We narrowed to 1,073 results for: TOR;

...GFP Aequorea victoria Mouse IgG2a 188167 Anti-IP3 receptor, type 1 [L24/21R] IP3 receptor, type 1 Rat ...in a new window) together with the laboratory of NeuroMab Director, Dr. James Trimmer, have produced numerous...Anti-GluA1/GluR1 glutamate receptor [N355/1R] GluA1/GluR1 glutamate receptor Rat Mouse IgG2a 114484 Anti-Stonin... IgG2a 114488 Anti-Dopamine D3 receptor [N331/19R] Dopamine D3 receptor Human Mouse IgG2a 114490 Anti-...Anti-GluN2B/NR2B glutamate receptor [N59/36.1R] GluN2B/NR2B glutamate receptor Rat Mouse IgG2a 114509 Anti-Pan-FHF-A...Anti-GluK5/Grik5/KA2 kainate receptor [N279B/27R] GluK5/Grik5/KA2 kainate receptor Rat Mouse IgG2a 149452 Anti-Kirrel3... IgG2a 177509 Anti-Dopamine D2 receptor [N186/29R] Dopamine D2 receptor Rat Mouse IgG2a 177510 Anti-SAP102...

...INSR Insulin receptor INSRR Insulin receptor-related receptor IRS IRS1 IRS2 Insulin receptor substrate JUN...transcription factor ECT2 Epithelial cell transforming 2 EGFR Epidermal growth factor receptor EIF4EBP EIF4EBP1...proto-oncogene, receptor tyrosine kinase MLST8 MTOR associated protein, LST8 homolog MTOR Mechanistic target...Ribosomal protein S6 kinase B1 RPTOR Regulatory associated protein of MTOR, complex 1 SAV1 Salvador family... Frederick National Laboratory for Cancer Research . Frederick National Laboratory for Cancer Research...Eukaryotic translation initiation factor 4E binding protein ERBB2 Erb-b2 receptor tyrosine kinase 2 ERK MAPK1...

...eYFP E2 regulatory element Activator C1V1 Dimidschstein 135634 pAAV-S5E2-ChR2-mCherry E2 regulatory element... Viral Vector Packaging Service AAV Caltech Systemic Capsids Viral Vector Packaging Service...CLOVER Center’s repository of published capsid use (Link opens in a new window) . Viral Vectors 101: Systemic...dTom-nlsdTom E2 regulatory element dTomato Control Dimidschstein 135631 pAAV-S5E2-GFP-fGFP E2 regulatory element...mCherry Syn Activator, Cre-dependent DREADD Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor, Cre-dependent...Roth 135635 pAAV-S5E2-Gq-P2A-dTomato E2 regulatory element Activator DREADD Dimidschstein Biosensors 104491...EF1a Activator, Cre-dependent ChR2 Deisseroth 127090 pAAV-CAG-DIO-ChR2(H134R)-eYFP CAG Activator, Cre-...

...generation vectors for transcription factor mediated differentiation of iPSCs into cortical and motor neurons...lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral vector for "hit...lentiviral vectors for human iPSC generation OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming...Fibroblasts Hematopoietic Progenitor Cells Lentiviral Human Cooperative Transcription Factor Induction Mediates...Fibroblasts Oligodendrocyte Progenitor Cells Lentiviral Mouse Transcription factor-mediated reprogramming ...Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells. Methods. 2015 Nov...iPSCs Cortical or Lower Motor Neurons CRISPR/TALEN Human Transcription Factor-Mediated Differentiation...

...Dopamine Optogenetics Activators Inhibitors Bidirectional DREADDs Activators Inhibitors Other Molecular Tools... Viral Vector Packaging Service AAV Retrograde AAV Viral Vector Packaging Service: Retrograde...the Alla Karpova and David Schaffer laboratories. These viral vector preparations were produced with the...EYFP Syn Activator Optogenetics Karl Deisseroth 28017 AAV-CAG-hChR2-H134R-tdTomato CAG Activator Optogenetics...CamKII Activator Optogenetics Karl Deisseroth 55639 pAAV-Ef1a-fDIO hChR2(H134R)-EYFP EF1a Activator Optogenetics...H134R)-GFP Syn Activator Optogenetics Edward Boyden 59170 pAAV-Syn-Chronos-GFP Syn Activator Optogenetics...pAAV-Syn-ChrimsonR-tdT Syn Activator Optogenetics Edward Boyden 83898 pAAV-mDlx-ChR2-mCherry-Fishell-3 mDIx Activator Optogenetics...

