... AAV8, as well as AAV5 and AAVrg. Find the Cre-expressing viral preps here AAV-CAG-dLight1.3b from ...in serotype AAV5! Get dLight1.3b here Looking for a knock-out in your experiments? AAV-flex-taCasp3...available as a ready to use viral prep in serotype AAV5. Find AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin...SaCas9 ABE variants. It's also one of the smallest AAV-deliverable ABE.  Find the plasmid here Jesse Zalatan...the Deisseroth viral preps here Coming soon! New AAV serotypes from the Sanford Boye lab with enhanced...include AAV1, and AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato is now available in AAV1 and AAV8...Loren Looger Lab now available in AAV1. Get GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature...

...elements are inserted into an AAV transfer vector and used to generate AAV particles. Transient or stable...for conducting genome-wide screens using CRISPR. AAV transduction Only compatible with SaCas9 (packaging...or gRNA. Infects dividing and non-dividing cells. AAV is least toxic method for in vivo viral delivery....hard Viral delivery   Lentivirus Retrovirus AAV Cells Dividing or nondividing Dividing Dividing...

...future experiments.  Use Addgene’s AAV Data Hub. You can search the AAV Data Hub for tools to use in future...experiments. If you’ve used Addgene’s AAV tools, you can contribute data to the AAV Data Hub to help the research...

...expression for AAV CREATE libraries Gradinaru 206504 AAV-KP1 AAV-KP1 AAV packaging vector containing AAV2 rep and...codon Mali 166921 AAV-F in pAR-9 AAV-F AAV rep/cap plasmid. Capsid is mouse brain tropic AAV-F Maguire 170716...McCown 183749 kiCAP-AAV-BI30 AAV-BI30 AAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with...-KanR AAV-BI28 RepCap for AAV production Deverman 203533 iCAP-BI-48-KanR AAV-BI48 RepCap for AAV production...-KanR AAV-BI49 RepCap for AAV production Deverman 203535 iCAP-BI-62-KanR AAV-BI62 RepCap for AAV production...and partial AAV2 p5 promoter Kay 206512 AAV-LK03 AAV-LK03 AAV packaging vector containing AAV2 rep and LK03...and partial AAV2 p5 promoter Kay 206513 AAV-AM AAV-AM AAV packaging vector containing AAV2 rep and AM ...

...Addgene.org AAV Vector for TVA Expression AAV for Rabies Glycoprotein (G) expression  Find Retrograde AAV ...viruses typically used in neuroscience research (e.g., AAV) are useful for labeling the individual neurons they...17179932. Additional Resources on the Addgene Blog AAVs CREATed for Gene Delivery to the CNS and PNS Advanced...

...currently uses ddPCR for AAV titrating. You can learn all about ddPCR for AAV titration and find helpful...tricks in our blog post “Droplet Digital PCR for AAV Quantification.”   Multiplex PCR Multiplex PCR, as... nucleotide concentration of starting material AAV Titrations Allelic Variations Quantifying low abundance...resources on the Addgene blog Droplet Digital PCR for AAV Quantification Plasmid cloning by PCR  Plasmids 101...

...distributing a small but growing catalog of ready-made AAV and Lentiviral preps. This new Viral Service represents... lab from the Allen Institute have supported the AAV initiative from the beginning and have worked closely...Research Campus have provided valuable insight into AAV production. Finally, we are blessed to have an incredibly...Blog Check Out the Top Requested Lentivirus and AAV of 2016 Learn How You Can Use the Viral Service in...

... Protocols AAV Production in HEK293T Cells AAV Production in HEK293T...adeno-associated virus (AAV) in HEK293 cells. Protocols...HEK293T Cells You may also like... AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular Biology...Introduction This protocol can be used to produce AAV from one Five Chambers Cell-Stack (CS5) (Link opens... Vortex Reagents Adherent HEK293T cells (ideally AAV-293T clones) *Pro-Tip* While adherent, these cells...not use filters made of materials other than PES. AAV particles stick to many other surfaces, but do not...health of the HEK293T cells is critical for optimal AAV yield. Do not overgrow your cells. Pass the cells...

...in the lab Over-Agar Antibiotic Plating Protocol AAV Purification Purify adeno-associated virus from a... a preparation produced in mammalian cells AAV Purification by Iodixanol Gradient Ultracentrifugation ...workspace, and maintaining sterility while working. AAV Titration by qPCR Use qPCR to measure the physical...titer of your adeno-associated virus preparation. AAV Titration by qPCR Using SYBR Green Technology Protocol...

... Viral Service AAV Retrograde AAV Viral Service: Retrograde AAV As part of our Viral Service...catalog items. Browse Available Retrograde AAV See our retrograde AAV based on the functional catagories listed...Activity Category PI Controls 37825 AAV-CAG-GFP CAG GFP Control Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP...sensor Kim 104489 pGP-AAV-syn-jGCaMP7b-WPRE Syn GCaMP7b Calcium sensor Kim 104491 pGP-AAV-syn-FLEX-jGCaMP7s-WPRE...104493 pGP-AAV-syn-FLEX-jGCaMP7b-WPRE Syn GCaMP7b, Cre-dependent Calcium sensor Kim 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE...104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG GCaMP7f, Cre-dependent Calcium sensor Kim 104488 pGP-AAV-syn-jGCaMP7f-WPRE...104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn GCaMP7f, Cre-dependent Calcium sensor Kim 162374 GP-AAV-syn-jGCaMP8s-WPRE...

