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...generate these lines have been deposited here with Addgene for distribution to the research community. With... Endogenous Gene Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human... with endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure...

... distribution via Addgene. For a full list of Addgene's viral vectors See Addgene's Current AAV inventory... are now available for distribution at Addgene: Penn ID Addgene ID Name Function PI AV-1-49531P 100040...Penn Vector Core and Addgene partnership to provide AAV viral preps, includes listing of transfer status...Pennsylvania Vector Core (Penn Vector Core) and Addgene previously partnered to provide high-quality AAV...be helpful to the scientific community. Penn ID Addgene ID Name PI AV-1-18916P 18916-AAV1 AAV-FLEX-rev-...CI.TurboRFP.WPRE.RBG James M. Wilson Please contact help@addgene.org with any questions. Return to top...

... switches available in these tables, refer to Addgene's Optogenetics Guide . Interested in more news about... optogenetics research? Find related posts on Addgene's blog. Microbial Opsin Plasmids Browse, sort, or...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...untagged SpCas9 (Addgene 41815 or #44719 ) in combination with gRNA_AAVS1-T2 (Addgene #41818) or using... the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which expresses an untagged Cas9 AND ...containing homology arms and EGFP are available at Addgene. Do you have suggestions for other plasmids that...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Addgene lentiviral packaging, envelope, and transfer plasmids..., envelope, and transfer plasmids available at Addgene. Read our Lentiviral Vector Guide for more information... Additional Resources Lentiviral Vector Guide Addgene’s Viral Service Lentiviral Pooled Libraries All ...Viral Vectors 101 eBook Biosafety Resource Guide Addgene blog: Tips for Titering Your Lentiviral Preps Content...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

... the thousands of entry vectors deposited with Addgene), it can be rapidly shuttled into any compatible...project has been completed via Gateway cloning. Addgene's collection contains thousands of Gateway donor...to assemble before you get started. Check out Addgene's website for easy-to-use MoClo and Golden Gate ...Created with BioRender.com. Additional Resources Addgene Webpages Sequencing Primers Modular Cloning (MoClo...Perfect Plasmid Backbone Genome Eengineering Guide Addgene Protocols Plasmid cloning by restriction digest...ligation Gibson assembly Ligation independent cloning Addgene Blogs Plasmids 101: Restriction Cloning Plasmids...

...Overview of Addgene's educational resources, including eBooks, science guides, videos, and protocols ... content , including protocols, tips on using Addgene’s materials, and career advice. Playlists include...Protocols Careers How-to Blog Read about plasmids in Addgene’s repository , and related molecular biology techniques...

...article in which they were created, and include Addgene in the Materials and Methods of your future publications... this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your Reference section: ...

...start? Need a quick reference? Addgene's eBooks are here to help!... Addgene's eBook Collection Not sure where...where to start? Need a quick reference? Addgene's eBooks are here to help! 0 of 0 Selected Download Select...

... Though Addgene mainly uses next-generation sequencing (NGS) for plasmid verification, Addgene has used.... These lists are provided for your reference, Addgene does not distribute primers. To identify primers...additional annotated primers. You can also use Addgene’s protocol to design your own primers . Still have...plasmids, and recombinant DNA, please consult Addgene's Molecular Biology Reference page . Universal Sequencing...best primer for your plasmid? Email us at help@addgene.org . For additional information on molecular biology...

...Viral Vectors 101 eBook Addgene's Biosafety Resources Resources on Addgene's Blog An Introduction to Adenovirus... References Resources on addgene.org Adenoviral Plasmids Available at Addgene AdenoBuilder Toolkit Viral...information on cloning and working with plasmids, visit Addgene’s Molecular Biology Reference . AdEasy™ AdEasy™,...construct is then transformed into AdEasier-1 cells (Addgene #16399) , which are BJ5183 E. coli cells containing...viruses. The adenoviral backbone plasmid pAdEasy-1 (Addgene #16400) is suitable for most purposes. For especially...especially long transgenes, the use of pAdEasy-2 (Addgene #16401) can increase the capacity of the rAdV vector...best practices with regard to AdV research. See Addgene’s Biosafety Resource Guide for more information ...

...Selectable Marker Publication Additional Resources Addgene Kits MoClo-YTK Yeast Gateway Kit Multiplex Yeast...Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink Lab Materials Kurt Thorn Lab...

...AAV size limitation . Browse Addgene's in-stock AAV preps or try Addgene's Packaged on Request service....appropriate left and right homology arms. See Addgene's HDR blog post for more details. In some cases,...plasmid in question (see CRISPR protocols from Addgene depositors ). Browse empty gRNA expression vectors...you may need to look into other options. Browse Addgene's collection of CRISPR plasmids . Delivery Using...conducting genome-wide screens using CRISPR . Browse Addgene's collection of in-stock lentiviral preps . AAV ...

...Plasmids Zhang Lab CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about...lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse the Addgene GeCKO Library CRISPR page for more information....screening. For more information, please visit the Addgene SAM library page . Targeted gene activation using...mammalian endogenous gene activation using SAM: Addgene plasmids #61422 - #61424 were designed for transient...Contains BbsI sites for insertion of guide sequences Addgene plasmids #61425 - #61427 are intended for lentivirus...

...AAV size limitation . Browse Addgene's in-stock AAV preps or try Addgene's Packaged on Request service....appropriate left and right homology arms. See Addgene's HDR blog post for more details. In some cases,...plasmid in question (see CRISPR protocols from Addgene depositors ). Browse empty gRNA expression vectors...you may need to look into other options. Browse Addgene's collection of CRISPR plasmids . Delivery Using...conducting genome-wide screens using CRISPR . Browse Addgene's collection of in-stock lentiviral preps . AAV ...

...secreted proteins from a collaboration between Addgene and IPI....Other Markers Antibody Collection page to browse Addgene’s full catalog of antibodies that target epitope...

...the Library Once you receive your library from Addgene, check the library information to see if it should...crucial for conducting pooled library screens. Addgene does not offer next-generation sequencing services...our Pooled Library Amplification Protocol for Addgene's generalized library amplification protocol. Read...

...associated with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes ready-to-use...these antibodies are available at Addgene. AAV Viral Service : Addgene provides a viral service to help...Cre-dependent recombination Ofer Yizhar Antibodies Addgene distributes ready-to-use recombinant antibodies...application-specific validation and quality control by Addgene as well as by our trusted partner labs. Learn more...help facilitate scientific research. Addgene's AAV are made from a selected set of plasmids that are currently...

...A list of Addgene plasmids for use in synthetic biology applications, or from synthetic biology labs....to see added to this list, please email help@addgene.org . Synthetic Biology Plasmids Search the table...

...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently...