...Innovation (IPI). For these antibodies, IPI will be indicated as the Depositing Lab on the antibody webpage....cells and will interfere with conjugation. For applications such as these, the buffer can be removed by ...tissue panel in one of the antibody’s recommended applications. The specific QC experiments performed varies...order, ensuring each item ships safely and most economically. Actual shipping conditions will be shown in...

...compatible with multiple applications (different fluorophores, biotin, bead purification, blocking agents) ACP...-cell-permeant proteins. CLIP-tag CLIP-tag is identical in function to SNAP-tag but reacts with benzylcytosine...Specific labeling of cell surface proteins with chemically diverse compounds. J Am Chem Soc. 126(29):8896...

...advantages and disadvantages depending on the application. Firefly luciferase is considerably brighter ...luciferase-based plasmid systems for many different applications. Browse the Collection Highlights section to...use the red-shifted substrate Akalumine with significantly brighter signal and emission in the near-infrared...respectively, for increased usefulness in in vivo applications. LuxSit-i : An artificial luciferase desined...Malcolm Moore 51819 Syn-ATP Firefly (modified) CMV Optical reporter of presynaptic ATP Timothy Ryan 24348 ...

...transcription on the gene expression level without genetically altering the target loci. Thus, CRISPRi is a ...Plasmid Description 44246 pdCas9-humanized A catalytically inactive, human codon-optimized Cas9 expression...expression plasmid 44247 pdCas9::BFP-humanized A catalytically inactive, human codon-optimized Cas9-BFP fusion...codon-optimized Cas9 constructs 44249 pdCas9-bacteria A catalytically inactive bacterial Cas9 expression plasmid 44250...

...both dividing and non-dividing cells without a significant immune response. These viruses also integrate...divided among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode...with the plasmid that encodes the viral genome (typically called the transfer plasmid ). This table lists...included to monitor expression Parijs 11619 pLB 3rd Modification of pLL3.7; Genetic elements known to prevent...

...and Resources page have been used to indicate the Cas9 application the gRNA was designed to accomplish....upstream of a 5' NGG 3' PAM sequence). Which CRISPR application is this gRNA sequence compatible with? CRISPR...Gene Target Species Target Sequence Plasmid ID Application Cas9 Species PubMed ID Depositor OCT4 H. sapiens...be added to this table from any peer reviewed publication. To add validated gRNA sequences to our datatable...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled... supplemental materials from the Doyon Lab’s publication: Dalvai et al. Cell Rep. 2015 . Alternatively...programming sgRNA and HR donors for selectable genome modification. This system can be used to create C- and N-...following articles provide PCR templates for amplification of the tag (eg GFP, Flag, YFP, etc) and selection...

...Addgene plasmids for use in synthetic biology applications, or from synthetic biology labs.... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication...

...synthetic biology plasmids related to cell-to-cell communications and environmental sensing. Sensing and Signaling... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Gene/Insert Vector Type Promoter Tags PI Publication Do you have suggestions for other plasmids that...

... collection will grow as the IGVF community systematically perturbs the genome to assess the impact of...Addgene. ID Plasmid Description Vector Type PI Publication Additional Resources The IGVF Consortium (Link...opens in a new window) . Find IGVF Consortium publications on bioRxiv (Link opens in a new window) or PubMed...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...domain of ADAR2(E488Q) adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor...systems. Mammalian ID Plasmid Gene/Insert PI Publication Do you have suggestions for other plasmids that...

... has a unique 24 bp barcode that simplifies identification of the transcription factor isoform in pooled...used for screening transcription factors for applications such as cellular programming or cancer drug ...barcoded with a 24 bp sequence, to simplify identification during screening. Library ID PI Description...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Protocols gRNA Design Tools CRISPR Blog Posts Using catalytically inactive Cas9 (dCas9) fused to a fluorescent...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

...constructs also contain fluorescent labels that indicate if recombination has occurred, allowing for direct...Mammalian Sato 122961 pcDNA3.1_PA-Cre-Y324F Catalytically inactive PA-Cre CMV Mammalian Sato 123128 pET-His-Cre...excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre... this subtype of loxP plasmids, reporter genes indicate which cells have undergone Cre recombination. ...which reporters are initially OFF and then probabilistically ON or OFF following Cre recombination to maximize...

...reducing the time to experiments by removing the amplification and extraction steps required when one receives...determined by spectrophotometer. Other methods of quantification may yield slightly different DNA concentrations...Cloning grade DNA is designed for use in cloning applications. Direct transfection into cells is not recommended...

...Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source... in the Materials and Methods of your future publications. For your Materials and Methods section: "The...The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your...

...soluble recombinant molecules. The proteins are typically used in systematic protein interaction screens...benchmarked protein microarray-based platform for the identification of novel low-affinity extracellular protein...Sun Y, Gallagher-Jones M, Barker C, Wright GJ. Analytical Biochem. 2012 May 1;424(1):45-53....

...Scientists using these zinc finger arrays in a publication should notify [email protected] and...acknowledge NIH grant number R01 GM088040 in the publication. References Rapid 'open-source' engineering of...zinc-finger nucleases for highly efficient gene modification. Maeder ML et al. Mol Cell. . 2008. Jul 25. ...

...not indicate a specific relationship. p53 Pathway Color is used for clarity and does not indicate a specific... of p53 splice variants in human malignancy: a clinical perspective. Surget S, Khoury MP, Bourdon JC. ...

...libraries to screen for genes involved in specific biological processes. For more information, see our Guide...Function Antibiotic Resistance Activity PI 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human ... Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused to a transactivator...