoters. Expression of both fragments in the same cell reconstitutes a functional protein, allowing recombination...expression. For example, iCre or FLPo in mammalian cells may facilitate higher rates of Cre-driven recombination...Flp-IN: A single FRT site within the genome of a host cell line serves as the target location for integration...controls such as Cre-only lines to account for cellular toxicity in your system. Using inducible Cre systems...

  12. Deisseroth INTRSECT Collection

    Type
    Collection
    ...well-defined cellular sub-populations has generally been limited to single-component cellular definitions...to enable three-feature cell targeting. C) Example of viral targeting of cells expressing Cre AND Flp,...parameters. Figure 1: Examples of intersectional cell population definitions using recombinases. A) Cre...adeno-associated virus (AAV)-borne payloads to be expressed in cells based on a doubly-specified combination of genetic...multiple recombinase-expression strategies to define cellular sub-populations of interest, including dual-transgenic...Neve R, Huang ZJ, Deisseroth K. 2014. Targeting cells with single vectors using multiple-feature Boolean...Cortex Reveals Local and Long-Range Circuit Motifs. Cell Rep. 28(13):3450-3461. PubMed (Link opens in a new...

  13. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... Anti-Pan-Neurofascin (extracellular) [A12/18R] Pan-Neurofascin (extracellular) Rat Mouse IgG2a 177439...Anti-Kv1.4 K+ channel (extracellular) [L71/5R] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 177489...Mouse IgG2a 177564 Anti-ADAM22 (extracellular) [N57/2R] ADAM22 (extracellular) Mouse Mouse IgG2a 177565 Anti-GluN2B...Anti-Kv1.4 K+ channel (extracellular) [L71/22] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 190292...neo-epitope Human Mouse IgG2a 206584 Anti-Celf4/Brunol4 [N446/80R] Celf4/Brunol4 Human Mouse IgG2a 206585 Anti-Kv11.3...-Neurofascin (extracellular) scFv [A12/18] A12/18 scFv 2t Pan-Neurofascin (extracellular) Rat Mouse 190486...Kv1.4 K+ channel (extracellular) scFv [L71/5] L71/5 scFv Kv1.4 K+ channel (extracellular) Rat Mouse 190518...

  14. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...adipose tissue-derived mesenchymal stem cells into pluripotent stem cells by a mutant adeno-associated viral...serotype also demonstrates enhanced transduction of cells in culture for ex-vivo conditioning. The AAV2(trpYF...displays enhanced transduction of hematopoietic stem cells and microglia. The AAV6(dbY-F+T-V) serotype was ...AAV-mediated transduction and targeting of retinal bipolar cells with improved mGluR6 promoters in rodents and primates...human gene therapy of glaucoma and their reversed cell entry characteristics. Gene Ther . 2020 Apr;27(3...delivery of a novel AAV vector targets ON bipolar cells and restores visual function in a mouse model of... of Primary Human Hematopoietic Stem/Progenitor Cells by AAV6 Vectors: Strategies for Overcoming Donor-Variation...

  15. mTOR Pathway

    Type
    Collection
    ...Laplante M, Sabatini DM. Cell. 2012 Apr 13;149(2):274-93. doi: 10.1016/j.cell.2012.03.017. PubMed PMID... (mTOR) is a key metabolic regulator controlling cell growth and proliferation. mTOR, a serine/threonine...signals to determine the nutritional state of the cell and regulates the switch from anabolic to catabolic...growth factors, including insulin. mTORC2 influences cell survival and cytoskeletal organization. Cancer Impact... promotes the conditions needed for uncontrolled cell growth and proliferation, including an abundant ...appears to signal upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids... one drug, many effects. Li J, Kim SG, Blenis J. Cell Metab. 2014 Mar 4;19(3):373-9. doi: 10.1016/j.cmet...

  16. Bacterial Expression Systems

    Type
    Collection
    ... location of your protein of interest within the cell, you will need to tag it with something that makes...fused to FPs and monitor their localization in live cells. ID Plasmid Tags PI Additional Addgene Protein Visualization... and expression species. The New England Biolabs Cell-Imaging Plasmid Collection contains plasmids for... inside living or fixed bacterial and mammalian cells. Addgene Blog A Guide to Selecting Fluorescent Dyes... Siouxsie Wiles 106476 pET28a_T5-ARG1 Bacterial cells Ultrasound (acoustic reporter gene, ARG1) Escherichia...Mikhail Shapiro 106473 pET28a_T7-ARG1 Bacterial cells Ultrasound (acoustic reporter gene, ARG1) Escherichia...Mikhail Shapiro 106474 pET28a_T7-ARG2 Bacterial cells Ultrasound (acoustic reporter gene, ARG2) Escherichia...

  17. iPSC Neurodegenerative Disease Initiative Plasmid Collection

    Type
    Collection
    ...nucleotide variant lines in an isogenic cellular background. All cell lines and phenotypic datasets that result...CRISPR Guide CRISPR Protein Tagging Plasmids for Stem Cell Research Neurodegeneration Research Collection The...Plasmids The following plasmids can be used to create cell lines with endogenously-tagged gene variants or ...or to differentiate human iPSCs into other cell types, as noted in the purpose field: ID Plasmid Description...neurodegenerative diseases with genomic engineering: A new stem cell initiative from the NIH. Neuron . 2021 Apr 7;109...

  18. Fluorescent Protein Guide

    Type
    Collection
    ...genetically engineered cellular receptors that affect signalling pathways within cells. Subcellular Localization...measure, and control molecular signals, cells, or groups of cells with either actuators or sensors. Chemogenetics...Monitor small biomolecules or other physiological intracellular processes with genetically encoded fluorescent...

  19. Retrovirus Plasmids

    Type
    Collection
    ...reverse transcriptase and integrase. Once in a target cell, the virus uses the reverse transcriptase in tow...γ-retroviruses are that they can only transduce dividing cells (this is because they are only able to enter the...of Large T antigen for creation of immortalized cells (see Weinberg Lab page for resistance variants) ...Expression of hTERT for creation of immortalized cells; additional plasmids include Puro resistance and...inducible expression of GFP T2A Cre fusion in mammalian cells Foijer 47916 pRXTN CMV/MSV pRXTN is a modified version...MoMSV Stable expression of luciferase in mammalian cells Wong Return to Top Do you have suggestions for other...

  20. p53 Pathway

    Type
    Collection
    ...p53 are in promoting cell cycle arrest, apoptosis, or senescence in damaged cells. The p53 name arose ...transcription factor and tumor suppressor activated by cellular stress. Cancer...transcription factor and tumor suppressor activated by cellular stresses like DNA damage, oxidative stress, and...or E2 Cyclin G CCNG1 CCNG2 Cyclin G1 or G2 CDC25 Cell division cycle 25C Cdk4/6 CDK4 CDK6 Cyclin-dependent...member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor Gadd45 GADD45A GADD45B GADD45G...
Showing: 101 - 120 of 889 results