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Showing: 181 - 210 of 362 results

  1. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...producing lentivirus in Lenti-X 293T cells using a polyethyenimine transfection protocol. I also recommend searching...incubation time, antibiotic conditions, and amount of polybrene. (psst…didn’t get your library pre-made from Addgene...

  2. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...applications, including detection of environmental pollutants, drug discovery, and disease diagnosis. As biosensors...electrochemical sensors for emerging environmental pollutants.” Front Chem. 2014; 2: 41. PubMed PMID: 25019067...

  3. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...sample is first amplified using RPA (recombinase polymerase amplification) or reverse transcriptase (RT)-...as well as health-related single nucleotide polymorphisms (SNPs) from human saliva. SHERLOCKv2 provides...

  4. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...exposed to specific UV wavelengths or chemicals (Polstein and Gersbach, 2015; Zetsche et al., 2015). These...e7 . https://doi.org/10.1016/j.cels.2018.04.011 Polstein LR, Gersbach CA (2015) A light-inducible CRISPR-Cas9...

  5. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...where they are incorporated into the growing polypeptide chain. One part of the tRNA contains the anticodon... codon for every amino acid across an entire polypeptide. Many researchers have created codon-optimization...

  7. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...Anuganti, M., Zhang, R., Lloyd, K., Jardine, J., Coppola, J., Meijers, R., Li, J., & Springer, T. A. (2024...2024.01.26.577394 Jo, M. H., Li, J., Jaumouillé, V., Hao, Y., Coppola, J., Yan, J., Waterman, C. M., Springer, T. A.,...

  8. CRISPR References and Information

    Type
    Collection
    ...donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED PDF 583.3 KB Zhang gRNA cloning CRISPR RNA...

  9. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...guide to working with Drosophila Terminators and PolyA Signals FLEx Vectors Resources at Addgene.org ...

  12. Caltech Systemic Capsids

    Type
    Collection
    ...Dimidschstein 192552 pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA CAG tdTomato Control Feng 163505 CN1390-rAAV-DLX2.0...

  15. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...thaliana dCas9 Activation of gene transcription Polstein et al., 2015 LACE CRY2 and CIB1 from A. thaliana...https://doi.org/10.1016/j.chembiol.2014.12.011 Polstein LR, Gersbach CA (2015) A light-inducible CRISPR-Cas9...

  16. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Bradley 3rd ~9 pairs/exon 9,508 Mouse CRISPR Proximal Poly(A) Site Knockout Library 190744 Knockout Mouse Bradley...

  18. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...chain constant domain (C L ) and the κ light chain polyA tail sequences (κ pA), a CMV promoter for heavy ... depending on the plasmid, followed by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody ...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...of assembling these multi-protein fusions or “polyproteams”, the Howarth lab created the MBPx-SpyCatcher...sequential additions have been made, the final polyproteam can be eluted using maltose. This procedure allows...

  23. Our New Antibody Mascot is....

    Type
    Blog Post
    ...head over to Twitter to vote on @addgene's Twitter poll. Voting is open until Feb. 22, 2022, and we'll unveil...

  25. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...Jensen New serotype pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA AAV9, PHP.eB Controls Feng New viral service, ...

  28. Reflections and Looking Ahead

    Type
    Blog Post
    ...R. A., Virk, H., Laflamme, C., Houston, D. W., Polinski, N. K., Meijers, R., Levey, A. I., Saper, C. B...
Showing: 181 - 210 of 362 results