...Broad tropism - AAV-PHP.eB, AAV-PHP.AX, AAV-PHP.eC Cell type-biased: Astrocytes - AAV-PHP.AX with GRE ...Neurons - AAV.CAP-B10, AAV-PHP.N Broad - AAV-PHP.AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP....and NHPs: Broad tropism - AAV-MaCPNS2, AAV-PHP.S Sensory nervous system - AAV-MaCPNS1 Return to top...pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-...GENIE 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn Calcium sensor GCaMP Kim , GENIE 104488 pGP-AAV-syn-jGCaMP7f-WPRE...scientific community. Text Description for the AAV Decision Tree The AAV decision tree graphic above helps you ...

... Viral Service AAV AAV Made on Request Viral...Viral Service: AAV Made on Request Due to high demand for viral items, Addgene's AAV Made on Request Service...Addgene is offering a Made on Request AAV packaging service for AAV plasmids not currently available as ...only offered for AAV plasmids deposited at Addgene. We are unable to perform custom AAV preparations for...available through the AAV Made on Request viral service? We only offer packaging for AAV plasmids deposited...service. AAV Service Details Volume 1-1.5 mL in 100 µL aliquots Minimum Titer 5E12 GC/mL Serotype AAV1, AAV2...Information on Addgene's AAV Made on Request viral packaging service Viral...

...with AAV-S5E2-GCaMP6f, now available as a ready-to-use viral prep in AAV1!  New ready-to-use AAV prep ...Plasmids for delivering CRISPRi components by a single AAV vector are now available from Tonia Rex's lab.  vfCRISPR...Gether lab, now available as ready-to-use AAV vectors.   ...Rylan Larsen lab, now available in a ready to use AAV5 viral prep.   Flp-dependent DREADDs from the Gether...

...cell lines from a polyclonal pool of stable cells AAV Production in HEK293 Cells Produce adeno-associated...deliver your gene of to the target of your choice AAV Purification by Iodixanol Gradient Ultracentrifugation...preparation produced in mammalian cells Watch the Video! AAV Titration by qPCR Using SYBR Green Technology Use...adeno-associated virus preparation Watch the Video! AAV Titration by ddPCR Use ddPCR to measure the physical...

...Addgene's AAV are made from a selected set of plasmids that are currently in our repository. AAV are distributed...coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA Ian Wickersham 104052-AAVPHP.V1 pAAV-CAG-DIO-EYFP An AAV genome...Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn driven...enhancer element Gordon Fishell 99120-AAV1 pAAV-ihSyn1-tTA An AAV genome that expresses tTA from the hSyn1...of the tTA Viviana Gradinaru 99121-AAV1 pAAV-ihSyn1-DIO-tTA An AAV genome with Cre-dependent expression...of the tTA Viviana Gradinaru 99130-AAV1 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized...6 enhancer Viviana Gradinaru 99130-AAV8 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized...

... Viral Service AAV Biosensors Viral Service: Biosensor AAV Browse our AAV that express fluorescent...162376 pGP-AAV-syn-jGCaMP8f-WPRE Syn jGCaMP8f none Constitutive 1, 9, rg* GENIE 162379 pGP-AAV-syn-FLEX-...162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent 1, 5, 9, rg* GENIE 169258 AAV-hSyn-Soma-jGCaMP8f...176750 AAV-CamKIIa-jGCaMP8f-WPRE CamKIIa jGCaMP8f none Constitutive 1, 5, rg* Looger 176753 AAV-mDlx-jGCaMP8f-WPRE...162374 pGP-AAV-syn-jGCaMP8s-WPRE Syn jGCaMP8s none Constitutive 1, 5, 9, rg* GENIE 162377 pGP-AAV-syn-FLEX-jGCaMP8s-WPRE...162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG jGCaMP8s none Cre dependent 1, 9, rg* GENIE 169256 AAV-hSyn-Soma-jGCaMP8s...176752 AAV-CamKIIa-jGCaMP8s-WPRE CamKIIa jGCaMP8s none Constitutive 1, 9, rg* Looger 176755 AAV-mDlx-jGCaMP8s-WPRE...

... Viral Service AAV Serotype Testing AAV Viral Service: ...: Serotype Testing AAV These AAV encode fluorescent reporters and can be used to compare the tropism of...experiments. For more information, see our full AAV inventory or our AAV production page . Note: For a list of ...Description : Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made from plasmid...have the suffix (for example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description... 140 Add to Cart AAV Retrograde 37825-AAVrg.T 20 µL $ 140 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) ...

...provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of site... Viral Service AAV Recombinases Viral Service: Recombinases These AAV encode site-specific recombinases... gene expression. Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA Syn...none 5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1(S)-FlpO-bGHpA hSyn...140137 pAAV-EF1a-iFlpV EF1a none 1, PHPeB Zeng *AAVrg = retrograde serotype, produced with the AAV retro...Deisseroth 87306 AAV pEF1a-DIO-FLPo-WPRE-hGHpA EF1a none 1, 2, 5, 8, 9, rg* Zhang 75469 pAAV-EF1a-Flp-DOG-...pAAV-EF1a-Flp-DOG-NW EF1a none 1 Cepko 183412 pAAV-CAG-FlpO CAG none 1, rg* Janelia VCre AAV ID Name Promoter Fluorophore...

... as AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb...mRNA display (FingRs), Xue Han’s lab has developed AAV vectors to selectively visualize excitatory and inhibitory...AAV-PHPeb. Find the AAV prep here ...

...action. AAV: A Versatile Viral Tool for Gene Expression in Mammals Adeno-associated viruses (AAVs) are commonly...Cepko’s Lab at Harvard Medical School describes how AAVs function and indicates important things to consider...