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We narrowed to 878 results for: ids

Showing: 141 - 160 of 878 results

  1. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ... It’s possible to associate the depositor’s ORCID ID with the deposit, and a DOI is added to the deposit...protocols of molecular biology techniques Find plasmids at Addgene Make your work more accessible by depositing...

  2. Tips for arabidopsis transformation

    Type
    Blog Post
    ...https://books.google.at/books/about/Arabidopsis.html?id=IfZAMNPWVk4C&redir_esc=y (Accessed: 21 September ....org Find plant plasmids Visit our plant resource page Deposit arabidopsis plasmids with Addgene ...options, but here are a couple of my favorites. Plasmids from the Tsuyoshi Nakagawa lab are binary gateway...prefer Golden Gate assembly, there are a series of plasmids from the Dinneny lab that additionally allow for...

  3. The Challenges of Cell Culture

    Type
    Blog Post
    ...Short Tandem Repeat (STR) profiling can be used to ID the cell line. The same test is so good that it is...Protein Localization Resources on Addgene.org Find Plasmids for Mammalian Cells ...

  4. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...Storage of Plasmids Recovering Plasmid DNA from Bacterial Culture Background Information Plasmids can carry...presence of an antibiotic resistance gene on a plasmids allows researchers to easily isolate bacteria ...for 12-18 hr in a shaking incubator. Note: Some plasmids or strains require growth at 30°C. If so, you ... the difference between high copy and low copy plasmids? The copy number refers to the number of copies... plasmid within a single bacterial cell. Large plasmids usually have a low copy number (approximately ...approximately 18-30 hr). On the other hand, smaller plasmids can be present in large numbers, 50 or more per... and have a high copy number. High copy number plasmids should only need to be grown for 12-16 hr on average...

  5. CRISPR Library Amplification

    Type
    Protocol
    ...libraries contain tens to millions of different plasmids in a single sample. They are often used for screening...throughput multiplexed experiments. These pooled plasmids differ by only a short region of DNA: guide RNAs...can all impact the representation of individual plasmids in the pooled library during amplification. This...results for libraries up to 200,000 individual plasmids and can be scaled according to the actual number...restriction digest protocol here ). Lentiviral plasmids can recombine between their LTRs resulting in ...sufficient for libraries up to 200,000 individual plasmids. For larger libraries scale the number of cells...dramatically increases the chances of individual plasmids being lost from the pool and/or increasing the...

  6. Protocol - Bacterial Transformation

    Type
    Protocol
    ...both storing and replicating plasmids. Because of this, nearly all plasmids (even those designed for mammalian...efficient at taking up larger plasmids. If you need to transform large plasmids, it is a good idea to use ...introduced into a cell. Transformation of bacteria with plasmids is important not only for studies in bacteria ...very small amounts of DNA or if you're multiple plasmids at once. To save money, many labs also make their...few if any transformants when transforming large plasmids (>10 kb) or BACs, what can I do? Chemically competent...

  7. Centrifugation

    Type
    Protocol
    ...re-mix liquids. In cases where you are separating solids from a liquid, pellets represent the solids in your... speed. Being able to separate solids from a liquid or separate liquids of different densities is essential...

  8. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ... Storage of Plasmids Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids You may also... stocks are important for long-term storage of plasmids. Although you can store your plasmid DNA at -20...also create bacterial glycerol stocks of their plasmids. This way, when you want to make more plasmid ...

  9. Video Library

    Type
    Protocol
    ...Storing and Handling Addgene Plasmids What to do after you receive your plasmids from Addgene Instructions...Instructions for Storing and Handling Plasmids Genomic Deletions with CRISPR Generate genomic deletions in mammalian...Center How to Deposit Your guide to depositing plasmids with Addgene Depositing Page Career Videos (Link...

  10. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...correct pipettor and pipette tip, how to dispense liquids, and how to handle the pipette Watch the Video!...Bacterial Glycerol Stocks Store bacterial strains or plasmids for long term use Watch the Video! DNA Purification... Protocols for constructing and analyzing your plasmids. Name Description (Link opens in a new window)...window) Link to Video Restriction Cloning Assemble plasmids by restriction digest and ligation Cloning by PCR...

  11. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...desired insert (e.g., mScarlet).   Plasmid ID Plasmid Name Tags 69090 pMJ915 MBP (N...editing. How to use Addgene plasmids for Easi-CRISPR You can use Addgene plasmids to create a repair template...out our CRISPR Guide Find Plasmids for Cas9 Purification Find CRISPR Plasmids for Mammalian Expression ...described in Figure 2.  You can also use Addgene plasmids to purify Cas9 protein. The table below contains...design Easi-CRISPR repair templates using Addgene plasmids. Design PCR primers to add T7 promoter-left homology...

  12. Protocol - How to Streak a Plate

    Type
    Protocol
    ...will minimize the chance of having a mixture of plasmids in your purified DNA. This protocol explains how...overnight (12-18 hours) at 37 °C. Pro-Tip Some plasmids or bacteria need to be grown at 30 °C instead ... 37 °C. This is often true for large unstable plasmids, which sometimes recombine at 37 °C. Be sure to...

  13. Pipetting Protocol

    Type
    Protocol
    ...correct pipettor and pipette tip, how to dispense liquids, and how to handle the pipette....pipettes that have different mechanisms to disperse liquids, this protocol is focused on single channel pipettes...the liquid to stop flowing into the pipette tip. Liquids of various viscosity have different flow rates....

  14. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...suspension HEK293 cells with recombinant antibody plasmids using Polyethylenimine Max as a transfection reagent...General Considerations For antibody-expressing plasmids containing the SV40 origin of replication use ... SV40 large T antigen. For antibody-expressing plasmids containing the Epstein-Barr virus origin of replication...

  15. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids B. Designing shRNA Oligos for pLKO.1 B.1 Determine...response element. A.3 Related Products The following plasmids available from Addgene are recommended for use... the long terminal repeats found in lentiviral plasmids, Addgene currently recommends using a strain that...Top D. Screening for Inserts You may screen for plasmids that were successfully ligated by restriction ...

  16. Pouring LB Agar Plates

    Type
    Protocol
    ...also like... Plasmids 101 Blog Posts Mol Bio Protocols Cloning Protocols Introduction Plasmids can carry ...effective at selecting only bacteria containing the plasmids of interest (for example, fewer satellite colonies...

  18. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ... plate and select transformed cells containing plasmids differing in their resistance genes, as one does...effective at selecting only bacteria containing the plasmids of interest (for example, fewer satellite colonies...

  19. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...insert in a 5,000bp backbone, but there are many plasmids out there that, when digested with restriction...restriction enzyme based technique for verifying plasmids like this is "plasmid fingerprinting", where you...

  20. Gibson Assembly Protocol

    Type
    Protocol
    ...efficient and effective method for the assembly of plasmids, and molecular biologists now use this method ... background and enrich for correctly assembled plasmids by designing primers to split an antibiotic resistance...
Showing: 141 - 160 of 878 results