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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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In Category

We narrowed to 1,020 results for: nts

Showing: 101 - 120 of 1020 results

  1. E11 Bio PRISM Collection

    Type
    Collection
    ...part of an integrated pipeline, but individual components can be used separately for additional applications...listed in Park & Sheridan et al., 2025). These reagents have been used in expansion microscopy workflows...

  2. Synthetic Biology - Overview

    Type
    Collection
    ...plasmids related to metabolic pathways and auxiliary components. Networks and Gene Regulation Browse plasmids...both naturally occurring and synthetic regulatory elements, including promoters, terminators, repressors,...

  3. Retrovirus Plasmids

    Type
    Collection
    ...γ-retroviruses. γ-Retroviruses can package relatively high amounts of DNA (up to ∼8 kb), and infection results in ...immortalized cells (see Weinberg Lab page for resistance variants) Weinberg 1773 pBABE-hygro-hTERT MoMLV Expression...

  4. Antibody Production

    Type
    Collection
    ...’s recommended applications. The specific QC experiments performed varies for each antibody catalog item... mammalian cells. To learn which specific QC experiments were performed on your lot, please email help...

  5. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

  6. CRISPR Plasmids - Yeast

    Type
    Collection
    ... Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

  7. mTOR Pathway

    Type
    Collection
    ...lysosome biogenesis. mTORC2 does not respond to nutrients, but it is activated by growth factors, including... to find available plasmids for the gene. For components with multiple isoforms or subunits, individual...

  8. Worm Expression Resources

    Type
    Collection
    ...attractive for imaging and fluorescence microscopy experiments. The C. elegans genome is well studied and hundreds...plasmids that facilitate detection of genome-editing events. Developed in Craig Mello's lab and described in...

  9. Promega Plasmid Collection

    Type
    Collection
    ...fluorophore, as the energy acceptor. Live-cell reagents allow you to detect PPI induction and inhibition...inhibition in real time using full-length proteins or fragments. NanoBRET target engagement (TE) assays (Link ...

  10. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

  11. TALEN Guide

    Type
    Collection
    ... Similar in principle to the ZF nuclease, the components of the array recognize individual nucleotides...Gate cloning method to assemble “multiple DNA fragments in an ordered fashion in a single reaction.” Using...

  12. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-...Addgene. Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM, McCaw ZR, Aryee MJ, Joung JK...The number of off-target cleavage events for the VQR and VRER variants is similar to wild-type SpCas9, ...reported the development of near-PAMless Cas9 variants. These variants, named SpG and SpRY, can target NGN PAMs...the number of CRISPR reagents continues to grow, so too will the number of reagents available through Addgene...limitation: 1) the use of novel S. pyogenes Cas9 variants with varying PAM sequences, 2) the use of Cas9...positive selection screens in bacteria to identify mutants of S. pyogenes Cas9 that were able to cleave target...

  13. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...prepared in large amounts and frozen for future use or directly ligated with the annealed 24 nt oligos to generate...the spacer(20nt) on pEcgRNA plasmid as only two 24 nt oligos are required and the BsaI linearized pEcgRNA...not function properly in E. coli BL21(DE3) as transformants failed to grow after the transformation of pTargetF... This would result in the lack of recovered transformants. Our speculation motivated us to update the ...system While the above systems are great for experiments that require only single chromosomal integration...

  14. Plasmids for Stem Cell Research

    Type
    Collection
    ...create cell lines with endogenously-tagged gene variants or browse Addgene’s entire collection of Stem ...Disease-Specific Phenotypes of Motor Neurons from Human ALS Patients. Cell Rep. 2016 Jan 5;14(1):115-28. Zhang Adult...

  15. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... to top Fluorescent Timers Fluorescent protein variants that change their fluorescence from the blue to...protein with different colors as needed for your experiments. Learn more about the flexibility of self-labeling...

  16. Genetic Code Expansion

    Type
    Collection
    ... to Study Protein Phosphorylation Rinehart Lab reagents tRNA/Synthetase Pairs Bacterial Strains Codon ...designing, implementing, or troubleshooting GCE experiments in your research, you can get advice from GCE...

  17. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...more important because you have to target within ~30 nt of your proposed edit, which means there are so few...base editors, the intended edit must be in a 5 - 10 nt window relative to the PAM, and bystander edits are...SaCas9, NmeCas9, Cas12a enzymes, and engineered variants thereof offers additional PAM options that can...HDR for introducing edits. The same locational constraints are even more exquisite for the so-called base... JG (2020) Design and analysis of CRISPR–Cas experiments. Nat Biotechnol 38:813–823 . https://doi.org/...

  18. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ...acids long, has optimal sgRNA spacer length of 22-25 nts, and is thermostable between 31-100°C. They published...novel organisms and enzymes from these extreme environments. Gearing up for sample collection at the hot...temperature readings using a heat gun, and pH measurements. We collected small water and sediment samples...Cas9 be useful? It can enable applications where reagents must be stored at elevated temperatures for long...

  19. Neuroscience

    Type
    Collection
    ...addgene.org to help us improve our resources! Acknowledgments This project is supported by a NIH BRAIN Initiative...

  20. Synthetic Biology - Metabolism

    Type
    Collection
    ...biology plasmids related to metabolic pathways and components. Examples Include: Biofuels Natural Product Pathways...
Showing: 101 - 120 of 1020 results