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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,010 results for: CAL

Showing: 121 - 140 of 1010 results

  1. Adenovirus Plasmids

    Type
    Collection
    ...termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined into a DNA molecule...

  2. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator...

  3. Antibody Plasmid Collection

    Type
    Collection
    ...and time. An alternative approach is to use synthetically produced recombinant antibodies, created by ...

  4. Worm Expression Resources

    Type
    Collection
    ...C. elegans strains. CRISPR plasmids from John Calarco's lab as described in Heritable genome editing in...

  6. p53 Pathway

    Type
    Collection
    ... of p53 splice variants in human malignancy: a clinical perspective. Surget S, Khoury MP, Bourdon JC. ...

  7. Arf GTPase Family

    Type
    Collection
    ...regulators of membrane traffic, they also play critical roles at cilia, centrosomes, the plasma membrane...

  8. Open Enzyme Collection

    Type
    Collection
    ...pOpen-BovDNTT Bovine DNTT 165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top Additional...

  10. Ras Pathway

    Type
    Collection
    ... removal of a phosphate group. Under normal physiological conditions GTPases play prominent roles in signaling...

  11. TALEN Plasmids and Kits

    Type
    Collection
    ... domain of a TALE, along with strong nuclear localisation tags and repress transcription initiation when...

  15. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ...example image). Point Scanning Confocal Microscopy Point Scanning Confocal Microscopy was the first fluorescence... microscopy technique to incorporate optical sectioning. Optical sectioning refers to the ability to extract...detector. The pinhole is specifically placed to only allow light from the focal plane to reach the detector...excluded. Parallelized Confocal Microscopy (Spinning Disk) Parallelized Confocal Microscopy (Spinning Disk...drawbacks of widefield and confocal microscopy. First, widefield and confocal microscopy project excitation...multiple focal planes of a sample may also be useful for providing fast imaging along with optical sectioning...respective focal plane. In sufficiently thin samples, deconvolution can outperform optical sectioning ...

  16. A Control for All Seasons

    Type
    Blog Post
    ...another critical control in biological experiments. Replicates can be technical or biological and are ... inclusion of biological replicates such as multiple mice in each group and C, technical replicates or...recorded would be due to natural biological variation. This information is critical for correctly interpreting... accuracy and therefore the statistical power of an experiment. Technical replicates are repeated measurements...meaningful results.  Biological replicates are distinct samples that are treated identically, used to assess...binds specifically to its target through the antigen binding site but also binds non-specifically to other...binds specifically to its target through the antigen binding site but also binds non-specifically through...

  17. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...report physical titer, which is measured by qPCR.   Physical titer is typically calculated by two ...particle typically contains one viral genome. Other than a theoretical maximum value, physical titers don... you? Physical titer: the concentration of viral particles containing viral genomes. Physical titers ...facilities report the physical titer of a viral solution (Figure 2). Because physical titers are used for...that can transduce cells. Infectious titers are typically quantified by cell transduction assays. Wild-type... AAV2 has been reported to have a near-perfect physical-to-infectious particle ratio of 1:1 (Zeltner et...for recombinant AAV2, the same study reported a physical-to-infectious particle ratio of 50:1 (Zeltner ...

  18. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ... John H. McDonald. "A practical guide to evaluating colocalization in biological microscopy." American...subcellular localization of your protein of interest The first step in analyzing the localization of your ...organs, cells comprise compartments and structures, called organelles. Take a sneak peak inside a cell with... proteins of interest and examine them using biochemical methods thus removing the context of the cell...living cell. Analyzing differences in protein localization and transport between healthy and diseased states...for example, validate that the fusion protein localizes properly by comparing the signal from the fusion...protein and see who it is hanging out with via colocalization - aka “are they close?” After tagging your ...

  19. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...and sensing electrical potential. Thankfully, there is a tool to measure these electrical events! Voltage...essential to their use as biological tools. Why Use Voltage Indicators? Electrical signals are integral to...Sensors This system consists of a genetically expressed enzyme, typically on the cell surface, which can ...the chemical factor, of a naturally occurring voltage indicator. To give an example, a genetically encoded... tag, cage, uncage, and trap the chemical fluor with the genetically encoded component of the system to...needs to be cell type specific as well as specifically localize to the membrane, otherwise they aren’t all... all that useful. The cellular localization component is typically handled by either the fluorescent component...

  20. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...interaction between a bisarsenical dye ligand (e.g., FlAsH, ReAsH) and a short genetically encoded tetracysteine...systems consist of a genetically encoded enzyme variant tag that reacts specifically and irreversibly with... as intact tissue. Some chemical labeling strategies such as the bisarsenical dye (Figure 1a) and FAP ...Ronald T. Raines. "Bright ideas for chemical biology."ACS chemical biology 3.3 (2008): 142-155. PubMed... at the Janelia Research Campus, Howard Hughes Medical Institute. Chemistry is dead, long live chemistry...fluorescent protein (GFP) sparked a renaissance in biological imaging. Suddenly, cell biologists were no longer...synthetic fluorophores. Add a dash of DNA with an electrical jolt and cells become perfectly capable of synthesizing...
Showing: 121 - 140 of 1010 results