We narrowed to 363 results for: gne

1. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   ...uses Cre to trigger the inversion of two genes aligned head-to-head. Another variation, Brainbow 2.1, ...trigger the inversion of two tandem sets of genes aligned head-to-head and the deletion of one of the gene...

2. Transferable Skills Guide: Conflict Resolution

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   ...I really brought to the table was empathy, a willingness to listen, and an ability to remember the right... actively runs away from it. That can mean a willingness to make yourself temporarily uncomfortable for...

3. CRISPR 101: Validating Your Genome Edit

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   ... updated Nov 3, 2022 by Susanna Stroik. You’ve designed your gRNA and introduced it into your target cells...your desired edit. This silent mutation should be designed solely for restriction enzyme sensitivity or resistivity...

4. Site Directed Mutagenesis by PCR

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   ...mutated clones. Forward and reverse primers are designed to be complementary, but each primer may extend...and allows for the introduction of specifically designed mutations (not limited to frame shift mutations...

5. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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   ...enhance transcriptional activation. Zhang et al. designed multiple gRNAs to target the 5’ long terminal ...prevent reinfection of those cells. Kaminski et al. designed gRNAs to target the HIV-1 5’ and 3’ LTRs and expressed...

6. Viral Vectors 101: Optogenetic Tools

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   ... light in combination with retinal) variations designed to give researchers flexibility in using optogenetic...precise timing of the perturbation. Other tools are designed to be activated within a narrower band of wavelengths...

7. When Fidelity Matters: A frank discussion about ligase fidelity

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   ...possible NN pairs in the template. The probes were designed such that each possible pairing resulted in a ... using a capillary tube, probe DNA-immobilized magnetic beads and chemiluminescence detection." Analytical...

8. RNA Interference in Plant Biology: New Tools for an Old Favorite

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   ...Fluorescent reporter-based RNAi constructs have been designed and widely implemented in plants, such as in the...approach, myself and others in the Luis Vidali lab designed a new RNAi method. Instead of using a fluorescent...

9. Custom CRISPR Screens & the Green Listed Software

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   ...moment, 13 full genome SpCas9 knockout libraries designed by different academic labs, targeting mouse, human...descriptive transcriptomics data. Custom CRISPR screens designed to target differentially expressed genes can be...

10. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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    ...substance (fluorophore) that has absorbed light or electromagnetic radiation at another wavelength (Heinrichs,...signal-to-noise ratios. Certain dyes have been specifically designed for use in super-resolution microscopy techniques...

11. How to Deposit Your Plasmids with Addgene

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    ...the shRNA sequence. Empty backbone A plasmid designed for cloning a gene/insert into it. Empty backbones...intended to be used. For instance, if your plasmid is designed to allow you to produce large amounts of your ...

12. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

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    ...candidate will be hired for a role that is closely aligned with their education/experience, i.e. a research...sponsoring employer. On April 18, 2017, President Trump signed a "Buy American, Hire American" Executive Order...

13. Optogenetics + CRISPR, Using Light to Control Genome Editing

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    ...figure below. Their most successful design utilized Magnet photoswitchable proteins derived from the fungal...gene transcription Nihongaki et al., 2015 paCas9 Magnet proteins from N. crassa Split Cas9, Split Cas9 ...

14. CRISPR Plasmids - RNA Editing

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    ...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets....

15. Fluorescent Protein Guide

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    ... Medical School Fluorescent Biosensor Database designed by Eric Greenwald and the Jin Zhang Lab at UCSD...

16. CRISPR Plasmids - Double-Strand Break (Cut)

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    ...or search the tables below for CRISPR plasmids designed to introduce a DSB. Plasmids are available for...

17. DNA Service - Cloning Grade DNA

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    ...cloning grade DNA into cells? Cloning grade DNA is designed for use in cloning applications. Direct transfection...

18. Brzezinski Lab CRISPR Collection

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    ...VPR for activation. Usage Guide sequences can be designed using Benchling or using similar design tools ...

19. TALEN Engineering

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    ...laboratory for engineering TAL effectors, including designed TALENs, and assembly methods for REAL, REAL-Fast...

20. CRISPR Plasmids - Base Edit

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    ...protein (Mu- GAM). While many base editors are designed to work in a very narrow window proximal to the...