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We narrowed to 145 results for: transfection

Showing: 121 - 140 of 145 results

  2. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...genome. While this may effectively serve as a transfection or transduction control, a different gRNA would...

  6. Plan Your Experiment

    Type
    Guide
    ...For easy-to-transfect cell types (e.g. HEK293 cells), transfection with standard transfection reagents may...or gRNA in a mammalian cell line that can be transfected at high efficiency Lentiviral transduction Cas9... mammalian cell lines Useful for difficult to transfect cell types, can also be used in vivo A common ...

  7. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...generation R-mAbs plasmids, production of R-mAbs in transfected cells and their validation can be found below...inexpensively generate R-mAbs by transiently transfecting the plasmids into mammalian tissue culture cells... plasmids can be used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target Target...following plasmids can be used to produce scFvs from transfected mammalian cells. Addgene ID Plasmid Hybridoma...plasmids can be used to produce nanobodies from transfected bacterial cells. Addgene ID Plasmid Hybridoma... recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells....

  10. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...transfer, packaging, and helper plasmid) are transfected into cells which then act as in vitro factories...rare possibility that recombination between the transfected plasmids could lead to the production of replication-competent... AAV Vector topics Addgene Protocols General Transfection AAV Production in HEK293T Cells AAV Purification...and E1B19K genes provide a helper function for transfection-based adeno-associated virus vector production...DNA into eukaryotic cells using viral vectors. Transfection The process of artificially introducing foreign...

  12. Adenovirus Guide

    Type
    Guide
    ...rare possibility that recombination between the transfected adenoviral transfer plasmid and the E1 region...HEK293 or HER911 cell lines expressing E1 are transfected with the linearized construct, and rAdV vectors...packaging space. However, these constructs must be transfected into HER911 E4+ cells for rAdV vector production...DNA into eukaryotic cells using viral methods. Transfection The process of artificially introducing foreign...

  13. Lentiviral Vector Guide

    Type
    Guide
    ...three or four) are transfected into HEK293T cells using your preferred transfection method. The HEK293T... third-generation) can be used in transient transfections, but expression will be greatly limited due ...lentiviral plasmids cannot be used in direct transfections for transient expression of a transgene. For...

  14. Fluorescence Titering Assay

    Type
    Protocol
    ...brands and lots of FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to...

  15. Immunocytochemistry

    Type
    Protocol
    ...you know expresses the protein, such as cells transfected with a plasmid to express the protein of interest...

  16. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...above (envelope, packaging, and transfer) are co-transfected into the HEK293T packaging cell line. This system... number of plasmids that are required in the transfection step of the viral packaging cell line. For example...

  17. Colony Formation Titering Assay

    Type
    Protocol
    ...brands and lots of FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to...

  19. Western Blot

    Type
    Protocol
    ...you know expresses the protein, such as cells transfected with the protein of interest, and a negative ...

  20. Antibody Guide

    Type
    Collection
    ...hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express antibodies....
Showing: 121 - 140 of 145 results