We narrowed to 437 results for: ERG
-
TypeBlog Post...delivered and expressed transiently using a standard detergent, Polyethylene Glycol (PEG), although the application...
-
10 Steps to a Perfect Science Talk
TypeBlog Post...about the problems you are addressing by using an energetic tone of voice. Your goal is to be conversational... -
Site Directed Mutagenesis by PCR
TypeBlog Post...site, digestion with the appropriate enzyme will merge two smaller bands visible in a digest of the unmodified... -
Evolution of Lab Techniques
TypeBlog Post...at Harvard University currently studying the serotonergic system and behavior. References 1. Southern... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post...before hand, you’re saving the institute time and energy that would typically be wasted going through some... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...-1.0 expresses RFP when the construct has not undergone recombination. This default state was disproportionately... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...from AAV2 and engineered to infect neurons and undergo retrograde transport, traveling up the axon to ... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...publication at Addgene. McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, Kramer A, Martens A, Edwards... -
A Control for All Seasons
TypeBlog Post...identical protocols that the experimental samples undergo. If the antibody is specific, then the target signal... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post...you can use Color>Split Channels and then Color>Merge Channels into whatever combination of colors you... -
Plasmids 101: Repressible Promoters
TypeBlog Post....89.12.5547. PMID: 1319065; PMCID: PMC49329. Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection... -
Antibodies 101: The Basics of Western Blotting
TypeBlog Post...wet transfer system the sandwich is completely submerged in a tank filled with a transfer buffer formulated... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...Goodwin LO, Morgan JL, Juan WC, Roca X, Ong ST, Bergstrom DE (2018) Viable Mice with Extensive Gene Humanization... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog Post...exonic regions. If the cell’s chromatin structure undergoes significant rearrangement (such as when a stem... -
CRISPR Guide
TypeGuide...cleavage. Generating a Knockout Using CRISPR Cas9 undergoes a second conformational change upon target binding...stability PEmax — optimized PE architecture; increased synergy between PE enzymes and epegRNAs for enhanced editing... ssisted T argeting), developed by Samuel H. Sternberg’s lab . Many of these systems are based on naturally...argeting E lements), developed by the Abudayyeh - Gootenberg lab , also builds off prime editing. PASTE uses...Welch, M. M., Sousa, A. A., Harrington, L. B., Sternberg, S. H., Joung, J. K., Yildiz, A., & Doudna, J.... S., Okazaki, S., Noda, T., Abudayyeh, O. O., Gootenberg, J. S., Mori, H., Oura, S., Holmes, B., Tanaka...Ran, F. A., Cong, L., Yan, W. X., Scott, D. A., Gootenberg, J. S., Kriz, A. J., Zetsche, B., Shalem, O.,... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...professor at the University of California, Santa Cruz. Emerging CRISPR/Cas9 editing technologies have transformed... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post...Biotechnology. References Degeling MH, Maguire CA, Bovenberg MSS, Tannous BA (2012) Sensitive Assay for Mycoplasma... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH (2018) An Alternate Route for Adeno-associated... -
Transferable Skills Guide: Teamwork
TypeBlog Post...experience on a successful scientific team came as an undergrad at Worcester Polytechnic Institute. Though some... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog Post...CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post... FP-protein fusions (FP-chimeras) is that they undergo normal protein turnover. FP-chimeras are continuously... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post...elimination by T cells. Immunotherapy strategies that synergize with these positive impacts on the immune system... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post...Modular Vector Assembly Enables Rapid Assessment of Emerging CRISPR Technologies. Cold Spring Harbor Laboratory... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post... your own ligands brings up bad memories of undergraduate organic chemistry lab, don’t worry – many SNAP-tag... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...notify you that we have received them and they will undergo our quality control process to verify your plasmids... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...liver and ameliorates streptozotocin-induced hyperglycemia. Nat Med, 2000. 6(5): p. 568-72. PubMed PMID... -
Deep Dive: qPCR
TypeBlog Post...and experimental parameters (neighbor stacking energies, loop entropy effects, cation concentrations and... -
CRISPR/Cas9 FAQs Answered!
TypeBlog Post...mcb.18.1.93 Ran, F. A., Hsu, P. D., Lin, C., Gootenberg, J. S., Konermann, S., Trevino, A. E., Scott,... -
Chemogenetics Guide
TypeGuide...intracellular region of a turkey erythrocyte β-adrenergic receptor with a rat M3 muscarinic receptor. This... al., 2007 rM3D Rat M3 muscarinic & turkey β1-adrenergic G αs CNO* Increase cAMP Neuronal burst firing...VChR1 CTZ Na + , K + influx Neuronal activation Berglund et al., 2016 LMO7 mNeonGreen-eKL9h VChR1 CFz Na...68. PMID: 17360345 (Link opens in a new window) Berglund K, Clissold K, Li HE, Wen L, Park SY, Gleixner...Liggett SB (2001). Modification of the beta 2-adrenergic receptor to engineer a receptor-effector complex... -
Technical Design of a Western Blot
TypeBlog Post... most Bradford assays are not compatible with detergents like SDS, while BCA assays are not with denaturing... -
Optogenetics Guide
TypeGuide...thus expressed in all GABAergic neurons. In this case, the subpopulation of GABAergic neurons being activated...Optogenetics Resource Center Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K. 2005. Millisecond-timescale... -
Gamma-Retroviral Vector Guide
TypeGuide...LaFave, M. C., Varshney, G. K., Gildea, D. E., Wolfsberg, T. G., Baxevanis, A. D., & Burgess, S. M. (2014...Schnierle, B. S., Stitz, J., Bosch, V., Nocken, F., Merget-Millitzer, H., Engelstädter, M., Kurth, R., Groner... -
Lentiviral Vector Guide
TypeGuide... Pelayo, A., Shah, N. N., Kochenderfer, J. N., Norberg, S. M., Hinrichs, C., Highfill, S. L., Somerville...D. M., Chen, I. S., Hahn, W. C., Sharp, P. A., Weinberg, R. A., & Novina, C. D. (2003). Lentivirus-delivered... -
Guide to Using Pooled Libraries
TypeGuide...CRISPR library. Apply pooled library to cells. DIVERGE: Positive and negative screens POSITIVE screen:... -
Modular Cloning Guide
TypeGuide... in filamentous fungi such as Penicillium or Aspergillus species. MoClo Yeast Secretion and Display (YSD... -
Molecular Biology Reference
TypeGuide...more manageable DNA fragments. In 1952, Joshua Lederberg coined the term plasmid, in reference to any extrachromosomal... -
Antibody Guide
TypeGuide...against parasites and is responsible for driving allergic reactions such as anaphylactic shock Monomer with...