... found to match all requested criteria. Please consider using fewer filtering options. *AAVrg = retrograde...

...OPEN OPEN aAGCTTCCTCttcattgGCTGACTGCa ldr (low density lipoprotein receptor) OZ539 and OZ540 OPEN OPEN...

...lab (main issues/factors that researchers should consider when planning experiments). What resources might...

...useful for selection or fluorescent gating of transiently transfected cells. Co-expression of both plasmids...

...E3 ubiquitin protein ligase PTEN Phosphatase and tensin homolog PUMA BCL2 binding component 3 Reprimo TP53...

...Neurodegenerative Disorders - Nicholas Cole Lab. A kit consisting of plasmids with four promoters, six fluorophores...

... found to match all requested criteria. Please consider using fewer filtering options. Browse all Control...

...non-catalytic subunit gamma PTEN Phosphatase and tensin homolog RAC RAC1 RAC2 RAC3 Ras-related C3 botulinum...

... ). The phosphorylation sites (“phosphosites”) consist of a central phosphoserine site flanked by 15 amino...

... found to match all requested criteria. Please consider using fewer filtering options. Browse all Optogenetics...

... found to match all requested criteria. Please consider using fewer filtering options. Browse all Retrograde...

...do you want to target CNS or PNS? Then consider species, and then cell type. For CNS: For infant...

... found to match all requested criteria. Please consider using fewer filtering options. *AAVrg = retrograde...

... Keep in mind that additional factors must be considered before using a validated gRNA sequence in your...

...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...

...Nestler 68552 HRE:GFP-pGK:Cre Cre and hypoxia-responsive GFP reporter HRE Lentiviral Jacks 69570 pAAV-...

...Figure 1. The two arms of the Y structure are responsible for binding the antigen, and are called the antigen...isotype of the antibody. B cells, the immune cell responsible for producing antibodies, can change the constant...binding site. In B cells, the genetic region responsible for the variable region goes through a randomized...valency of 2 IgE Protects against parasites and is responsible for driving allergic reactions such as anaphylactic...requirements, monoclonal antibodies are typically more expensive than polyclonal antibodies. Environmental factors... Minibodies are engineered antibody fragments consisting of two scFvs bound to a CH3 domain. Fab fragments... protein. Fluorophores Fluorophores are light-sensitive molecules which emit a specific wavelength of ...

...apply to using CRISPR in other organisms. First, consider the genetic manipulation that is necessary to ...Manipulation Application Cas9 gRNA Additional Considerations Knockout Permanently disrupt gene function ...ribonucleoprotein (RNP) complexes are advantageous due to the transient Cas9 expression. The table below summarizes the...details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or gRNA in a ...limit (only compatible with smaller Cas enzymes) Transient or stable expression of SaCas9 and/or gRNA Infects...cells through microinjection or electroporation Transient expression of CRISPR components Expression decreases...which is delivered to cells using cationic lipids Transient expression of CRISPR components Short window of...

...and should be considered on a case by case basis. Biosafety should always be considered with respect to...planning your experiment, the important component to consider and optimize is the transfer plasmid. 2nd generation... tat. Third generation lentiviral systems are considered safer than second generation systems, but may...) lentiviral transfer plasmids can be used in transient transfections to achieve expression of the transgene...transfer plasmids are not designed specifically for transient transfections. Therefore, there may be limited... interest using PCR. The process of subcloning consists of digesting the insert of interest from its parent...infection. In this regard, 3rd generation systems are considered safer than 2nd generation systems because the...

... the following enhancements: Increased light sensitivity Example: ArchT Increased photocurrent amplitude... Volvox carteri 570 Chronos High-speed, light-sensitive channel from Stigeoclonium helveticum 500 Chrimson...from Halorubrum strain TP009. Improved light sensitivity over Arch. 566 eArchT 3.0 ArchT with addition... delivery system. Here are some key factors to consider: Optogenetic excitation or optogenetic inhibition...neural populations at distinct times. Temporal considerations. Temporal precision is key in optogenetic experiments...Rhodopseudomonas palustris bacteria. Key feature is the sensitivity to 740- to 780-nm near-infrared light. 740–780...488 LOV domains LOV-based Light-oxygen-voltage-sensing (LOV) domains are protein sensors used by many ...