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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,105 results for: Ide

Showing: 121 - 140 of 1105 results

  1. p53 Pathway

    Type
    Collection
    ...subunits, individual links to each gene page are provided below. Color is used for clarity and does not ...subunits, individual links to each gene page are provided below. Symbol Name 14-3-3-σ Stratifin Apaf-1 Apoptotic...apoptosis inducing protein 1 p53R2 p53 inducible, ribonucleotide reductase M2 B (RRM2B) PAG608 Zinc finger, ... PIDD p53-induced death domain protein 1 PIGs Etoposide induced 2.4 PIRH-2 Ring finger and CHY zinc finger...

  2. Viral Production

    Type
    Collection
    ...Sequencing results are analyzed to confirm the identity and integrity of the viral genome and the absence...Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver...negative staining. Empty vector particles can be identified after negative staining and appear darker than...to the cleared viral preparation pool. Proviral Identity Confirmation and Sterility All of our lentiviral...

  3. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...the time of shipment. If any discrepancies are identified during this analysis, you will be notified by... Addgene performs QC measures which verify the identity of the viral vector, but not the function. Upon... years of the order being shipped, Addgene can provide the QC validation data performed on the material...

  4. Fluorescent Proteins: FRET

    Type
    Collection
    ...between fluorescent proteins connected via flexible peptide linkers mCerulean, mVenus, and Amber (mVenus Y67C...mTurquoise2-mVenus(L68V) Goedhart et al. (2012) Structure-guided evolution of cyan fluorescent proteins towards ... S., Zhang, S., Lin, M. Z., & Chu, J. (2016). A Guide to Fluorescent Protein FRET Pairs . Sensors , 16...

  5. Plasmids for Stem Cell Research

    Type
    Collection
    ... to decide which method to use. Addgene's blog post Delivery Methods for Generating iPSCs provides an ... also have the unique potential to continuously divide while maintaining an undifferentiated state. Stem...

  6. mTOR Pathway

    Type
    Collection
    ...subunits, individual links to each gene page are provided below. Color is used for clarity and does not ...subunits, individual links to each gene page are provided below. mTORC1 Complex Symbol Name 4E-BP Eukaryotic...

  7. Zinc Finger Consortium Reagents

    Type
    Collection
    ...additional pages describing these reagents are provided below: Zinc Finger Genome Engineering Reagents...

  8. NETRF

    Type
    Collection
    ...Carcinoid Tumors Bradley Bernstein Broad Institute Identifying Altered Epigenetic States and Drivers in Intestinal...

  9. MAPK Plasmids

    Type
    Collection
    ...also like... Human Kinase ORFs Genome Engineering Guide Empty-Backbone Resource Mitogen-activated protein...

  10. AAV Viral Preps

    Type
    Collection
    ...transduce many mammalian cell types. AAV is used in a wide range of experiments, both in vitro and in vivo....

  11. Plasmid Collections

    Type
    Collection
    ...together based on type or application. Browse our guides to find the plasmids and science information you...

  12. Synthetic Biology - Overview

    Type
    Collection
    ... community. To deposit, view our SynBio Deposit Guide or start our online deposit process . Browse SynBio...

  13. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...more attractive candidate for that ideal job. How can you identify your transferable skills? PLoS One ...collaboration and fail to identify it as a personal strength until it is identified by another person. Similar...when a new venture presents itself even outside of work, consider challenging yourself, take the opportunity...All while still paying bills and having a life outside my job. The same can be said about teamwork, communication...transferable skills so important? It is important to identify which transferable skills you possess because ... and making connections, avoid falling into an identity trap where you assume your technical skills define...wants to hear a specific time that you thought outside the box to solve a problem. When they ask about...

  14. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...high fidelity ligase—and when is an alternative ligase a better choice? And what is ligase fidelity anyway...are "higher fidelity") better than T4 DNA Ligase (21, 25, 26). Despite this higher fidelity, Taq DNA Ligase...annealed complementary regions. Why use a high fidelity ligase? Numerous applications have been developed...developed to take advantage of the high fidelity of Taq and other thermostable DNA ligases. These applications...applications detect specific nucleotide sequences with high specificity and quantitative accuracy and even include... include profiling single nucleotide polymorphisms (SNPs) (5,12,13). For example, in the Ligase Detection...PCR amplified fragment) and treated with a high fidelity thermostable DNA ligase. If the target sequence...

  15. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    ... contain the Addgene ID # and the information necessary to indicate that this ID is managed by Addgene...within the Addgene catalog. These IDs allow you to unmistakably identify a plasmid available through Addgene...VQR-Blast, which has the Addgene ID #87155. The resolvable compact identifier format for lenti-Cas9-VQR is...will be assigned an Addgene ID #, RRID, and resolvable compact identifier. Addgene works hard to ensure...including plasmids. All plasmids at Addgene have unique ID numbers associated with them, which serve as unique...about each plasmid item. Given a specific Addgene ID # listed in a publication, you can quickly pull up...the “Articles Citing this Plasmid” section. Having IDs linked to these metadata and publications help to...

  16. CRISPR Guide Update

    Type
    Blog Post
    ...is proud to present our updated CRISPR Guide! The CRISPR Guide helps scientists understand how to use...incredible new CRISPR technologies developed and widely adopted in the past few years, as well as refreshing...refreshing the existing content. Access the CRISPR Guide here! Highlights include a much-anticipated prime... PRIME editing Please check out the updated guide for a great introduction to what CRISPR technologies...

  17. A Scientist's Guide to Ebola

    Type
    Blog Post
    ... viral attachment and fusion. Once inside a host cell, new evidence in PLoS Pathogens shows that the Ebola...week within two months. That said, Ebola is not considered a “terribly infectious disease” – infection occurs...via body fluids not through the air and that’s considered unlikely to change – but the illness has been...thought we’d do a little digging and assemble a rough guide to the science of Ebola and related resources. Ebola...

  18. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Other Promoter CMV T7 polH GAL Other Clear Filters ID Plasmid Name Gene Tags Promoter Disease PI 1177 p416...myc HIS SMN1 His, Myc CMV Spinal muscular atrophy Gideon Dreyfuss 71875 pET-Sac-Abeta(M1-42) APP T7 Alzheimer's...pFBD-BirA_LRRK2:2141-2527 LRRK2 His, Biotin acceptor peptide tag polH Parkinson's Cheryl Arrowsmith 210986 pHTN-HaloTag_C9orf72... found to match all requested criteria. Please consider using fewer filtering options. Browse all Neurodegeneration...

  19. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...share ideas. Ideas had over lunch are just as valuable! In 2019, Addgene implemented a company-wide New ...Transferable Skills Guide: Problem-Solving Transferable Skills Guide: Teamwork Transferable Skills Guide: Identifying...are still struggling with idea generation, try this thought exercise: Consider the issue as if there were...faster that way— but the idea behind the study holds. To generate novel ideas, start with an out-of-the-box...most prominent idea, and instead building an environment where everyone has their ideas heard.  So how...in which the goal is to generate unique ideas. Recognize ideas can come any time. Meetings shouldn’t be...to encourage the sharing of ideas, not to give people more work. Identify your enemies In Felicia Day...

  20. New Coomassie Protocol Video

    Type
    Blog Post
    ....    If this video is helpful, you can find our complete collection of protocol videos on the Addgene...antibody's purity? Check out our latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies...
Showing: 121 - 140 of 1105 results