...collection of synthetic biology plasmids for use in plants....collection of synthetic biology plasmids for use in plants. Plant Plasmids Search the table by keyword or ...

... TALengineering Reagents TALEN Expression Vectors TALEN Expression Vectors...This page lists plasmid vectors into which DNA fragments encoding TAL effector repeat arrays (assembled...

...cell types. Zinc finger arrays created using ZFC reagents for either OPEN or modular assembly can be easily... cells or zebrafish) pDW1775 (for expression in plants) Vectors encoding heterodimeric FokI nuclease domains...

...publications, focused on somatic variants found in lung adenocarcinoma as well as variants found across cancer types...

... , which also includes other types of affinity reagents such as scFvs, DARPins, Fabs, and secondary antibodies...addgene.org to help us improve our resources! Acknowledgments This project is supported by a NIH BRAIN Initiative...

...CRISPR plasmids. Each point represents a single cell, and each color represents the population obtained with...

...plasmids related to metabolic pathways and auxiliary components. Networks and Gene Regulation Browse plasmids...both naturally occurring and synthetic regulatory elements, including promoters, terminators, repressors,...

...γ-retroviruses. γ-Retroviruses can package relatively high amounts of DNA (up to ∼8 kb), and infection results in ...immortalized cells (see Weinberg Lab page for resistance variants) Weinberg 1773 pBABE-hygro-hTERT MoMLV Expression...

...’s recommended applications. The specific QC experiments performed varies for each antibody catalog item... mammalian cells. To learn which specific QC experiments were performed on your lot, please email help...

...Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

... Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

...lysosome biogenesis. mTORC2 does not respond to nutrients, but it is activated by growth factors, including... to find available plasmids for the gene. For components with multiple isoforms or subunits, individual...

...transcriptase. Engineering of the PE enzyme and related components have helped to improve the efficiency of editing... for expression in mammalian systems, bacteria, plants, and Drosophila. Mammalian ID Plasmid Gene/Insert...

...attractive for imaging and fluorescence microscopy experiments. The C. elegans genome is well studied and hundreds...plasmids that facilitate detection of genome-editing events. Developed in Craig Mello's lab and described in...

...Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

... Similar in principle to the ZF nuclease, the components of the array recognize individual nucleotides...Gate cloning method to assemble “multiple DNA fragments in an ordered fashion in a single reaction.” Using...

... TALengineering Reagents TALEN Zebrafish Reagents TALENs for Endogenous Zebrafish Gene...

...initial version of EvolvR has an editing window of ~17 nt from the nick site, which was expected since the ...generating novel genetic variants in bacteria. Read on to learn about the key components of EvolvR and its potential...library used. EvolvR: key components The EvolvR system has two key components: the error prone PolI3M that...scale by screening diverse libraries of genetic variants. Several methods for generating mutant libraries...protein-protein interactions, investigate non-coding segments of the genome, or engineer microbes to perform...to start EvolvR-ing your targeted mutagenesis experiments? Find EvolvR plasmids at Addgene! References...

...create cell lines with endogenously-tagged gene variants or browse Addgene’s entire collection of Stem ...Disease-Specific Phenotypes of Motor Neurons from Human ALS Patients. Cell Rep. 2016 Jan 5;14(1):115-28. Zhang Adult...

...doi.org/10.1016/j.cell.2013.08.021 Waters CA, Strande NT, Pryor JM, Strom CN, Mieczkowski P, Burkhalter MD...regardless of end structure, and dictate the major events of the pathway: Broken ends are recognized by ... probably accounting for less than 5% of repair events. However, the products of accurate repair are easily...Yan et al., 2020). NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR...