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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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In Category

We narrowed to 987 results for: nin

Showing: 121 - 140 of 987 results

  1. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...mediate the integration of a large DNA payload containing an attB target site into a genomic attP site....the expression of one gene while simultaneously turning on another. The FLEx switch system takes advantage... target location for integration of a plasmid containing a gene of interest and another FRT site. Flp ...

  2. KLF Research Plasmids

    Type
    Collection
    ...fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is the presence of three...resource on Addgene. If you have created plasmids containing KLF, we encourage you to contribute to this community...

  3. MAPK Plasmids

    Type
    Collection
    ...extracellular signal-regulated kinases (ERKs), are serine/threonine-specific protein kinases. They regulate diverse...collection for community. If you have created plasmids containing MAPK and would like to add to this collection...

  4. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...directions from the target region, effectively turning Cas3 into a DNA “shredder” system that can cause... a boundary defined by anti-CRISPR proteins or running into other proteins bound to DNA, and the break...

  5. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...Cui, L., Bikard, D., & Van Teeffelen, S. (2018). Tuning dCas9’s ability to block transcription enables ...Dodd, I. B., & Shearwin, K. E. (2013). One-Step cloning and chromosomal integration of DNA. ACS Synthetic...

  6. CRISPR Plasmids and Resources

    Type
    Collection
    ...background information on CRISPR and the basics for planning your first CRISPR experiment. CRISPR 101 eBook...libraries are a powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used...

  7. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...window) External Collaboration Results in Student Learning Gains and Positive STEM Attitudes in CUREs. Callahan...undergraduate research experiences (CURE) impacts student learning and attitudinal outcomes: A study of the Malate...

  8. Retrovirus Plasmids

    Type
    Collection
    ...pMSCV PIG (Puro IRES GFP empty plasmid) MSCV For cloning and gene expression; select with puromycin or screen...pMSCV-IRES-mCherry FP MSCV A bicistronic IRES-mCherry containing retroviral plasmid Vignali 27995 TtRMPVIR CMV...

  9. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Technology SNAP-tag SNAP-tag is based on the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein... but reacts with benzylcytosine rather than benzylguanine derivatives to allow for dual labeling and visualization...

  10. Promega Plasmid Collection

    Type
    Collection
    ...sensitive gene expression analysis and fusion vectors containing tags such as NanoLuc, HaloTag, SmBiT, and LgBiT...editing outcomes, and conducting high-throughput screening in drug discovery. NanoLuc Fusions NanoLuc (Link...

  11. Biosensor AAV Preps

    Type
    Collection
    ...Sensors dLight RdLight GRAB_DA GRAB_rDA GRAB_gDA Serotonin Sensors psychLight iSeroSnFR GABA Sensors iGABASnFR...pAAV-hSyn-GRAB-gDA3m Syn GRAB_gDA3m none Constitutive 1 Li Serotonin Sensors 128485 pAAV-CAG-iSeroSnFR-Nlgn CAG iSeroSnFR...

  12. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...PRNP Dementia Susan Lindquist 22291 pcDNA3-FLAG-Synaptojanin 1-145 SYNJ1 Flag CMV Parkinsonism, early-onset...early-onset Pietro De Camilli 22292 pcDNA3-FLAG-Synaptojanin 1-170 SYNJ1 Flag CMV Parkinsonism, early-onset Pietro... Pietro De Camilli 22293 pEGFPC1-FLAG-Synaptojanin 1-145 SYNJ1 GFP, Flag CMV Parkinsonism, early-onset...early-onset Pietro De Camilli 22294 pEGFPC1-FLAG-Synaptojanin 1-170 SYNJ1 GFP, Flag CMV Parkinsonism, early-onset... U6 Parkinson's Timothy Ryan 223563 pmCherry-Synaptojanin-1-145 SYNJ1 His, mCherry CMV Parkinsonism, early-onset...

  13. Ligation Independent Cloning

    Type
    Protocol
    ... Protocols Ligation Independent Cloning Ligation Independent Cloning You may also ...Independent Cloning (LIC) obviates the need for the time-consuming ligation step of traditional cloning methods... Independent Cloning Protocols...also like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Overview Ligation Independent...methods. In traditional cloning, base-pairing in the short overlapping regions (usually 4 bp) does not provide... restriction sites from the final product (no "cloning scars"). A “stuffer” sequence allows for electrophoretic...design. Search Addgene's collection of empty LIC cloning vectors Protocol Step 1: Design Your Primers Primer...

  14. Synthetic Biology - Strains

    Type
    Collection
    ...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

  16. Synthetic Biology - Metabolism

    Type
    Collection
    ...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

  18. Zinc Finger Consortium Reagents

    Type
    Collection
    ... Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease...

  19. NETRF

    Type
    Collection
    ...cancers. The NETRF believes that creating and maintaining a highly collaborative NET research community...
Showing: 121 - 140 of 987 results