...Genome engineering using the CRISPR-Cas9 system. Ran FA, et al. Nature Protocols Nov;8(11):2281-308; (2013...

...that better human health.     References Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X,...Like everyone else in the world, I (Wenning) was fascinated by CRISPR and rushed to test it. I still remember...

...https://doi.org/10.1016/s0764-4469(00)01250-6 Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F ...

...MGC138897 FAS Fas (TNF receptor superfamily, member 6) ALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6 ...member D EF7, OIT1 FASLG Fas ligand (TNF superfamily, member 6) APT1LG1, CD178, CD95L, FASL, TNFSF6 FGF1 fibroblast...TNFRSF6 FASLG Fas ligand (TNF superfamily, member 6) APT1LG1, CD178, CD95L, FASL, TNFSF6 FCER1G Fc fragment ...

...), the REAL-Fast assembly method (Reyon et al., manuscript submitted), and the FLASH (Fast Ligation-based...REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL, REAL-Fast and FLASH...designed TALENs, and assembly methods for REAL, REAL-Fast and FLASH Genome...2013) More detailed information about REAL, REAL-Fast and FLASH, including detailed and up-to-date protocols...construction of TALEN expression plasmids using REAL, REAL-Fast or FLASH assembly. This new version of ZiFiT Targeter...target that guide the user through the REAL or REAL-Fast assembly process or lists of plasmid ID numbers ...

...jGCaMP8 Fast Genetically Encoded Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically...Yulong Li Dopamine Ultrafast neuronal imaging of dopamine dynamics (dLight1.1-1.5) Ultrafast neuronal imaging...Wolf Frommer Glutamate Ultrafast glutamate sensors (iGluSnFR variants) Ultrafast glutamate sensors resolve...sensors (fRCaMP1/2, GECO1/2) The kinetic mechanisms of fast-decay red-fluorescent genetically encoded calcium...Red Calcium sensor with brighter fluorescence and faster kinetics (CaMPARI2) Improved methods for marking...detection (iAchSnFR) (Constitutive or Cre-dependent) A fast genetically encoded fluorescent sensor for faithful... Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell...

...effector repeat arrays assembled using the REAL, REAL-Fast or FLASH methods... Vectors TALEN Expression Vectors for REAL, REAL-Fast and FLASH You may also like... Keith Joung Lab plasmids...effector repeat arrays (assembled using the REAL, REAL-Fast or FLASH methods) can be cloned for expression as... TALENs. Additional details about the REAL, REAL-Fast, and FLASH assembly methods can be found on the ...

...Monomer pFast-FT-N1 - Mammalian Expression pTRE-Fast-FT - Mammalian Expression pBAD/HisB-Fast-FT - Bacterial...nm) Brightness pKa Maturation Structure Plasmids Fast-FT 403(blue), 583(red) 466(blue), 606(red) 14.9(...Plasmids - Includes tagging with CFP, YFP, and mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your...

...BACs, what can I do? Chemically competent cells are fast and easy to use, but are less efficient at taking...

... and FAQ Plasmid purification kits provide the fastest way to obtain a high concentration of clean plasmid...

...of PEG. Stirring under medium heat will promote faster dissolution. Adjust the pH to ~7.4. Autoclave or...

...Mouse IgG2a 177438 Anti-Pan-Neurofascin (extracellular) [A12/18R] Pan-Neurofascin (extracellular) Rat Mouse...Mouse 190485 Pan-Neurofascin (extracellular) scFv [A12/18] A12/18 scFv 2t Pan-Neurofascin (extracellular...channel Rat Mouse 206722 Pan-Neurofascin scFv [L11A/41] L11A/41 scFv Pan-Neurofascin Rat Mouse 206723 PARIS/...

... that contain a recombinant plasmid likely grow faster in the closed system of a crowded plate or culture...

...foundational optogenetics tool - they typically allow the fast depolarization of neurons upon exposure to light...compared to CrChR2 450 ChETA E123T mutation; creates faster kinetics but reduces photocurrent amplitude 490.... Red-shifted optogenetic excitation: a tool for fast neural control derived from Volvox carteri. Nat ...

...transgenesis Insect / Baculovirus Polyhedrin pFastBac LIC and pFastBac Dual LIC - Vectors from...

...files) or single-end reads (single file) in FASTQ format (fastq.gz files are also accepted) from a deep sequencing...

...TTGCCTTATTATCTGCGAaactttcggactgtccTTGAGGCTGTGTTGGAGA fascin-2b TAL3074 & TAL3075 TGCCCTCCAATGGCAGCAaagcccttaagcttcaATTCGGGCTTATTAATCA fastkd3 TAL3438...

... 65006 Bacteria BsaI yes, interfere S. pyogenes Koffas BPK764 65767 Bacteria BsaI yes, cut S. pyogenes...repressing multiple target genes in Escherichia coli. Koffas Do you have suggestions for other plasmids that...

... FUS_LC_withR FUS T7 ALS Nicolas Fawzi 139211 pFastBac1-LRRK2 WD40 LRRK2 His, MBP polH Parkinson's Hao...S473D OPTN GST-TEV ALS Sascha Martens 187830 pFastBac_Dual_GST-TEV-EGFP-TBK1 TBK1 GST, TEV-mEGFP-GG Linker...6His HTRA2 His T7 Parkinson's Eric Fischer 198033 pFastBac-GST-TEV-mCherry-TBK1 TBK1 mCherry, GST, Tev P10...P10 ALS Sascha Martens 198037 pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K TBK1 GFP, GST, Tev P10 ALS Sascha ...Sascha Martens 198072 pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666) TBK1 GFP, GST, Tev P10 ALS Sascha Martens...

...please contact Dr. John Calarco: jcalarco@fas.harvard.edu These plasmids are descirbed in: Heritable ...