...contains modified pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based ...engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp...UTRs are included in the vector to enhance expression of the message. Both vectors utilize homodimeric FokI...the Golden Gate TALEN and TAL Effector Kit. These modified pFUS vectors can reduce the number of module...Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression/mRNA synthesis vectors containing... These new destination vectors can be used to create TALE-transcription factors and are used at the final... of polymorphic amino acid repeats in the TAL effectors. View Addgene's TALEN Guide. These collections...

...classes of base editors were cytosine base editors (purine-to-purine) and adenine base editors (pyrimidine-to-pyrimidine...base editors that can catalyze base transversions. These editors are based on cytosine base editors and...finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required scientists ...base editors, the evolved TadA domain is fused to Cas9n or dCas9 to create the adenine base editor. The...systems use a type V-K Cas effector, Cas12k. No matter the system, the Cas effectors lack nuclease activity...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...co-expression of epitope-tagged dCas9 and antibody-activator effector proteins; long-term imaging of proteins in...

...about viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral ...lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. Both lentiviral...Base editor (dCas9 or Cas9 nickase fusions with different base editing proteins) Many base editors fused...fusion with transcriptional activator (such as dCas9-VP64) Many different activator systems exist, including...base editors exert their functions within a limited window from the PAM sequence, and base editor variants...CRISPR protocols from Addgene depositors ). Browse empty gRNA expression vectors . Select Your Expression ... CRISPR plasmids . Delivery Using Viral Vectors Viral vectors are a great option, as they offer high expression...

...full sequencing of the final viral vector preparation. These viral vector preparations were produced with...Intravitreal delivery of a novel AAV vector targets ON bipolar cells and restores visual function in a mouse model...of Primary Human Hematopoietic Stem/Progenitor Cells by AAV6 Vectors: Strategies for Overcoming Donor-Variation...quality AAV preps from select plasmids in the repository. Browse the University of Florida serotype testing... Viral Vector Packaging Service AAV University of Florida Serotype...Serotype Testing Panel for the Eye and Brain Viral Vector Packaging Service: University of Florida Serotype ...

... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles... Viral Vector Packaging Service Viral Production Viral Production...vivo studies. Preparations are then aliquoted and stored at -80 °C. Titer Titering is either performed by...to determine which facility produced your viral vector lot. At Addgene, AAV particles are titered by droplet...

...cells expressing these engineered receptors. Chemogenetic receptors are popular in neuroscience, where...pathway the engineered receptor is coupled to and the specific cell type where the receptor is expressed, these...contains two main classes of chemogenetic receptors: Designer Receptors Exclusively Activated by Designer Drugs...Selective Actuator Modules (PSAMs). DREADDs are the most widely used chemogenetic receptors and are G-...Gi) and KORD are inhibitory. PSAMs are engineered α7 nicotinic acetylcholine receptor (nAChR) domains ...opto-chemogenetic actuator. For more detailed descriptions of chemogenetic receptors, ligands, and how...Chemogenetics plasmids encode genetically engineered receptors that are activated by specific small molecules...

...Adeno-Associated Viral (AAV) Vector Guide All Viral Plasmids Viral Vectors 101 eBook Viral Vectors 101 Youtube Videos... AAV vector production suitable for a variety of serotypes and applications. Read our AAV vector guide...production, and viral safety. AAV Vector Guide Want to save time? Our Viral Vector Packaging Service has hundreds...plasmids) used by Addgene's Viral Vector Packaging Service to produce AAV vectors for all available serotypes...contains helper plasmids in Addgene's repository for AAV vector production. ID Plasmid Description PI ..., packaging, and helper plasmids in Addgene's repository. Viral...AAV Helper Plasmids Adeno-associated viral (AAV) vectors offer precise delivery of genetic material across...

... in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9...Expresses dCAS9-VP64 activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator helper complex with...lentiviral vector encoding dCAS9-VP64 with 2A Blast resistance marker 61426 : lentiviral vector encoding...Nature 2015). The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9, ...61408 : Targeting vector for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling...SpCas9 or SpCas9n, and the single guide RNA. The vector can be digested using BbsI, and a pair of annealed...indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold. The oligos are designed...

