...leaving behind any accessory DNA sequences [2], [3]. Simply put, the piggyBac™ transposon containing a selection...cytotoxic for proliferating cells and provides a simple tool for conditional cell ablation.,” Proc. Natl...

... and anterograde transport, but they also have similar transport characteristics (velocities of movement.... 3. Aschauer, Dominik F., Sebastian Kreuz, and Simon Rumpel. "Analysis of transduction efficiency, tropism...

... short science policy career and this seemingly simple question is a moving target. There are a multitude...pandemic has foisted upon us. Action Items:  A simple place to look for science policy folks is on Twitter...

... assists PI with admin duties Staff scientist: similar role to a postdoc but more permanent Visiting scholar...because they had close relatives suffering from similar diseases. Others were inspired by the imminent ...

...Using in vivo optogenetic stimulations, they simultaneously recorded the electrical impulses of discrete.... PubMed Central PMCID: PMC6010559. Wiegert, J. Simon, et al. "Silencing neurons: tools, applications,...

...have been widely adopted due to their relative simplicity and low cost. The problem with these assays is...Mismatch Cleavage Assay Not Provided 5% Inexpensive, simple Low-throughput, low sensitivity Sanger Sequencing...

...CD45 expression turns out to be weak. Table 2: A simple flow panel to detect live, transduced, and CD45...reduce it whenever possible. Finally, we designed a simple panel to analyze transduced HEK 293T cells. Here...

...works in both mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate system was...://doi.org/10.1371/journal.pone.0016765. Moore, Simon J., et al. “EcoFlex: A Multifunctional MoClo Kit...

...discussed below. The software could also be used as a simple tool to rapidly find one or a few gRNAs suggested...screen and the appropriate phenotypic readout, similar mapping could be rapidly performed by targeting...

...infections have resulted in the onset of cancer. Similar patterns of cancer development are observed in ...cell deaths that help activate tumor responses. Similarly, some radiotherapies have caused increased death...

...cells  Cy5NTAc 32 25-45 GFP (fluorescein is similar)   Lumio Red Tc: CCPGCC 48 38-70 Intact Cells ...80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby enabling...

...cells [41]. The in vivo and in vitro methods are similar except that the vectors carrying the transdifferentiation...transdifferentiation is suited for switching cell types between similar lineages, reprogramming is more versatile and universal...

...infectious transgenic lentivirus is outlined in the simple schematic to the right. 3-4 plasmids are transfected...recommended that you use lentiviral transfer plasmids for simple transfections. Is it feasible to express cDNA from...Often times it is less time consuming and easier to simply add restriction sites onto the insert of interest...

...muscarinic-receptor based DREADDs. Compound 21 has similar potency as CNO, while DCZ is more potent than either...Yadav PN, Daigle TL, Urban DJ, Lee H-M, Sciaky N, Simmons A, Nonneman RJ, Huang X-P, Hufeisen SJ, Guettier...opens in a new window) Slaviero AN, Gorantla N, Simkins J, Crespo EL, Ikefuama EC, Tree MO, Prakash M, ...

... extraordinary ability category The EB-1A - is similar to national interest waiver criteria however the...beneficial during a career search. Doing these simple things will make it easier for you to find and ...

...nickase (Nishida et al., 2016). Target-AID acts similarly but not identically to BE3, modifying a 3-5 base...Cas9 (Grünewald et al., 2020). Another lab took a similar approach. Dali Li’s lab’s A&C-BEmax consists of...

...for review see Dietzschold et al., 2008). Herpes Simplex Virus (HSV) also migrates through neurons where...Allen Mouse Brain Connectivity Atlas website. A similar approach was used by researchers from the Janelia...

...genes that contribute to a phenotype. At their simplest level, pooled libraries are single preparations...parallel sequencing of millions of DNA fragments simultaneously in a single reaction. As discussed above, access...

...Packaging using 293T cells This method is very similar to lentiviral packaging methods. The three components...constructs. Why do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone...

...for transcription initiation. The -35 position, simply titled the -35 element, typically consists of the...sequences. Operons Although bacterial transcription is simpler than eukaryotic transcription bacteria still have...

..., add the various reagents to a single tube and simply flick the tube several times. This will help ensure...

...Innovation. An Integrin Antibody Toolkit from IPI Simon Moe, from Promega, who wrote a guide to selecting...

...This calculator can do the math for you and it’s simple to make adjustments for the type of tissue culture...

... me best?     But between these options, we simply can't decide! Abi, Lin, or Blottie? Which name ...

...off their decorating skills. To join the contest, simply embellish and adorn your lab space or your work...

...a way to show different aspects of the data simultaneously. That’s why I turned to dashboards. Dashboards...

...snowflakes, since snowflakes and viruses both exhibit similar forms of symmetry. 🎶Walking in a Winter Wonderlab...

...Louis Bengyella and Kwabena Duedu who were awarded similar grants back in June, Gladys will be using plasmids...

...showing that Cpf1 has lower off-target editing and similar on-target editing when compared to the most commonly...

...multiplex editing of genomes. It will also allow simultaneous activation and/or repression of multiple genes...

... and The New England Journal of Medicine have similarly curated their Ebola-related content. -And the ...

...specific paper. If you’re ready to start a deposit, simply click on “Deposit Materials” and the search bar...

...Neisseria meningitidis, Nme2Cas9, that recognizes a simple dinucleotide PAM (N4CC), lending to high target...

... that 20% of tagged proteins did not localize similarly to their untagged counterparts. These findings...

...Plasmids" from these citing articles. What's this? Simply put, if the authors of a "Citing Article" have ...

...mck-PGC-1a1 mice found it wasn’t that they were simply immune to stress altogether. Their muscles showed...

...event planning and execution. I am seeing many similar examples of science trainees taking a proactive...

...study shows that the CRISPR/Cas9 system enables simultaneous disruption of two target genes in one step without...

...was... pSpCas9(BB)-2A-Puro (PX459) V2.0       Similar to last year, the top requested plasmid from 2016...

...microscopes work as conventional microscopes and give a similar optical quality (magnification of 140X and 2 micron...

...metagenomic datasets to try to determine whether simpler and maybe smaller Cas systems could exist in nature...

...researcher. The future of DayCon DayCon and other similar events show how meaningful science outreach can...

...Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!) and we’ll take...

...had been humming along at 99 percent efficiency, simply stopped working. They finally traced the problem...

...Request, a service that dramatically expands and simplifies your viral vector options. Almost any AAV transfer...

...the forefront, no longer allowing myself to just simply exist without creating a storm. Given all the options...

...more sustainable one. The math on this is pretty simple: wash your tips once and you cut your tips cost...

...P1 phage. 2) The post describes an ingenious but simple technique to screen for proteins that could be ...

... a Science Communication group started. It's as simple as getting people together at a bar. Chapter 10...

...the guesswork out of protein purification through simple and efficient multiplexing. pCri plasmids are divided...