We narrowed to 967 results for: lat
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TypeCollection... in the collection have been developed using a platform (Link opens in a new window) based on yeast display...of highly conserved proteoglycans crucial for regulating cell signaling during development. The IPI collection...
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Optogenetics Plasmid Collection
TypeCollection...optical switch tools to activate, inhibit, and manipulate cellular processes....in more news about optogenetics research? Find related posts on Addgene's blog. Microbial Opsin Plasmids... -
TALEN Engineering
TypeCollection...CRISPR plasmids The Joung Lab has developed three platforms for engineering TAL effector repeat arrays of ...al., Nat Biotechnol 2012). All three of these platforms assemble TAL effector repeat arrays on a single... -
AAV for Neuronal Tracing
TypeCollection...core idea of the system is to infect the cell population of interest with a deletion-mutant tracing virus... only infect a genetically specified neuronal population. This is done using the envelope protein of (... -
Viral Vector Guides and Plasmids
TypeCollection...guides and plasmids available at Addgene and other related resources....referred to as just retroviral vectors, have a relatively high transduction efficiency and only infect ... -
Retrovirus Plasmids
TypeCollection...for the addition of WRE, a post-transcriptional regulatory element that increases export to the cytoplasm...IRES-mCherry. Dario Vignali 27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA. Expresses rtTA. See Zuber... -
Protocol - How to Streak a Plate
TypeProtocol... and Isolating Bacteria on an LB Agar Plate You may also like... Making LB Agar Plates Bacterial Transformation... last section of the plate, to create streak #3. Incubate plate with newly plated bacteria overnight (... DNA from it, you will want to isolate an individual clonal population (single colony) of bacteria from...single colony from a freshly streaked agar plate to inoculate a bacterial culture for DNA purification ...explains how to isolate a single bacterial colony by streaking it onto an LB agar plate. Last Update: Feb... LB agar plate (with appropriate antibiotic) Bacterial stab Procedure Obtain an LB agar plate with appropriate... one edge of the plate. Then streak horizontal lines in another section of the plate, and then diagonal... -
Microbiology Resources
TypeCollection...in accordance with export laws and biosafety regulations. Looking for E. coli or yeast plasmids? Addgene...Resources : Browse plasmids, from networks and gene regulation to sensing and signaling, to perform your synbio... -
Allen Institute for Cell Science Plasmid Collection
TypeCollection...101783 MAP1LC3B-mEGFP AICSDP-25 mEGFP Autophagy-related protein LC3 B Autophagosomes 101784 SLC25A17-mEGFP...junctions 107579 RAB5A-mEGFP AICSDP-39 mEGFP Ras-related protein Rab-5A Endosomes 107580 AAVS1-mTagRFPT-... -
Protocol - How to Inoculate a Bacterial Culture
TypeProtocol... Protocols Inoculating a Liquid Bacterial Culture Inoculating a Liquid...culture bacteria in the lab. LB agar plates are frequently used to isolate individual (clonal) colonies of ...necessary to isolate enough plasmid DNA for experimental use. The following protocol is for inoculating an overnight...You can now isolate your plasmid DNA from the bacterial culture by following the Isolating Your Plasmid...your LB agar plates are not fresh, you should streak your bacteria onto a new LB agar plate before growing...Additional Protocols Streaking and Isolating Bacteria on an LB Agar Plate Creating Bacterial Glycerol Stocks... for Inoculating a Bacterial Culture Protocols... -
Protocol - Over-Agar Antibiotic Plating
TypeProtocol... on LB Agar Plate Over-Agar Antibiotic Plating You may also like... Making LB Agar Plates Bacterial Transformation...device that fits the plate. Incubate plates at 37 ℃ for 18 hours. Day 2 Observe plates for colony formation...concentrations of carbenicillin plated over-agar. Control Plate with No Carbenicillin Plate shows a lawn of E. coli...Carbenicillin plated over-agar Plate shows less individual colonies than the 1 mg/mL plate and effective...purpose, provided that they fit your petri plate. Incubate the plate at room temperature for at least 30 minutes...selection. 150 µL of 0.1 mg/mL Carbenicillin plated over-agar Plate shows a lawn of E. coli and no apparent...selection. 150 µL of 1 mg/mL Carbenicillin plated over-agar Plate shows several individual colonies and effective... -
The Pleiades Promoter Project
TypeCollection...Plasmids from the Pleiades Promoter Project - A regulatory toolbox of MiniPromoters to drive selective expression...., Wasserman, W. W., Simpson, E. M. (2010). A regulatory toolbox of MiniPromoters to drive selective expression... -
Neurodegeneration Plasmid Collection
TypeCollection... plasmids for research related to Alzheimer's Disease (AD), Amyotrophic Lateral Sclerosis (ALS), Frontotemporal...Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia...Alzheimer's Zena Werb 19228 pBS ALS2 ALS2 T7 Primary lateral sclerosis juvenile Cliff Tabin 19457 pcDNA5/FRT...pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos Mourelatos 51146 DsRed-p150 217-548 DCTN1 CMV ALS, motor... -
Synthetic Biology - Browse Plasmids
TypeCollection...Engineering Browse the list of available plasmids related to synthetic biology below. If there are plasmids... -
Neuroscience
TypeCollection... recombinant antibodies targeting neuroscience-related proteins.... -
TREAT-AD Plasmid Collection
TypeCollection...targets that are valued opportunities for further translational investment. All resources and high-quality research... -
Zinc Finger Consortium Reagents
TypeCollection...for expressing zinc finger FokI nuclease fusions Related Genome Engineering Technologies TALEN Engineering... -
CRISPR Plasmids - dCas9-FokI
TypeCollection...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ... -
iPSC Neurodegenerative Disease Initiative Plasmid Collection
TypeCollection...ongoing project of the Center for Alzheimer’s and Related Dementias (CARD) (Link opens in a new window) at... -
CRISPR Plasmids - Single-Strand Break (Nick)
TypeCollection...Nickase mutants can also be used with a repair template to introduce specific edits via homology-directed...