...donor) mRuby2 (acceptor) FRET Michael Davidson 18856 pGWF1 ECFP (donor) Venus (acceptor) FRET Wolf Frommer...donor) Citrine (acceptor) FRET Wolf Frommer 65617 pFLIP42 mCerulean (donor) Citrine (acceptor) FRET Wolf Frommer...CRISPR Guide Bacteria are commonly used to create, store, and replicate plasmids of all types, but beyond...strains such as E. coli BL21(DE3) are used as biofactories to express plasmids and produce proteins in ... TrxA, Mocr, NusA Cleavage sites: TEV protease, factor Xa, enterokinase, thrombin Signal peptides for ...expression in E. coli , Bacillus subtilis , and Pichia pastoris . The EcoFlex MoClo Toolkit (Addgene #1000000080...express your protein of interest fused to FPs and monitor their localization in live cells. ID Plasmid Tags...

...quantum yield, λ Aem : Acceptor maximum emission wavelength (nm), ε A : Acceptor exctinction coefficient...transferred from an excited donor fluorophore to an acceptor. Because this process is extremely sensitive to...exciting the donor and detecting the emission of the acceptor. Alternatively, the fluorescence lifetime (the...each protein is separately fused to a donor or acceptor molecule (also referred to as intermolecular or...conformational changes within a protein where the donor and acceptor are both fused to the same protein (also referred... cloning backbones and control plasmids. Donor Acceptor λ Dex QY D λ Aem ε A QY A R 0 J(λ) Plasmids mTagBFP...3.1 pmTurquoise2-N1 , pCS2+mNeonGreen-C Cloning Vector , mNeonGreen-mTurquoise2 CyPet YPet 435 0.51 530...

...never happen. Yet with transcription activator-like (TAL) effectors, scientists in several biological disciplines... and help can be found at TALeffector Resources Center (www.taleffectors.com) . Whether you work in a ...between two distinct ZF target sites. (B) A TAL effector nuclease (TALEN). Similar in principle to the ...published the nucleotide recognition code of the TAL effectors, which were isolated from the plant bacterial ...that determines which single nucleotide the TAL effector will recognize: ( Figure 1B ) HD targets cytosine... in your average genome are every 500 bp. TAL effectors have some restrictions (for example, the target...determining the importance of context for each TAL effector within an array, but early studies suggest the...

...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...reporting on GPCR activity. AIMTOR : BRET biosensor for live imaging of mTOR signaling. Additional Resources...made luciferase a popular tool for scientists. Regulatory elements such as promoters, enhancers and untranslated...backbone plasmids into which you can clone your regulatory element or gene of interest into to create a ...Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest upstream...and renilla_polyA Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions upstream...212935 pGL4.84(hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating regions controlling transcription...

... Tumor necrosis factor receptor superfamily, member 10b E2F-1 E2F transcription factor 1 Fas Fas cell ... PAI Serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 PERP...p14ARF Cyclin-dependent kinase inhibitor 2A p21 Cyclin-dependent kinase inhibitor 1A p48 Damage-specific DNA...plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor activated by cellular stress...Pathway You May Also Like: Cancer Pathway ORF Kit mTOR pathway Ras pathway Background p53 Pathway Plasmids...List References Background p53 is a transcription factor and tumor suppressor activated by cellular stresses..., which disrupt its interaction with negative regulators, increase its stability and DNA binding activity...

...technologies. Penn Vector Core AAV Vectors Many AAV vectors from the Penn Vector Core have been transferred to Addgene...the viral vector prep you need to get your experiments started more quickly. Each viral vector is produced...when you receive your viral vector from Addgene, with details about storage. Biosafety Guide Documentation...biosafety of viral vectors, and general biosafety guidelines. Addgene Blog: Viral Vector Posts Content from...aspects of viral vectors, including troubleshooting advice and explanations of viral vector technologies....of particular Penn Vector Core catalog items. Virus-associated DNA Each viral vector service request will... Viral Vector Packaging Service Addgene offers flexible packaging...

...Resources Improved Gateway binary vectors — High-performance vectors for creation of fusion constructs...Genome Editing Gene Silencing Reporters Addgene’s repository contains many plasmid tools that were specifically...expression. Cloning and Synthetic Biology Expression Vectors The table below highlights plasmids that can be...collections are designed for creating plant expression vectors. ID Kit Description Cloning Type PI 1000000036 ...two-hybrid and bacterium-to-plant translocation vectors. Partially compatible with GoldenBraid. Golden ...functional parts for assembling plant transformation vectors, compatible with MoClo. Golden Gate Daniel Voytas...Level 0 parts for plant transformation, including regulatory elements, coding sequences, basic CRISPR parts...