...long been used for labeling, but new methods are emerging to use these fluorescent proteins to modulate ...

... are expressed from plasmids. These antibodies undergo application-specific validation and consistent ...

...Michel Sadelain James Thomson Tim Townes Robert Weinberg Knut Woltjen Shinya Yamanaka Feng Zhang About ...

...Multiple sgRNAs targeted to the same promoter act synergistically to activate gene expression. The dCas9 protein...

... Allen WE, Singh A, Kuksenko O, Abudayyeh OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, ...

...recombinant antibodies. These monoclonal antibodies undergo application-specific validation and quality control...

...In vitro transcription none, need Cas9 plasmid Sternberg L4440_BioBrick-sgRNA T7 BbsI, BsaI Ingestion by...

...monoclonal antibodies are produced in-house and undergo application-specific validation and quality control...

... and ethical synthetic biology JBEI - Joint Bio-Energy Institute Synberc - Synthetic Biology Engineering...

...8896-7. PMID:15264811 Stachelhaus T, Mootz HD, Bergendahl V, Marahiel MA.1998. Peptide bond formation in...

...monoclonal antibodies are produced in-house and undergo application-specific validation and quality control...

...antibodies. Detergents  Another common family of permeabilization reagents are detergents, such as Triton...these detergents avoid some of the negative effects caused by organic solvents. However, detergents can ...for what reagents to use - organic solvents and detergents.  Organic Solvents  As mentioned above, organic...Triton X-100, Tween-20, or saponin. Each detergent has different chemical properties that will impact the... X-100 and Tween-20 are examples of non-ionic detergents that interact with lipids and proteins in membranes...protein. B) Permeabilizing with the selective detergent, saponin, keeps membrane-associated proteins in... epitopes. C) Permeabilizing with a non-ionic detergent, like Triton X-100, can lose membrane-associated...

...Cyanobacteria Cyanobacteria Plasmids for Fungi Species Aspergillus sp. Cryptococcus sp. Plasmids for Prions Prions...

...promoter. cGAL and Split cGAL plasmids - Paul Sternberg Lab. Plasmids for the temperature-robust GAL4-...

...Jens Boch at the Martin-Luther-University Halle-Wittenberg and Adam Bogdanove at Iowa State University published...

...guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia University Medical Center. DNA...with the discovery of CRISPR-transposons by the Sternberg and Zhang labs (Peters et al., 2017; Klompe et...Elements by Guide RNA-Assisted Targeting) by the Sternberg group, and CAST (CRISPR-associated Transposase...the CRISPR-transposon systems developed by the Sternberg lab derives from Vibrio cholerae (V. cholerae ...CRISPR-transposons for bacterial genomes engineering In the Sternberg group’s initial report of VchINT, three separate...Vo! Leo Vo is currently a PhD candidate in the Sternberg Lab at Columbia University Medical Center. He ....     References: Halpin-Healy TS, Klompe SE, Sternberg SH, Fernández IS (2020) Structural basis of DNA...

... and what's it good for? Fluorescence resonance energy transfer (FRET) is a biophysical interaction between...fluorophore (the donor) is excited, it can transfer energy directly via resonance to the second fluorophore...fluorophore labeled and that these fluorophores can undergo FRET when they are near each other. How do you ...still develop a successful FRET-based assay since energy transfer only occurs when donor and acceptor are...acceptors >100 Å away from the donor doesn’t result in energy transfer and thus, your specific FRET signal is...donors never “see” FRET acceptors, and thus never undergo FRET. If this nonspecific labeling far exceeds ...experiments will define the maximum and minimum energy transfer values you can expect, thereby establishing...

...What is this FRET you speak of? Förster Resonance Energy Transfer (FRET) was originally described by Theodor... the alternative acronym Fluorescence Resonance Energy Transfer. Unlike the typical excitation and emission...fluorophore, FRET involves a non-radiative transfer of energy (i.e. no emitted photons) from the excited donor...fluorophore excitation by absorption of a photon 2. Energy transfer from the excited donor directly to the...spectrum of the acceptor fluorophore. Otherwise the energy transferred from the donor will not be able to ...of each other and be oriented appropriately for energy transfer via dipole-dipole interaction. The efficiency...M., et al. "Quantification of Förster resonance energy transfer by monitoring sensitized emission in living...

...have acquired divergent functions due to mutations accumulated over time? Undergraduates can help to answer...providing authentic research experiences to undergraduate students (1, 2), but educators face significant...Time project provides a framework for these undergraduate research questions. The idea is straightforward...cerevisiae. The two yeasts are thought to have diverged from a common ancestor ~350 million years ago.... have been largely conserved since the species diverged. Tips on using the Pathways Over Time plasmids... in strategies to incorporate research into undergraduate courses and in communicating science to the ...Advancement of Science. (2011). Vision and Change in Undergraduate Biology Education: A Call to Action, Washington...

...ortholog in zebrafish, this species has more recently emerged as a model system for human disease and translational...