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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,023 results for: nts

Showing: 101 - 120 of 1023 results

  2. All Antibodies

    Type
    Collection
    ... , which also includes other types of affinity reagents such as scFvs, DARPins, Fabs, and secondary antibodies...addgene.org to help us improve our resources! Acknowledgments This project is supported by a NIH BRAIN Initiative...

  4. Mammalian RNAi Tools

    Type
    Collection
    ...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...available. To find plasmids containing other RNAi components, like argonaute, please search our full site ...

  5. Synthetic Biology - Overview

    Type
    Collection
    ...plasmids related to metabolic pathways and auxiliary components. Networks and Gene Regulation Browse plasmids...both naturally occurring and synthetic regulatory elements, including promoters, terminators, repressors,...

  6. Retrovirus Plasmids

    Type
    Collection
    ...γ-retroviruses. γ-Retroviruses can package relatively high amounts of DNA (up to ∼8 kb), and infection results in ...immortalized cells (see Weinberg Lab page for resistance variants) Weinberg 1773 pBABE-hygro-hTERT MoMLV Expression...

  7. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ...acids long, has optimal sgRNA spacer length of 22-25 nts, and is thermostable between 31-100°C. They published...novel organisms and enzymes from these extreme environments. Gearing up for sample collection at the hot...temperature readings using a heat gun, and pH measurements. We collected small water and sediment samples...Cas9 be useful? It can enable applications where reagents must be stored at elevated temperatures for long...

  8. Antibody Production

    Type
    Collection
    ...’s recommended applications. The specific QC experiments performed varies for each antibody catalog item... mammalian cells. To learn which specific QC experiments were performed on your lot, please email help...

  9. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

  10. CRISPR Plasmids - Yeast

    Type
    Collection
    ... Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

  11. mTOR Pathway

    Type
    Collection
    ...lysosome biogenesis. mTORC2 does not respond to nutrients, but it is activated by growth factors, including... to find available plasmids for the gene. For components with multiple isoforms or subunits, individual...

  12. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...transcriptase. Engineering of the PE enzyme and related components have helped to improve the efficiency of editing... for expression in mammalian systems, bacteria, plants, and Drosophila. Mammalian ID Plasmid Gene/Insert...

  13. Worm Expression Resources

    Type
    Collection
    ...attractive for imaging and fluorescence microscopy experiments. The C. elegans genome is well studied and hundreds...plasmids that facilitate detection of genome-editing events. Developed in Craig Mello's lab and described in...

  14. Promega Plasmid Collection

    Type
    Collection
    ...fluorophore, as the energy acceptor. Live-cell reagents allow you to detect PPI induction and inhibition...inhibition in real time using full-length proteins or fragments. NanoBRET target engagement (TE) assays (Link ...

  15. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

  16. TALEN Guide

    Type
    Collection
    ... Similar in principle to the ZF nuclease, the components of the array recognize individual nucleotides...Gate cloning method to assemble “multiple DNA fragments in an ordered fashion in a single reaction.” Using...

  18. Plasmids for Stem Cell Research

    Type
    Collection
    ...create cell lines with endogenously-tagged gene variants or browse Addgene’s entire collection of Stem ...Disease-Specific Phenotypes of Motor Neurons from Human ALS Patients. Cell Rep. 2016 Jan 5;14(1):115-28. Zhang Adult...

  19. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...initial version of EvolvR has an editing window of ~17 nt from the nick site, which was expected since the ...generating novel genetic variants in bacteria. Read on to learn about the key components of EvolvR and its potential...library used. EvolvR: key components The EvolvR system has two key components: the error prone PolI3M that...scale by screening diverse libraries of genetic variants. Several methods for generating mutant libraries...protein-protein interactions, investigate non-coding segments of the genome, or engineer microbes to perform...to start EvolvR-ing your targeted mutagenesis experiments? Find EvolvR plasmids at Addgene! References...

  20. Genetic Code Expansion

    Type
    Collection
    ... to Study Protein Phosphorylation Rinehart Lab reagents tRNA/Synthetase Pairs Bacterial Strains Codon ...designing, implementing, or troubleshooting GCE experiments in your research, you can get advice from GCE...
Showing: 101 - 120 of 1023 results