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We narrowed to 152 results for: Tore

Showing: 121 - 140 of 152 results

  2. Cre-lox system

    Type
    Collection
    ...Cepko 13780 pNrl-Cre Cre-Myc, Expressed in rod photorecetor cells in the retina Nrl Mammalian Cepko 13962...

  5. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...LB agar plate can be stored at 4°C for a few weeks. However, if you want to store bacteria for a longer...long-term storage of plasmids. Although you can store your plasmid DNA at -20°C, many labs also create...cells alive. A glycerol stock of bacteria can be stored stably at -80°C for many years. Protocol Video ...long-term glycerol storage is unknown. Most labs store bacteria in 15-25% glycerol. You can prepare the.... Frozen tubes are hard to write on and samples stored for long periods at -80°C can lose labels stuck...

  6. Lentivirus Production

    Type
    Protocol
    ...sterile tubes. Store the tubes at -20 °C or -80 °C. After thawing, the solution can be stored at 4 °C for... of heat-inactivated FBS and 5 mL of glutaGRO. Store at 4 °C. Pro-Tip Different brands and lots of FBS...harvested media to a polypropylene storage tube and store at 4 °C between harvest. Centrifuge the viral supernatant....45 μm PES filter. The viral supernatant can be stored at 4 °C for several hours but should be aliquoted...aliquoted and snap frozen in liquid nitrogen and stored at -80 °C as soon as possible to avoid loss of titer...

  7. General Transfection

    Type
    Protocol
    ...filter. Aliquot 50–100 µL and store at -20 °C. Aliquots can be thawed and stored at 4 °C prior to use. Thawed...into sterile tubes. Store the tubes at -80 °C. After thawing the solution can be stored at 4 °C for up to...heat-inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C. Pro-Tip Different brands and lots of FBS...

  8. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...sterile 0.22 µm filter. Prepare 10 mL aliquots and store the solution at -20 °C. 1 mg/mL PEI-MAX Add 1 g ... through a 0.22 µm filter. Prepare aliquots and store at -20 °C until use. BCD TFX 1000 mL BalanCD HEK293...mL 200 mM Glutagro Do not add selective reagents Store at 4 °C until use. We suggest preparing fresh solutions...500 mL BalanCD HEK293 Feed 20 mL 200 mM Glutagro Store at 4 °C until use. We suggest preparing fresh solutions...our Recombinant Antibody Purification Protocol ). Store the supernatant at 4 °C until ready to use....

  9. AAV Production in HEK293 Cells

    Type
    Protocol
    ...into sterile tubes. Store the tubes at -80 °C. After thawing, the solution can be stored at 4 °C for up to... glutaGRO 11 mL of 200 mM L-alanyl-L-glutamine. Store at 4 °C. D1 + 0.1 M sorbitol (optional) : DMEM ... solution may separate into phases. Aliquot and store at 4 °C. Cell Lysis Buffer : 50 mM Tris HCl, 150....5 Filter sterilize through a 0.22 μm membrane. Store at 4 °C. Considerations Before You Start The health...

  10. Antibody Guide

    Type
    Guide
    ...antibodies can be stored at 4 °C for short-term storage. For long-term storage they should be stored at -20 °C...Antibodies conjugated to fluorophores should never be stored below 4 °C. Certain isotype subclasses, such as...complete. HRP-conjugated antibodies should not be stored in sodium azide, as it acts as an inhibitor to ...

  11. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...much virus as possible. Store at 4 °C for short term (2 weeks), or aliquot and store at -80 °C for long term...Sterilize by passing through a 0.22 μm filter and store at 4 °C. 1X PBS-MK buffer Dissolve 26.3 mg of MgCl...Sterilize by passing through a 0.22 μm filter and store at 4 °C. 0.1% Pluronic-F68 in PBS (A) Add 500 µL...

  12. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ... mM EDTA Store Solution I at 4°C Solution II - Denaturing Solution 0.2 N NaOH 1.0% SDS Store Solution ...acetate 23 mL glacial acetic acid 57 mL of dH 2 O Store Solution III at 4°C Grow 2 mL overnight cultures... room temperature before quantifying and using. Store DNA at 4°C. Tips and FAQ Plasmid purification kits...

  13. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    .... The media contains your lentiviral particles. Store at 4°C. m. Add 5 mL of fresh media containing antibiotics...shear the envelope of your virus. o. Virus may be stored at 4°C for a few days, but should be frozen at ...concentration of 100 μg/mL antibiotic. Pour plates and store at 4°C. Hexadimethrine Bromide (Polybrene) Prepare...stable at 4°C for several years. Protamine Sulfate Store protamine sulfate (MP Biomedicals catalog #194729... Sterilize by passing through a 0.22 μm filter. Store aliquots at -20°C. I.3. Warranty Information Addgene...

  14. Optogenetics Guide

    Type
    Guide
    ...Researchers have harnessed plant and bacterial photoreceptors to create protein systems controlled by light... and ePDZb1 as tags. pDawn/pDusk Blue-light photoreceptor for light-controlled gene expression. These ...fluorescent protein 400 UVR8 domains UVR8 plant photoreceptor protein that forms photolabile homodimers with...

  15. Chemogenetics Guide

    Type
    Guide
    ...phospholipase C, which releases intracellular calcium stores. There are currently three Gq DREADDs based on ... Stem Cell-Derived Neurons Repair Circuits and Restore Neural Function. Cell Stem Cell. 28:112-126.e6....

  17. Pouring LB Agar Plates

    Type
    Protocol
    ...material to reduce condensation. The plates are then stored at 4 ℃ until use. Once your plates have solidified...ready for use or storage at 4 ℃. You should not store your plates for longer than 1 month at any temperature...

  19. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...heat-inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C. Polyclonal stable cell pool: see our protocol... empirically, and that the cell population be monitored regularly for transgene expression. This protocol...

  20. Kit Free RNA Extraction

    Type
    Protocol
    ...quantification , which can be modified for RNA. Store your RNA sample(s) at -80 °C to prevent RNA degradation...DNA quantification , which can be modified for RNA.Store your RNA sample(s) at -80 °C to prevent RNA degradation...
Showing: 121 - 140 of 152 results